Transdifferentiation of Pigmented Epithelium Induced by the Influence of Lens Epithelium in Frogs

Lopashov, G. V.

doi:10.1111/j.1432-0436.1983.tb01298.x

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…In order to interpret our finding with these demonstrated results, we consider that the newly formed chick lens is a source of FGFs, specifically FGF1, FGF2, FGF 19 [called FGF15 in mouse] (Lovicu and McAvoy, 2005). In fact, lens tissue has been used in classical experiments as a source of FGF (Lopashov, 1983). FGF is a potent inducer of neural retinal cell fate in the optic tissue, and almost any member of the family can produce this result: FGF1 and 2 (Hyer et al, 1998;Park and Hollenberg, 1989;Pittack et al, 1991;Shimogori et al, 2004), FGF8 (Vogel-Hopker et al, 2000, and FGF9 (Zhao et al, 2001).…”

Section: Ciliary Body Tissue Is Identified At a Distance From An Fgf Sourcementioning

confidence: 71%

FGF-mediated induction of ciliary body tissue in the chick eye

Silva

Tiffin

Mima

et al. 2007

Developmental Biology

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Upon morphogenesis, the simple neuroepithelium of the optic vesicle gives rise to four basic tissues in the vertebrate optic cup: pigmented epithelium, sensory neural retina, secretory ciliary body and muscular iris. Pigmented epithelium and neural retina are established through interactions with specific environments and signals: periocular mesenchyme/BMP specifies pigmented epithelium and surface ectoderm/FGF specifies neural retina. The anterior portions (iris and ciliary body) are specified through interactions with lens although the molecular mechanisms of induction have not been deciphered. As lens is a source of FGF, we examined whether this factor was involved in inducing ciliary body. We forced the pigmented epithelium of the embryonic chick eye to express FGF4. Infected cells and their immediate neighbors were transformed into neural retina. At a distance from the FGF signal, the tissue transitioned back into pigmented epithelium. Ciliary body tissue was found in the transitioning zone. The ectopic ciliary body was never in contact with the lens tissue. In order to assess the contribution of the lens on the specification of normal ciliary body, we created optic cups in which the lens had been removed while still pre-lens ectoderm. Ciliary body tissue was identified in the anterior portion of lens-less optic cups. We propose that the ciliary body may be specified at optic vesicle stages, at the same developmental stage when the neural retina and pigmented epithelium are specified and we present a model as to how this could be accomplished through overlapping BMP and FGF signals.

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Section: Ciliary Body Tissue Is Identified At a Distance From An Fgf Sourcementioning

confidence: 71%

FGF-mediated induction of ciliary body tissue in the chick eye

Silva

Tiffin

Mima

et al. 2007

Developmental Biology

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“…Lopashov [23,24,261, on the basis of the data regarding the transdifferentiative phenomena of the eye tissues in larval and adult R a m temporaria (see previous paragraphs), formulated another interesting hypothesis, that the direction of the induced transdifferentiation could depend on the type of inducing influences, but this dependence is not unequivocal. The direction of transdifferentiation of frog iris pigmented cells (IPE) and of retinal pigmented cells (RPE) depends to some extent on directive influences from other tissues; however, this does not exclude the possibility that the multipotency of RPE an IPE can depend on the availability in them of some dormant programming determinants.…”

Section: Resultsmentioning

confidence: 99%

“…Retinal transdifferentiation of pigmented epithelium is also seen in larval and adult Rana temporaria [23, 251. In this species, retinal transdifferentiation occurs as in the case of the pigmented epithelium of Xenopus laevis. Moreover, the process can be stimulated by lens epithelium [23]. Fragments of retinal pigmented epithelium cultured in vivo, in contact with the lens epithelium in the tadpole orbit, after a period of intense proliferation, transform into retina.…”

Section: Retinal Transdifferentiation Of the Iris And Retinal Pigmentmentioning

confidence: 99%

Transdifferentiation of ocular tissues in larval Xenopus laevis

Bosco

1988

Differentiation

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“…Some investigators have recently reported cases of lensforming transdifferentiation resulting from the implantation in the enucleated orbit of retina and iris pigmented epithelium of R. temporaria precultivated in vitro in contact with the lens epithelium, while the implantation in the same area of retina pigmented epithelium together with the lens epithelium (but without precultivation) and of iris pigmented epithelium precultivated in vitro in contact with the eye vesicles leads to transdifferentiation of the pigmented epithelium cells into retina [11][12][13].…”

Section: Discussionmentioning

confidence: 99%