Transductal Fluxes of Anions in the Rat Pancreas

Mangos, J. A.; McSherry, Nona R.; Nousia-Arvanitakis, Santa; Schilling, Robert F.

doi:10.3181/00379727-146-38097

Cited by 15 publications

(10 citation statements)

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“…The fact that intraduodenal injection of HC1 is unable to increase bicarbon ate concentration (table I) is explained by the experiments of Mangos et al (2), which showed that in rats, secretin increases the concentration of chloride and not of bicarbonate. Since the action of intraduodenal HC1 in alcoholics and in controls is not different, it is probable that the release of secretin is not greatly modified in chronic alcoholism.…”

Section: Discussionmentioning

confidence: 92%

Action of Intragastric Ethanol on the Pancreatic Secretion of Conscious Rats

Cavarzan¹,

As²,

Sarles³

et al. 1975

Digestion

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A surgical procedure has been designed which permits injection in the stomach and the duodenum by separate catheters, collection of the pancreatic juice during the experiments, recirculation of the pancreatic juice into the duodenum between experiments, and a normal circulation of bile in rats. Experiments were performed in conscious rats given either 20 % ethanol or water. In rats submitted to daily ethanol consumption for 13 months, the intragastric injection of 2 g/kg 20% ethanol considerably increased the pancreatic secretion of protein and, to a lesser extent, of water. In control non-alcoholic rats, a short period of increased secretion is followed by a major inhibition of pancreatic secretion, this reverse reaction to ethanol of pancreatic secretion according to whether or not rats are adapted to regular ethanol consumption is similar to what has been previously observed in dog. In chronic alcoholic rats, the release of secretin is probably not very different from controls.

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Section: Discussionmentioning

confidence: 92%

Action of Intragastric Ethanol on the Pancreatic Secretion of Conscious Rats

Cavarzan¹,

As²,

Sarles³

et al. 1975

Digestion

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“…In the reserpinized rat model, our findings suggest an impairment of the anion-exchange mechanism which is thought to occur in the pancreatic ducts of normal rats (9,10,13). The loss of the biphasic pattern of bicarbonate and chloride excretion after crude secretin, and the reversal in the relationship between the concentration of these two anions and flow rate after purified secretin, suggest that the exchange is either inhibited or reversed as a result of some as yet unidentified action of reserpine on the pancreatic duct cells.…”

Section: Pancreas Weights and Compositionmentioning

confidence: 92%

The Chronically Reserpinized Rat as a Possible Model for Cystic Fibrosis. VII. Alterations in the Secretory Response to Cholecystokinin and to Secretin from the Pancreas in Vivo

Perlmutter

Martinez

et al. 1978

Pediatr Res

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Summary .isoproterenol (8). In the second model, we have demonstratedThe chronically reserpinized rat has been proposed as an animal model for cystic fibrosis on the basis of morphologic and secretory alterations in the submaxillary gland and of abnormalities in pulmonary secretions. In this investigation, the volume and composition of pancreatic juice from reserpine treated rats (0.5 mg/kg/day) have been compared to those of untreated controls after stimulation with purified cholecystokinin (0.1 pg/ kg body wt) and with crude and purified preparations of secretin (6p/100 g body wt) infused iv for 30-min periods. The results demonstrate that the treated animals secreted a significantly lower volume of pancreatic juice after stimulation with these secretagogues. Fldw rates were also significantly reduced after stimulation with cholecvstokinin and crude secretin. Protein and amylase outputs in response to cholecystokinin were smaller than in control animals after unrestricted feedings, but greater after a 24-hr fast. Total bicarbonate output was also reduced after stimulation with either crude or purified secretin and the normal excretion patterns for bicarbonate and chloride were either absent or reversed in the secretin-stimulated pancreatic juice of the treated animals. Whole pancreas homogenates from the treated animals showed significant increases in Ca++ and protein content. These results indicate that chronic administration of reserpine alters the secretion of water, protein, and bicarbonate from the rat pancreas and that it affects several of the exocrine glands involved in cystic fibrosis. These findings lend support to the concept of an animal model for the human disease. SpeculationThe chronic administration of reserpine to rats has been shown to induce alterations in salivary and pulmonary secretions which resemble those seen in patients with cystic fibrosis. The concept of an animal model has been proposed on the basis of these observations. The pancreas is another exocrine gland prominently involved in cystic fibrosis and if it can be demonstrated that reserpine administration also induces alterations in pancreatic secretion, the concept of the animal model would be strengthened considerably.The generalized exocrine gland dysfunction which is characteristic of cystic fibrosis (3) has been partially reproduced in experimental animal models induced by treating rats in a chronic fashion with certain drugs that influence the level of autonomic input into these tissues. In the first of these models, changes in the excretion of monovalent ions and of basic proteins from the rat parotid gland were observed after chronic administration of that administration of reserpine for several days induces morphologic and secretory changes in the rat submaxillary gland which resemble those of cystic fibrosis (CF) (11,12). In addition, we have shown that this procedure increases the secretion of total protein and of a specific type of glycoprotein from the lungs of the treated animals, in a manner that parallels the abn...

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“…Thus, in fasted animals, the K+ concentration in samples collected at maximum secretary rates averaged 7-61 mm + 0 57 S.E. of mean (n = 8) with Boots secretin and 7-98 mM + 0-36 (n = 6) with GIH secretin, values that are significantly higher than in control samples (P < 0 001 Mangos' group (Mangos & McSherry, 1971;Mangos et al 1974). In Table 2 are listed all published values for the rat.…”

Section: Effects Of Feedingmentioning

confidence: 99%

“…Since the maximum secretary rates that we obtained in fasted rats, even using Boots secretin, were less than half those reported by Mangos (Mangos & McSherry, 1971;Mangos et al 1974), who had worked with fed animals, we have repeated our experiments with Boots secretin in fed rats. The results are summarized in Table 1.…”

Section: Effects Of Feedingmentioning

confidence: 99%

Secretion of electrolytes by the pancreas of the anaestetized rat.

Sewell¹,

Young²

1975

The Journal of Physiology

117

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SUMMARY1. HCO-, Na+ and K+ concentrations were measured in bile-free pancreatic juice collected from fasted and fed anaesthetized rats.2. Resting flow rates averaged 0-62 sal. g'1. min-' (fasted) and 2-8 /11. mi1 (fed) and the mean HCO-j concentainrseivlwe 25-8 and 33-3 mm. 3. In fasted rats, instillation of HC1 into the duodenum caused flow rate to increase threefold and HCO3 concentrations to double (66 mm).

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Transductal Fluxes of Anions in the Rat Pancreas

Cited by 15 publications

References 0 publications

Action of Intragastric Ethanol on the Pancreatic Secretion of Conscious Rats

Action of Intragastric Ethanol on the Pancreatic Secretion of Conscious Rats

The Chronically Reserpinized Rat as a Possible Model for Cystic Fibrosis. VII. Alterations in the Secretory Response to Cholecystokinin and to Secretin from the Pancreas in Vivo

Secretion of electrolytes by the pancreas of the anaestetized rat.

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