Transducing system operated by adenosine A2A receptors to facilitate acetylcholine release in the rat hippocampus

Abstract

Although molecular biology studies indicate the presence of adenosine A 2A receptors in the rat hippocampus, the pharmacological characterization of adenosine A 2A receptor binding and of its putative facilitatory effects has revealed features essentially different from these found for adenosine A 2A receptors in most preparations. We now confirmed that activation of adenosine

Adenosine A1 receptors presynaptically modulate excitatory synaptic input onto subiculum neurons

“…In contrast, A 1 receptor density has been shown to be downregulated in the rat hippocampus following kindling (Rebola et al, 2003a) and kainic-acid (Ekonomou et al, 2000) induced seizures. Adenosine A 2A receptor density has been shown to be upregulated following seizure activity (Rebola et al, 2002). …”

Input-Specific Modulation of Neurotransmitter Release in the Lateral Horn of the Spinal Cord via Adenosine Receptors

“…Several lines of evidence converged to indicate that the primary antibody specifically detected the A2 A receptor: (1) The antibody has previously been verified as detecting the A2 A receptor in striatum and hippocampal nerve terminals (Rebola et al, 2002). (2) The antibody recognizes a protein with a molecular weight appropriate for the A2 A receptor because Western blotting of rat spinal cord resulted in a predominant band at 45 kDa with another minor band at 62 kDa ( Fig.…”

Genetic deletion of the adenosine A2A receptor prevents nicotine-induced upregulation of α7, but not α4β2* nicotinic acetylcholine receptor binding in the brain

“…Functional A 2A Rs are expressed on cholinergic nerve terminals (Preston et al, 2000;Tozzi et al, 2011), and their activation increases the release of acetylcholine (ACh) in the hippocampus (Rebola et al, 2002;Rodrigues et al, 2008) and the striatum (Gubitz et al, 1996;Kirkpatrick and Richardson, 1993). A 2A R-mediated ACh release in the rodent striatum has also been demonstrated in vivo, suggesting that striatal cholinergic neurotransmission is tonically influenced by adenosine acting on A 2A Rs .…”