Transcutaneous Imaging of Rabbit Kidney Using 3-D Acoustic Wave Sparsely Activated Localization Microscopy With a Row-Column-Addressed Array

Tan, Qingyuan; Riemer, Kai; Hansen-Shearer, Joseph; Yan, Jipeng; Toulemonde, Matthieu; Taylor, Laura; Yan, Su; Dunsby, Christopher; Weinberg, Peter D.; Tang, Meng-Xing

doi:10.1109/tbme.2024.3426487

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2024

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Achieving Single Cell Acoustic Localisation with Deactivation Super Resolution

Smith,

Duan,

Yan

et al. 2024

Preprint

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Super-resolution optical microscopy enables optical imaging of cells, molecules and other biological structures beyond the diffraction limit. However, no similar method exists to super-resolve specific cells with ultrasound. Here we introduce Deactivation Super Resolution (DSR), an ultrasound imaging method that uses the acoustic deactivation of genetically encodable contrast agents to super-resolve individual cells with ultrasound as they navigate through structures that cannot be resolved by conventional imaging methods. DSR takes advantage of gas vesicles, which are air-filled sub-micron protein particles that can be expressed in genetically engineered cells to produce ultrasound contrast. Our experimental results show that DSR can distinguish sub-wavelength microstructures that standard B-mode ultrasound images fail to resolve by super-localizing individual mammalian cells. This study provides a proof of concept for the potential of DSR to serve as a super-resolution ultrasound technique for individual cell localization, opening new horizons in the field.

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