Transdifferentiation in Animal Cells: Fact or Artifact?

Okada, Tamotsu

doi:10.1111/j.1440-169x.1986.00213.x

Cited by 45 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[7][8][9] Dedifferentiation is defined here as the loss of mature and functional characteristics from a partially or terminally differentiated cell type, and which, in some cases, may occur prior to trans-differentiation, or the change from one differentiated phenotype to another. 10,11 A minority compartment of b-cells within islets which demonstrated the capacity for dedifferentiation in vitro has been reported previously, with 1.4% 12 and 0.5% 13 of b-cells dedifferentiating in vitro from mouse, and <5% of human b-cells dedifferentiating in vitro using similar culture conditions, 7 although none of these studies characterized the rare plastic cells. These low rates of dedifferentiation might indicate that only rare b-cells are capable of survival and phenotypic transition, and potentially suggesting b-cell heterogeneity.…”

Section: Introductionmentioning

confidence: 84%

Insulin-positive, Glut2-low cells present within mouse pancreas exhibit lineage plasticity and are enriched within extra-islet endocrine cell clusters

Beamish

Strutt

Arany

et al. 2016

Islets

View full text Add to dashboard Cite

Regeneration of insulin-producing β-cells from resident pancreas progenitors requires an understanding of both progenitor identity and lineage plasticity. One model suggested that a rare β-cell sub-population within islets demonstrated multi-lineage plasticity. We hypothesized that β-cells from young mice (postnatal day 7, P7) exhibit such plasticity and used a model of islet dedifferentiation toward a ductal epithelial-cell phenotype to test this theory. RIPCre;Z/AP(+/+) mice were used to lineage trace the fate of β-cells during dedifferentiation culture by a human placental alkaline phosphatase (HPAP) reporter. There was a significant loss of HPAP-expressing β-cells in culture, but remaining HPAP(+) cells lost insulin expression while gaining expression of the epithelial duct cell marker cytokeratin-19 (Ck19). Flow cytometry and recovery of β-cell subpopulations from whole pancreas vs. islets suggest that the HPAP(+)Ck19(+) cells had derived from insulin-positive, glucose-transporter-2-low (Ins(+)Glut2(LO)) cells, representing 3.5% of all insulin-expressing cells. The majority of these cells were found outside of islets within clusters of <5 β-cells. These insulin(+)Glut2(LO) cells demonstrated a greater proliferation rate in vivo and in vitro as compared to insulin(+)Glut2(+) cells at P7, were retained into adulthood, and a subset differentiated into endocrine, ductal, and neural lineages, illustrating substantial plasticity. Results were confirmed using RIPCre;ROSA- eYFP mice. Quantitative PCR data indicated these cells possess an immature β-cell phenotype. These Ins(+)Glut2(LO) cells may represent a resident population of cells capable of forming new, functional β-cells, and which may be potentially exploited for regenerative therapies in the future.

show abstract

Section: Introductionmentioning

confidence: 84%

Insulin-positive, Glut2-low cells present within mouse pancreas exhibit lineage plasticity and are enriched within extra-islet endocrine cell clusters

Beamish

Strutt

Arany

et al. 2016

Islets

View full text Add to dashboard Cite

show abstract

“…Cell transformation or transdifferentiation has been defined as the direct conversion of one differentiated cell type into another differentiated cell type [78]. A sterling example of transdifferentiation in adult mice is the recent discovery by Zhou et al [79], who induced transdifferentiation of exocrine cells into β-islet cells of the pancreas using four main transcription factors needed for dedifferentiation.…”

Section: Transdifferentiationmentioning

confidence: 99%

The Urodele Limb Regeneration Blastema: The Cell Potential

Tweedell

2010

The Scientific World JOURNAL

View full text Add to dashboard Cite

The developmental potential of the limb regeneration blastema, a mass of mesenchymal cells of mixed origins, was once considered as being pluripotent, capable of forming all cell types. Now evidence asserts that the blastema is a heterogeneous mixture of progenitor cells derived from tissues of the amputation site, with limited developmental potential, plus various stem cells with multipotent abilities. Many specialized cells, bone, cartilage, muscle, and Schwann cells, at the injury site undergo dedifferentiation to a progenitor state and maintain their cell lineage as they redifferentiate in the regenerate. Muscle satellite reserve stem cells that are active in repair of injured muscle may also dedifferentiate and contribute new muscle cells to the limb blastema. Other cells from the dermis act as multipotent stem cells that replenish dermal fibroblasts and differentiate into cartilage. The blastema primordium is a self-organized, equipotential system, but at the cellular level can compensate for specific cell loss. It is able to induce dedifferentiation of introduced exogenous cells and such cells may be transformed into new cell types. Indigenous cells of the blastema associated with amputated tissues may also transform or possibly transdifferentiate into new cell types. The blastema is a microenvironment that enables dedifferentiation, redifferentiation, transdifferentiation, and stem cell activation, leading to progenitor cells of the limb regenerate.

show abstract