Transcutaneous Ultrasound Guided Intraovarian Injection in Rats (<i>Rattus norvegicus)</i>

Gomes, Etiele Maldonado; Santos, Endreo Alan Pail dos; Neto, Leonel Felix Leão; Padilha, Danielle R; Campos, Gabrielle C. S.; Thiesen, Roberto; Machado, Ingrid Rios Lima; Mestieri, Maria Ligia A

doi:10.30802/aalas-jaalas-20-000136

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…The length, width, and area of the ovaries were measured, and the presence and quantity of ascites in the abdomen and around the ovary were noted. The quantity of ascites in the peritoneum was graded as mild (I), moderate (II), and high (III) [ 17 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

Ultrasonographic and histopathological investigation of the effect of N-acetylcysteine on doxorubicin-induced ovarian and uterine toxicity in rats

Üstüner,

Yıldırım,

Macun

et al. 2024

J Ovarian Res

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Background This study aimed to investigate the mitigating effect of N-acetylcysteine (NAC) on doxorubicin (DOX)-induced ovarian and uterine toxicity in rats using laboratory tests, ultrasonographic (US) imaging, and histopathology analysis. Methods Forty-eight rats were divided into six groups (n = 8) as follows: Group A (control) (0.5 mL saline administered intraperitoneally [IP]), Group B (a single 10 mg/kg dose of DOX administered IP on day 1), Group C (a single 10 mg/kg dose of DOX administered IP 24 h before sacrifice), Group D (100 mg/kg of NAC administered IP for 21 days), Group E ( a single 10 mg/kg dose of DOX administered IP on day 1 and 100 mg/kg of NAC administered IP for 21 days), and Group F (100 mg/kg of NAC administered IP for 21 days and a single 10 mg/kg dose of DOX administered IP 24 h before sacrifice). The ovaries were examined using B-mode US on days 1, 14, and 21, and the histopathological examinations of the ovaries and the uterus were undertaken after sacrifice on day 22. Results Histomorphological analyses showed that ovarian weight decreased after DOX administration in Group B but not in Group E. US revealed a transient increase in ovarian size in Group B and E, reverting to baseline levels over time, as well as a progressive increase in peritoneal fluid in Groups B and E. Group B exhibited a significant decrease in the thickness of the endometrium and myometrium and uterine cornual length, which was not observed in Group E. Histopathological examination showed that DOX caused a decline in follicular count, especially in primordial, secondary, and Graafian follicles, and resulted in follicular atresia, predominantly in Group B. Destructive degeneration/necrosis and vascular changes were most prominently seen in the corpus luteum of Groups C and B. In NAC-treated rats (Groups E and F), although germ cell damage was present, atretic follicles and vascular changes, such as hyperemia and congestion, were reduced. The anti-müllerian hormone (AMH) level was the highest in Group F. Conclusions NAC, an antioxidant, attenuated DOX-induced gonadotoxicity in rats.

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Section: Methodsmentioning

confidence: 99%

Ultrasonographic and histopathological investigation of the effect of N-acetylcysteine on doxorubicin-induced ovarian and uterine toxicity in rats

Üstüner,

Yıldırım,

Macun

et al. 2024

J Ovarian Res

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“…Intraovarian percutaneous injection of 150 μl of solution was performed, guided per ultrasonography as previous described [ 27 ], in both ovaries, as follows: group GC (control) - saline solution; group G10 - calcium chloride 10% diluted in ethanol 95%; group G20 - calcium chloride 20% diluted in ethanol 95%. Previous practice on rats’ reproductive tracts showed that the volume of 150 μl of saline solution would be sufficient to fulfil the in rats of same age (90 days-old).…”

Section: Methodsmentioning

confidence: 99%

Calcium chloride diluted in ethanol 95% as female sterilizing agent: effect of transcutaneous delivery in rats

et al. 2022

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Background Different fertility control methods are investigated as a tool for population control of free-roaming animals. Chemical castration using calcium chloride has been widely studied over the years in males, but there are few studies related to its use in females. Therefore, we aimed to evaluate the local effects, as a potential chemosterilant, of two concentrations of calcium chloride diluted in 95% ethanol when administered by transcutaneous ultrasound-guided intraovarian injection in rats. In this study, 30 female Wistar rats were randomly divided into three treatment groups, which consisted of transcutaneous ultrasound-guided intraovarian injection of: 0.9% sodium chloride solution (GC); 10% calcium chloride diluted in 95% ethanol (G10); 20% calcium chloride diluted in 95% ethanol (G20). The animals were subdivided into two evaluation times, 15 days (n = 5 of each group) and 30 days (n = 5 of each group) after the intraovarian injection. The ovarian diameter was measured using ultrasound image prior and immediately after the injection and after the treatment period. Furthermore, animals’ clinical evaluation, estrous cycles assessment, macroscopic examination of the abdominal cavity and histological evaluation of the ovaries were performed. Results Ovarian ultrasound measurement revealed changes (p < 0.05) between ovarian diameters before and immediately after the injection in all treatments. Three animals in G20 had a small focal skin lesion at the injection site that evolved to total healing. Extended and abnormal estrous cycles were observed in G10 and G20. At gross examination, adhesions and ovarian cysts were noticed in both groups, G10 and G20. Also, the histopathology analysis revealed changes in ovarian architecture and vessel congestion in G10 and G20, but ovarian tissue damage was greater in the ovaries treated with the highest concentration (G20). Conclusions The results indicate that 20% calcium chloride diluted in 95% ethanol may be a potential agent for inducing sterilization in females and was possible to be minimally invasively delivered.

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Transcutaneous Ultrasound Guided Intraovarian Injection in Rats (Rattus norvegicus)

Cited by 2 publications

References 13 publications

Ultrasonographic and histopathological investigation of the effect of N-acetylcysteine on doxorubicin-induced ovarian and uterine toxicity in rats

Ultrasonographic and histopathological investigation of the effect of N-acetylcysteine on doxorubicin-induced ovarian and uterine toxicity in rats

Calcium chloride diluted in ethanol 95% as female sterilizing agent: effect of transcutaneous delivery in rats

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