Transducer-Like Protein in Campylobacter jejuni With a Role in Mediating Chemotaxis to Iron and Phosphate

Chandrashekhar, Kshipra; Srivastava, Vishal; Hwang, Sunyoung; Jeon, Byeonghwa; Ryu, Sangryeol; Rajashekara, Gireesh

doi:10.3389/fmicb.2018.02674

Cited by 16 publications

(15 citation statements)

References 71 publications

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“…Motility in Campylobacter is regulated by a chemotactic signalling system that allows the organisms to follow favourable chemical gradients in environments (62). It is interesting to note that Cj0262c was up-regulated, which is the gene that encodes the transducer-like protein and is involved in chemotaxis of C. jejuni (63). Furthermore, its closely related Tlp1-3 transducer-like proteins that are also involved in the C. jejuni chemotaxis sensory system, motility, and colonisation (64) were not differentially expressed.…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Ramić

Bučar

Kunej

et al. 2021

Appl Environ Microbiol

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New approaches for the control of Campylobacter jejuni biofilms in the food industry are being studied intensively. Natural products are promising alternative antimicrobial substances to control biofilm production, with particular emphasis on plant extracts. Dried flowers of Lavandula angustifolia were used to produce LEO, LEF, and LEW. The chemical compositions determined for these Lavandula preparations included seven major compounds that were selected for further testing. These were tested against C. jejuni , for biofilm degradation and removal. Next-generation sequencing was used to study the molecular mechanisms underlying LEO actions against C. jejuni adhesion and motility. Analysis of LEO revealed 1,8-cineol, linalool and linalyl acetate as the main components. For LEF and LEW, the main components were phenolic acid glycosides, with flavonoids rarely present. The minimal inhibitory concentrations of the Lavandula preparations and pure compounds against C. jejuni ranged from 0.2 mg/mL to 1 mg/mL. LEO showed the strongest biofilm degradation. The reduction of C. jejuni adhesion was by ≥1 log 10 CFU/mL, which satisfies European Food Safety Authority recommendations. Lavandula preparations reduced C. jejuni motility by almost 50%, which consequently can impact upon biofilm formation. These data are in line with the transcriptome analysis of C. jejuni , where LEO down-regulated genes important for biofilm formation. LEW also showed good antibacterial and anti-biofilm effects, particularly against adhesion and motility mechanisms. This defines an innovative approach using alternative strategies and novel targets to combat bacterial biofilm formation, and hence the potential to develop new effective agents with biofilm-degrading activities. Importance The Lavandula preparations used in this study are found to be effective against C. jejuni , a common foodborne pathogen. They show anti-biofilm properties at sub-inhibitory concentrations in terms of promoting biofilm degradation and inhibiting cell adhesion and motility, which are involved in the initial steps of biofilm formation. These results are confirmed by transcriptome analysis, which highlights the effect of Lavandula essential oil on C. jejuni biofilm properties. We show that the waste material from the hydrodistillation of Lavandula has particular anti-biofilm effects, suggesting that it may find reuse for industrial purposes. This study highlights the need for efforts directed towards such innovative approaches and alternative strategies against biofilm formation and maintenance by developing new naturally derived agents with anti-biofilm activities.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Ramić

Bučar

Kunej

et al. 2021

Appl Environ Microbiol

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“…Though Campylobacter was first described in the 1800s, the exact mechanism by which these bacteria cause hundreds of millions of cases of diarrhea each year is largely unknown, though several virulence factors have been uncovered [2,7,38,[73][74][75]. Motility and chemotaxis are considered crucial to C. jejuni pathogenesis [30,[75][76][77][78][79]. Therefore, fully understanding the regulation of these two factors must be carefully explored and unraveled in the hopes of finding a prophylactic treatment to prevent campylobacteriosis and C. jejuni-associated sequelae.…”

Section: Discussionmentioning

confidence: 99%

The Campylobacter jejuni Response Regulator and Cyclic-Di-GMP Binding CbrR Is a Novel Regulator of Flagellar Motility

et al. 2021

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A leading cause of bacterial gastroenteritis, Campylobacter jejuni is also associated with broad sequelae, including extragastrointestinal conditions such as reactive arthritis and Guillain-Barré Syndrome (GBS). CbrR is a C. jejuni response regulator that is annotated as a diguanylate cyclase (DGC), an enzyme that catalyzes the synthesis of c-di-GMP, a universal bacterial second messenger, from GTP. In C. jejuni DRH212, we constructed an unmarked deletion mutant, cbrR−, and complemented mutant, cbrR+. Motility assays indicated a hyper-motile phenotype associated with cbrR−, whereas motility was deficient in cbrR+. The overexpression of CbrR in cbrR+ was accompanied by a reduction in expression of FlaA, the major flagellin. Biofilm assays and scanning electron microscopy demonstrated similarities between DRH212 and cbrR−; however, cbrR+ was unable to form significant biofilms. Transmission electron microscopy showed similar cell morphology between the three strains; however, cbrR+ cells lacked flagella. Differential radial capillary action of ligand assays (DRaCALA) showed that CbrR binds GTP and c-di-GMP. Liquid chromatography tandem mass spectrometry detected low levels of c-di-GMP in C. jejuni and in E. coli expressing CbrR. CbrR is therefore a negative regulator of FlaA expression and motility, a critical virulence factor in C. jejuni pathogenesis.

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“…Thus, several Gram-negative bacteria control cytoplasmic iron levels through the transcriptional regulator Fur 53 . Iron and Fur genes alter production of transducer-like proteins (tlp) that allow C. jejuni to sense environmental signals and aid bacterial invasion 54 . The Cj0145 ( phoA ) gene is located immediately downstream of tlp2 and induced in the presence of iron, likely regulating cytoplasmic iron levels 53 .…”

Section: Discussionmentioning

confidence: 99%

Campylobacter jejuni genotypes are associated with post-infection irritable bowel syndrome in humans

Peters

Pascoe

et al. 2021

Commun Biol

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Campylobacter enterocolitis may lead to post-infection irritable bowel syndrome (PI-IBS) and while some C. jejuni strains are more likely than others to cause human disease, genomic and virulence characteristics promoting PI-IBS development remain uncharacterized. We combined pangenome-wide association studies and phenotypic assays to compare C. jejuni isolates from patients who developed PI-IBS with those who did not. We show that variation in bacterial stress response (Cj0145_phoX), adhesion protein (Cj0628_CapA), and core biosynthetic pathway genes (biotin: Cj0308_bioD; purine: Cj0514_purQ; isoprenoid: Cj0894c_ispH) were associated with PI-IBS development. In vitro assays demonstrated greater adhesion, invasion, IL-8 and TNFα secretion on colonocytes with PI-IBS compared to PI-no-IBS strains. A risk-score for PI-IBS development was generated using 22 genomic markers, four of which were from Cj1631c, a putative heme oxidase gene linked to virulence. Our finding that specific Campylobacter genotypes confer greater in vitro virulence and increased risk of PI-IBS has potential to improve understanding of the complex host-pathogen interactions underlying this condition.

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Transducer-Like Protein in Campylobacter jejuni With a Role in Mediating Chemotaxis to Iron and Phosphate

Cited by 16 publications

References 71 publications

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

The Campylobacter jejuni Response Regulator and Cyclic-Di-GMP Binding CbrR Is a Novel Regulator of Flagellar Motility

Campylobacter jejuni genotypes are associated with post-infection irritable bowel syndrome in humans

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