Transductional Analysis of Cysteine Mutants in Proteus mirabilis

Grabow, W. O. K.

doi:10.1099/00221287-70-1-77

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…The addition of Cys unexpectedly promotes proliferation of i‐PM in the suspension with RP, resulting in color changing from red to gray, probably because an auxotrophic mutation in association with synthetic routes of Cys via transductive enzymatic reactions in i‐PM might be occurred by coculturing with RP. [ 24 ]…”

Section: Resultsmentioning

confidence: 99%

“…The addition of Cys unexpectedly promotes proliferation of i-PM in the suspension with RP, resulting in color changing from red to gray, probably because an auxotrophic mutation in association with synthetic routes of Cys via transductive enzymatic reactions in i-PM might be occurred by coculturing with RP. [24] The solution of bacteria in the ATCC 543 medium without Cys turned red after subculturing and formed red and white colonies on an ATCC 543 agar plate with SD and without Cys, respectively. Although SD restraints bacterial swarming of PM, indistinct and translucent colonies of PM intermingle with RP colonies, making it difficult to sort a single colony of RP.…”

Section: Isolation Of Highly Effective Anticancer Bacteria From Tumorsmentioning

confidence: 99%

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Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics

et al. 2023

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Unveiling biomedical functions of tumor‐resident microbiota is challenging for developing advanced anticancer medicines. This study demonstrates that isolated intratumoral bacteria, associated with natural purple photosynthetic bacteria, have inherent biocompatibility and strong immunogenic anticancer efficacies. They preferentially grow and proliferate within a targeted tumor milieu, which effectively causes immune cells to infiltrate the tumor and provoke strong anticancer responses in various syngeneic mouse models, including colorectal cancer, sarcoma, metastatic lung cancer, and extensive drug‐resistant breast cancer. Furthermore, these functional bacteria‐treated mice exhibit excellent anticancerous responses and have significantly prolonged survival rates with effective immunological memory. Light‐harvesting nanocomplexes of microbial consortia of intratumoral bacteria and purple photosynthetic bacteria can diagnose tumors using bio‐optical‐window near‐infrared light, making them useful theranostic agents for highly targeted immunological elimination of the tumor and for precisely marking tumor location.

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Section: Resultsmentioning

confidence: 99%

Section: Isolation Of Highly Effective Anticancer Bacteria From Tumorsmentioning

confidence: 99%

Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics

et al. 2023

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Chromosome Transfer in Proteus Mirabilis Mediated by a Hybrid Plasmid

Coetzee

1975

Journal of General Microbiology

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A previously-described fused plasmid, P-ZacR 1drd19, was found to mediate chromosomal transfer between cells of Proteus mirabilis strain ~~5 0 0 6; ~~5 0 0 6 -(P-ZacRrdrd19) was usually the donor and various auxotrophs of ~~5 0 0 6 resistant to nalidixic acid and/or streptomycin were recipients. The donor was usually counterselected with nalidixic acid and/or high concentrgtions of streptomycin. Recombination experiments with single markers indicated a 40-fold variation in recombination frequencies for different markers. Mapping double-auxotrophic markers by their gradient of transmission confirmed this variation and placed each of two independent isolates of eight markers in a linkage group his-ser-ura-pyrBtrp-cys-ade-ilv. Some donor markers did not register. Despite low recombination frequencies, interrupted mating experiments showed a polarity of early marker transfer. The segregation of unselected markers confirmed the order of some markers and showed that genetic material passed from the presumptive donor to the recipient. Recipients with two auxotrophic markers which could not be cotransduced by phage 5006M were converted to prototrophy by conjugation. The plasmid transferred to recipients at high frequency and all recombinants carried it. Recombinants could act as donors in further matings. Recombinants were fully susceptible to phage 5006M, unlike transductants of ~~5 0 0 6 by this phage. Direct involvement of the plasmid was indicated by drastically diminished recombination frequencies in crosses with recipients carrying P-lac as resident. P-lac had previously been shown to reduce the frequency of transfer of the hybrid plasmid to cells harbouring it. The histidine region was the first to register in recipients and recombined at the highest frequency of 5 x Io-6/donor cell. Some temporary association of plasmid and perhaps only the histidine region of the chromosome is favoured as the mechanism of chromosomal transfer. This could explain why not all donor markers could be mapped. Transduction and transformation were excluded as the cause of results.

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Transductional Analysis of Cysteine Mutants in Proteus mirabilis

Cited by 2 publications

References 38 publications

Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics

Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics

Chromosome Transfer in Proteus Mirabilis Mediated by a Hybrid Plasmid

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