Transduction of Redox Signaling by Electrophile-Protein Reactions

Rudolph, Tanja K.; Freeman, Bruce Α.

doi:10.1126/scisignal.290re7

Cited by 195 publications

(206 citation statements)

References 148 publications

(132 reference statements)

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“…The thiol of cysteine is one such target that can be oxidized by the ROS, 40 and lipid peroxidation products can also give rise to thiol oxidized products. 41 In the present study, it was found that nanoparticles with no or low concentration of dopants were causing more oxidation of the protein thiols compared to those with higher dopant concentration, that is, DNP2 and DNP3. DNP4 was responsible for the highest quantity of thiol oxidation products (102 nM) while DNP2 and DNP3 were producing ,40 nM (Figure 9).…”

Section: Protein Thiolsmentioning

confidence: 73%

Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation

Nadhman¹,

Khan²,

Nazir³

et al. 2016

IJN

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Lipid and protein oxidation are well-known manifestations of free radical activity and oxidative stress. The current study investigated extermination of Leishmania tropica promastigotes induced by lipid and protein oxidation with reactive oxygen species produced by PEGylated metal-based nanoparticles. The synthesized photodynamic therapy-based doped and nondoped zinc oxide nanoparticles were activated in daylight that produced reactive oxygen species in the immediate environment. Lipid and protein oxidation did not occur in dark. The major lipid peroxidation derivatives comprised of conjugated dienes, lipid hydroperoxides, and malondialdehyde whereas water, ethane, methanol, and ethanol were found as the end products. Proteins were oxidized to carbonyls, hydroperoxides, and thiol degrading products. Interestingly, lipid hydroperoxides were produced by more than twofold of the protein hydroperoxides, indicating higher degradation of lipids compared to proteins. The in vitro evidence represented a significant contribution of the involvement of both lipid and protein oxidation in the annihilated antipromastigote effect of nanoparticles.

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Section: Protein Thiolsmentioning

confidence: 73%

Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation

Nadhman¹,

Khan²,

Nazir³

et al. 2016

IJN

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“…Our understanding of the role of ROS in normal physiological signaling is evolving rapidly (3,33,36,37). The present studies define key roles for endogenous H 2 O 2 in cardiac myocytes that modulate Ang-II signaling pathways controlling eNOS activation and myocyte contractility.…”

Section: Resultsmentioning

confidence: 88%

“…However, not all effects of ROS are deleterious. Endogenously generated ROS have been implicated in posttranslational protein modifications that subserve critical roles in cellular signaling (3). Hydrogen peroxide (H 2 O 2 ) is one such ROS that has recently been identified as a key physiological signaling molecule in many cell types (4,5).…”

mentioning

confidence: 99%

Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis

Sartoretto

Kalwa

Pluth

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Nitric oxide (NO) and hydrogen peroxide (H 2 O 2 ) are synthesized within cardiac myocytes and play key roles in modulating cardiovascular signaling. Cardiac myocytes contain both the endothelial (eNOS) and neuronal (nNOS) NO synthases, but the differential roles of these NOS isoforms and the interplay of reactive oxygen species and reactive nitrogen species in cardiac signaling pathways are poorly understood. Using a recently developed NO chemical sensor [Cu 2 ðFL2EÞ] to study adult cardiac myocytes from wild-type, eNOS null , and nNOS null mice, we discovered that physiological concentrations of H 2 O 2 activate eNOS but not nNOS. H 2 O 2 -stimulated eNOS activation depends on phosphorylation of both the AMPactivated protein kinase and kinase Akt, and leads to the robust phosphorylation of eNOS. Cardiac myocytes isolated from mice infected with lentivirus expressing the recently developed H 2 O 2 biosensor HyPer2 show marked H 2 O 2 synthesis when stimulated by angiotensin II, but not following β-adrenergic receptor activation. We discovered that the angiotensin-II-promoted increase in cardiac myocyte contractility is dependent on H 2 O 2 , whereas β-adrenergic contractile responses occur independently of H 2 O 2 signaling. These studies establish differential roles for H 2 O 2 in control of cardiac contractility and receptor-dependent NOS activation in the heart, and they identify new points for modulation of NO signaling responses by oxidant stress.

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“…Urinary Kelch-like ECH-associated protein 1 (Keap1) regulator of nuclear factor (erythroid-derived-2)-like 2 (Nrf2), heat shock factor-1 (HSF-1), peroxisome proliferator-activated receptor-␥ (PPAR ␥ ), and histone deacetylases (HDAC) ( 7 ). These transcriptional regulatory proteins contribute to the control of the expression of hundreds of genes that include cytokines, antioxidant enzymes, heat shock response proteins, and enzymes of intermediary metabolism ( 4,5,(8)(9)(10)(11)(12)(13)(14).…”

Section: Lipid Extraction From Urinementioning

confidence: 99%

Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine

Salvatore

Vitturi

Baker

et al. 2013

Journal of Lipid Research

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This article is available online at http://www.jlr.org biomolecule nitration ( 1 ). Nitroalkene substituents are electrophilic and promote Michael addition of fatty acids with biological nucleophiles such as cysteine and histidine. The extent, rate, and reversibility of these reactions will be dictated both by the concentration and reactivity of individual nucleophiles. In this regard, protein structure and compartmentalization affect the reactivity of individual nucleophilic centers and will defi ne the molecular targets of electrophilic fatty acids.While enzymatically-oxygenated unsaturated fatty acids typically transduce anti-infl ammatory actions via specifi c g protein-coupled receptor ligand activity ( 2, 3 ), transcriptional responses to electrophilic fatty acids reveal that a broader array of signaling events are instigated ( 4, 5 ). The basis for this pleiotropy resides in the facile Michael addition of electrophilic fatty acid derivatives with nucleophilic centers of proteins that regulate structure and function ( 6 ). Functionally-signifi cant protein targets of electrophilic fatty acids include the transcriptional regulatory protein complex nuclear factor kappa B (NFkB), the

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Transduction of Redox Signaling by Electrophile-Protein Reactions

Cited by 195 publications

References 148 publications

Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation

Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation

Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis

Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine

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