Transepithelial Transport Route and Liposome Encapsulation of Milk-Derived ACE-Inhibitory Peptide Arg-Leu-Ser-Phe-Asn-Pro

Zhang, Tao; Su, M.; Jiang, Xiaoxiao; Xue, Yiqiu; Zhang, Jiaxin; Zeng, Xiaoqun; Wu, Zhen; Guo, Yuxing; Pan, Daodong

doi:10.1021/acs.jafc.9b00397

Cited by 44 publications

(42 citation statements)

References 37 publications

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“…Thus, high amount of the peptides with DPPH radical scavenging activity or EGCG could not diffuse on the basolateral side. Generally, there are three main pathways for the intestinal transport of bioactive peptides, involving PepT1 route, paracellular, and transcytosis pathways (Zhang et al., 2019). Di/tripeptides can be transported by the PepT1 route.…”

Section: Resultsmentioning

confidence: 99%

“…Apart from PepT1‐mediated transport, low water‐soluble oligopeptides or EGCG are mainly transported via the paracellular route, which is controlled by the tight junctions of enterocytes. Furthermore, lipophilic peptides with large molecules could interact with the lipid bilayer of the cell membrane via hydrophobic interaction (Zhang et al., 2019). Zhao et al.…”

Section: Resultsmentioning

confidence: 99%

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Effects of sonication and ultrasound on properties and bioactivities of liposomes loaded with hydrolyzed collagen from defatted sea bass skin conjugated with epigallocatechin gallate

et al. 2021

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Hydrolyzed collagen (HC) from defatted sea bass skin conjugated with 3% epigallocatechin gallate (EGCG) was prepared and the resulting HC–EGCG conjugate at various levels (0.25%–2%, w/v) was loaded into liposome. The obtained liposomes were subjected to sonication (S). Liposome loaded with 1% conjugate showed the highest encapsulation efficiency (EE) (p < .05). When the ultrasound‐assisted process (UAP) at different amplitudes (20% and 40%) and times (2, 5, 10, and 15 min) were implemented, the highest EE of conjugate‐loaded liposome was found at 20% amplitude for 2 min (p < .05). When S‐liposome and UAP‐liposome were lyophilized, decreasing EE of both samples was observed (p < .05). Lyophilized UAP‐liposome had higher stability than lyophilized S‐liposome during storage at 25℃ for 28 days. Additionally, antioxidant activity in the gastrointestinal track model system (GIMs) and digest obtained from GIMs were higher for UAP‐liposome (p < .05). Therefore, liposome can be used for the delivery of conjugate. Practical applications HC from defatted sea bass skin is considered to possess several bioactivities, especially skin nourishment and bone strengthening. Nevertheless, antioxidant activity, related to the treatment of several ailments, is still low for HC. Thus, grafting of HC with polyphenol such as EGCG via free radical method can be used for the enhancement of the antioxidant activity of HC. Although the resulting conjugate has augmented activity, it is unstable during storage and in the gastrointestinal digestion system. Liposome is a promising means to stabilize the conjugate under harsh condition, especially with the aid of the UAP. Thus, liposome loaded with conjugate having the reduced size has higher antioxidant activity with increased stability, which can have a wider range of applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effects of sonication and ultrasound on properties and bioactivities of liposomes loaded with hydrolyzed collagen from defatted sea bass skin conjugated with epigallocatechin gallate

et al. 2021

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“…Hence, the affinity between peptides and phospholipid-rich cell membrane could increase with peptide hydrophobicity (Wang et al, 2019). For instance, bradykinin (RPPGFSPFR) and RLSFNP have been proved transported mainly via the transcytosis pathway (Vij, Reddi, Kapila, & Kapila, 2016;Zhang et al, 2019). The transport route for different peptides depends on several factors, such as hydrophobicity, molecular size, and charge (Vermeirssen et al, 2004;Wang & Li, 2018).…”

Section: Transcytosismentioning

confidence: 99%

Activity and bioavailability of food protein‐derived angiotensin‐I‐converting enzyme–inhibitory peptides

Xue

Yin

Howell

et al. 2021

Comp Rev Food Sci Food Safe

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Angiotensin‐I‐converting enzyme (ACE) inhibitory peptides are able to inhibit the activity of ACE, which is the key enzymatic factor mediating systemic hypertension. ACE‐inhibitory peptides can be obtained from edible proteins and have the function of antihypertension. The amino acid sequences and the secondary structures of ACE‐inhibitory peptides determine the inhibitory activities and stability. The resistance of ACE‐inhibitory peptides to digestive enzymes and peptidase affect their antihypertensive bioactivity in vivo. In this paper, the mechanism of ACE‐inhibition, sources of the inhibitory peptides, structure–activity relationships, stability during digestion, absorption and transportation of ACE‐inhibitory peptides, and consumption of ACE‐inhibitory peptides are reviewed, which provide guidance to the development of new functional foods and production of antihypertensive nutraceuticals and pharmaceuticals.

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“…However, major destructive effects were not observed by using bacitracin in rat intestine [100]. Besides, encapsulation of RLSFNP by liposome could also facilitate the intact peptide transportation through transcytosis in Caco2 cell [101]. In addition, Li et al [102] used nano-encapsulation of antidiabetic peptides made by chitosan coated liposome to maintain the stability of peptides.…”

Section: Bioavailabilitymentioning

confidence: 99%

Bioactive Peptides from Agriculture and Food Industry Co-Products: Peptide Structure and Health Benefits

Yongsawatdigul¹,

Hamzeh²

2021

Innovation in the Food Sector Through the Valorization of Food and Agro-Food by-Products

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Co-products from food processing are typically disposed or turned into low value animal feed. Proteinaceous co-products can be converted to bioactive peptides exerting health benefits, which can lead to development of nutraceuticals and functional foods. This is an effective means for valorization of these co-products. The release of encrypted peptides exhibits various bioactivities, including antihypertension, antioxidant, immunomodulatory activities among others, in vitro, and some activities have been demonstrated in vivo. Structure modification of bioactive peptides occurring under gastrointestinal digestion and cellular transport remains the important factor determining the health benefits of bioactive peptides. Understanding peptide transformation in gastrointestinal tract and in blood circulation before reaching the target organs would shed some lights on its bioavailability and subsequently ability to exert physiological impact. In this chapter, the potential health promoting properties of peptides encrypted in various sources of co-products will be reviewed based on evidence on in vitro, in vivo and clinical trial studies. Structural changes of bioactive peptides under physiological condition will also be discussed in relation to its bioactivities.

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Transepithelial Transport Route and Liposome Encapsulation of Milk-Derived ACE-Inhibitory Peptide Arg-Leu-Ser-Phe-Asn-Pro

Cited by 44 publications

References 37 publications

Effects of sonication and ultrasound on properties and bioactivities of liposomes loaded with hydrolyzed collagen from defatted sea bass skin conjugated with epigallocatechin gallate

Effects of sonication and ultrasound on properties and bioactivities of liposomes loaded with hydrolyzed collagen from defatted sea bass skin conjugated with epigallocatechin gallate

Activity and bioavailability of food protein‐derived angiotensin‐I‐converting enzyme–inhibitory peptides

Bioactive Peptides from Agriculture and Food Industry Co-Products: Peptide Structure and Health Benefits

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