Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina

Shimmen, Teruo; Ogata, Koreaki

doi:10.1007/s10265-012-0537-z

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…This delay observed in the presence of 50 µM Wmn cannot be ascribed to the osmotic effect of the solvent because DMSO is slightly permeable across the biologic membranes (the reflection coefficient of the PM σ = 0.73–0.85). 18 According to Shimmen and Ogata 19 the difference in DMSO concentration between the intracellular and external medium disappears within 3 h after the addition of 200 mM DMSO to the outer solution. Since our measurements lasted for several hours after preliminary 2-h incubation in DMSO-containing medium, the internal hydrostatic pressure was largely equilibrated in these experiments and cannot account for the delayed pH rise.…”

Section: Resultsmentioning

confidence: 99%

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Pathways for external alkalinization in intact and in microwounded Chara cells are differentially sensitive to wortmannin

Булычев

Foissner

2017

Plant Signaling & Behavior

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Proton flows across the plant cell membranes play a major role in electrogenesis and regulation of photosynthesis and ion balance. The profiles of external pH along the illuminated internodal cells of characean algae consist of alternating high- and low-pH zones that are spatially coordinated with the distribution of photosynthetic activity of chloroplasts underlying these zones. The results based on confocal laser scanning fluorescence microscopy, pH microsensors, and pulse-amplitude-modulated chlorophyll microfluorometry revealed that the coordination of H+ transport and photosynthesis is disrupted by the 2 different environmental cues (low light and wounding) and by a chemical, wortmannin interfering with the inositol phospholipid metabolism. On the one hand, the transition from moderate to low irradiance diminished the peaks in the profiles of photosystem II (PSII) quantum efficiency but did not remove the pH bands. On the other hand, the microwounding of the internode with a glass micropipette, impacting primarily the cell wall, resulted in a rapid local alkalinization of the external medium (by 2–2.5 pH units) near the cell surface, thus mimicking the appearance of natural pH bands. Despite their seeming similarity, the alkaline bands of intact cells were eliminated by wortmannin, whereas the wound-induced alkalinization was insensitive to this drug. Furthermore, the attenuation of natural pH bands in wortmannin-treated cells was accompanied by the enhancement in spatial heterogeneity of PSII efficiency and electron transport rates, which indicates the complexity of chloroplast–plasma membrane interactions. The results suggest that the light- and wound-induced alkaline areas on the cell surface are associated with different ion-transport systems.

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Section: Resultsmentioning

confidence: 99%

“…This solvent at a concentration of 1–2% had no influence on cytoplasmic streaming and the membrane potential of characean cells. 19,28 …”

Section: Methodsmentioning

confidence: 99%

Pathways for external alkalinization in intact and in microwounded Chara cells are differentially sensitive to wortmannin

Булычев

Foissner

2017

Plant Signaling & Behavior

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Long-range interactions of Chara chloroplasts are sensitive to plasma-membrane H+ flows and comprise separate photo- and dark-operated pathways

Булычев

Rybina

2018

Protoplasma

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Local illumination of the characean internode with a 30-s pulse of white light was found to induce the delayed transient increase of modulated chlorophyll fluorescence in shaded cell parts, provided the analyzed region is located downstream in the cytoplasmic flow at millimeter distances from the light spot. The fluorescence response to photostimulation of a remote cell region indicates that the metabolites produced by source chloroplasts in an illuminated region are carried downstream with the cytoplasmic flow, thus ensuring long-distance communications between anchored plastids in giant internodal cells. The properties of individual stages of metabolite signaling are not yet well known. We show here that the export of assimilates and/or reducing equivalents from the source chloroplasts into the flowing cytoplasm is largely insensitive to the direction of plasma-membrane H flows, whereas the events in sink regions where these metabolites are delivered to the acceptor chloroplasts under dim light are controlled by H fluxes across the plasma membrane. The fluorescence response to local illumination of remote cell regions was best pronounced under weak background light and was also observed in a modified form within 1-2 min after the transfer of cell to darkness. The fluorescence transients in darkened cells were suppressed by antimycin A, an inhibitor of electron transfer from ferredoxin to plastoquinone, whereas the fluorescence response under background light was insensitive to this inhibitor. We conclude that the accumulation of reduced metabolites in the stroma leads to the reduction of photosystem II primary quinone acceptor (Q) via two separate (photochemical and non-photochemical) pathways.

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Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina

Cited by 2 publications

References 30 publications

Pathways for external alkalinization in intact and in microwounded Chara cells are differentially sensitive to wortmannin

Pathways for external alkalinization in intact and in microwounded Chara cells are differentially sensitive to wortmannin

Long-range interactions of Chara chloroplasts are sensitive to plasma-membrane H+ flows and comprise separate photo- and dark-operated pathways

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