Transcriptomics reveals amygdala neuron regulation by fasting and ghrelin thereby promoting feeding

Peters, Christian; He, Songbai; Fermani, Federica; Lim, Hansol; Mayer, Christian; Klein, Rüdiger

doi:10.1101/2022.10.21.513224

Cited by 8 publications

(23 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to these major cell types, our study revealed additional cell-type diversity in the mouse CEA, especially in the CeM, which had been largely underdefined. In addition, our molecularly defined mouse CEA cluster also matched well with two recent studies in mouse CEA ( O’Leary et al, 2022 ; Peters et al, 2022 ; Figure 1—figure supplement 3 ).…”

Section: Discussionsupporting

confidence: 87%

“…In this study, we only analyzed samples from male animals, and additional experiments will be needed in female animals to evaluate whether there are differences in molecularly defined cell types or their projections. However, scRNA-seq data from Peters et al, 2022 using CEA neurons of both male and female animals did not report sex-specific differences. Ultimately, combining EASI-FISH with fluorescent retrograde tracers enables the profiling of tens of thousands of cells for gene expression and projections.…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Multimodal mapping of cell types and projections in the central nucleus of the amygdala

Wang

Krabbe

Eddison

et al. 2023

eLife

View full text Add to dashboard Cite

The central nucleus of the amygdala (CEA) is a brain region that integrates external and internal sensory information and executes innate and adaptive behaviors through distinct output pathways. Despite its complex functions, the diversity of molecularly defined neuronal types in the CEA and their contributions to major axonal projection targets have not been examined systematically. Here, we performed single-cell RNA-sequencing (scRNA-Seq) to classify molecularly defined cell types in the CEA and identified marker genes to map the location of these neuronal types using expansion asisted iterative fluorescence in situ hybridization (EASI-FISH). We developed new methods to integrate EASI-FISH with 5-plex retrograde axonal labeling to determine the spatial, morphological, and connectivity properties of ~30,000 molecularly defined CEA neurons. Our study revealed spatio-molecular organization of the CEA, with medial and lateral CEA associated with distinct molecularly defined cell families. We also found a long-range axon projection network from the CEA, where target regions receive inputs from multiple molecularly defined cell types. Axon collateralization was found primarily among projections to hindbrain targets, which are distinct from forebrain projections. This resource reports marker gene combinations for molecularly defined cell types and axon-projection types, which will be useful for selective interrogation of these neuronal populations to study their contributions to the diverse functions of the CEA.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 91%

Multimodal mapping of cell types and projections in the central nucleus of the amygdala

Wang

Krabbe

Eddison

et al. 2023

eLife

View full text Add to dashboard Cite

show abstract

“…BLA Lypd1 neurons are found in three cell clusters in LA and acBA, complementary to BLA Etv1 neurons which are enriched in amBA and alBA. In slice electrophysiological recordings, BLA Lypd1 neurons showed increased firing rates in fasted animals, similar to our previous recordings of appetitive CeA neurons 57 . In vivo calcium recordings revealed that a large fraction of BLA Lypd1 neurons are active during food approach behavior, whereas smaller fractions of BLA Lypd1 neurons respond to fear and social stimuli.…”

Section: Discussionsupporting

confidence: 89%

“…The mechanism by which BLA Lypd1 neurons promote food intake remains to be explored. It is possible that BLA Lypd1 neurons directly synapse onto orexigenic CeA neurons including the recently described Ghrelin-responsive CeA Htr2a neurons 57 . It also remains to be tested whether the activity of BLA Lypd1 neurons is modulated by other internal states besides hunger and by environmental factors such a risk stimulus 35 .…”

Section: Discussionmentioning

confidence: 99%

Genetically- and spatially-defined basolateral amygdala neurons control food consumption and social interaction

Lim,

Zhang,

Peters

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The basolateral amygdala (BLA) contains discrete neuronal circuits that integrate positive or negative emotional information and drive the appropriate innate and learned behaviors. Whether these circuits consist of genetically-identifiable and anatomically segregated neuron types, is poorly understood. Also, our understanding of the response patterns and behavioral spectra of genetically-identifiable BLA neurons is limited. Here, we classified 11 glutamatergic BLA cell clusters and found that several of them were anatomically segregated in lateral versus basal amygdala, and anterior versus posterior regions of the BLA. Two of these BLA subpopulations innately responded to valence-specific, whereas one responded to mixed - aversive and social - cues. Positive-valence BLA neurons promoted normal feeding, while mixed selectivity neurons promoted fear learning and social interactions. These findings enhance our understanding of cell type diversity and spatial organization of the BLA and the role of distinct BLA populations in representing valence-specific and mixed stimuli.

show abstract

“…In rodents, 5HT2aR-expressing neurons in the central amygdala are responsive to fasting and modulate food consumption 47,48 . That HPCv 5HT2aR influence food intake through memory function is in line with reports in humans with Htr2a allele variants influencing hippocampal function [49][50][51] and work in rodents illustrating a role for 5HT2aR signaling in learning in memory processes [52][53][54][55][56][57] , and specifically in the HPCv 58 .…”

Section: Discussionmentioning

confidence: 99%

Ventral hippocampus neurons encode meal-related memory

Décarie-Spain,

Gu,

Lauer

et al. 2023

Preprint

View full text Add to dashboard Cite

The ability to encode and retrieve meal-related information is critical to efficiently guide energy acquisition and consumption, yet the underlying neural processes remain elusive. Here we reveal that ventral hippocampus (HPCv) neuronal activity dynamically elevates during meal consumption and this response is highly predictive of subsequent performance in a foraging-related spatial memory task. Targeted recombination-mediated ablation of HPCv meal-responsive neurons impairs foraging-related spatial memory without influencing food motivation, anxiety-like behavior, or escape-mediated spatial memory. These HPCv meal-responsive neurons project to the lateral hypothalamic area (LHA) and single-nucleus RNA sequencing and in situ hybridization analyses indicate they are enriched in serotonin 2a receptors (5HT2aR). Either chemogenetic silencing of HPCv-to-LHA projections or intra-HPCv 5HT2aR antagonist yielded foraging-related spatial memory deficits, as well as alterations in caloric intake and the temporal sequence of spontaneous meal consumption. Collective results identify a population of HPCv neurons that dynamically respond to eating to encode meal-related memories.

show abstract

Transcriptomics reveals amygdala neuron regulation by fasting and ghrelin thereby promoting feeding

Cited by 8 publications

References 84 publications

Multimodal mapping of cell types and projections in the central nucleus of the amygdala

Multimodal mapping of cell types and projections in the central nucleus of the amygdala

Genetically- and spatially-defined basolateral amygdala neurons control food consumption and social interaction

Ventral hippocampus neurons encode meal-related memory

Contact Info

Product

Resources

About