Transcripts of the nebulin gene from Ciona heart and their implications for the evolution of nebulin family genes

Nishikawa, Akira; Hanashima, Akira; Nakayama, Satoshi; Ogasawara, Michio; Kimura, Sumiko

doi:10.1016/j.gene.2019.144036

Cited by 1 publication

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“…Thus, lancelets are thought to express both lasp and nebulin from a single LASP/NEB that is similar to vertebrate LASP2/NEBL (see Figure 1b). Recently, Nishikawa et al, (2019) reported that the Ci NEB , coding Ascidiacea nebulin, is located on the same chromosome 1 as Ci LASP , albeit distantly (14.3 Mb). The LIM domain was not found in Ci NEB and transcripts encoding nebulin repeats and the SH3 domain are expressed in various muscle tissues.…”

Section: Introductionmentioning

confidence: 99%

The emergence of nebulin repeats and evolution of lasp family proteins

et al. 2021

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The LIM and SH3 domain protein (lasp) family, the smallest proteins in the nebulin superfamily, consists of vertebrate lasp‐1 expressed in various non‐muscle tissues, vertebrate lasp‐2 expressed in the brain and cardiac muscle, and invertebrate lasp whose functions have been analyzed in Ascidiacea and Insecta. Gene evolution of the lasp family proteins was investigated by multiple alignments, comparison of gene structure, and synteny analyses in eukaryotes in which mRNA expression was confirmed. All invertebrates analyzed in this study belonging to the clade Filasterea, with the exception of Placozoa, have at least one lasp gene. The minimal actin‐binding region (LIM domain and first nebulin repeat) and SH3 domain detected in vertebrate lasp‐2 were found to be conserved among the lasp family proteins, and we showed that nematode lasp has actin‐binding activity. The linker sequences vary among invertebrate lasp proteins, implying that the lasp family proteins have universal and diverse functions. Gene structures and syntenic analyses suggest that a gene fragment encoding two nebulin repeats and a linker emerged in Filasterea or Holozoa, and the first lasp gene was generated following combination of three gene fragments encoding the LIM domain, two nebulin repeats with a linker, and the SH3 domain.

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Section: Introductionmentioning

confidence: 99%