Transesterification of fish oil by lipase immobilized in supercritical carbon dioxide

Roh, Myong-Kyun; Choi, Jae‐Suk

doi:10.1007/s12562-015-0926-z

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…In the literature, different immobilized lipases have been used to successfully conduct alcoholysis reactions in scCO2, such as Lipozyme RM IM from Rhizomucor miehei [84,115,116], Lipozyme/Novozyme 435 from Candida antarctica [117][118][119][120][121][122], and Lipozyme TL-IM from Thermomuces lanuginose [123,124]. Marty et al [115] compared the esterification of oleic acid and ethanol in scCO2 and in hexane by an immobilized lipase from R. miehei (Lipozyme), finding higher initial reaction rates and lower ethanol inhibition in scCO2.…”

Section: Fish Oil Transesterification Kinetics In Scco2mentioning

confidence: 99%

“…The reaction products, fatty acid ethyl esters (FAEEs) were continuously extracted by the flowing supercritical phase; thus, CO2 flowrate and phase behavior of the system influenced the reaction rate and product composition [118][119][120]. Some years later, the transesterification of menhaden oil with excess ethanol in scCO2 was J o u r n a l P r e -p r o o f investigated [123,124], finding that reaction rates were enhanced and ethanol inhibition was reduced in scCO2 when compared to organic solvent or solvent-free systems. An empirical kinetic model assuming reversible binary reactions at each transesterification step was proposed [124], obtaining good fitting results.…”

Section: Fish Oil Transesterification Kinetics In Scco2mentioning

confidence: 99%

“…Some years later, the transesterification of menhaden oil with excess ethanol in scCO2 was J o u r n a l P r e -p r o o f investigated [123,124], finding that reaction rates were enhanced and ethanol inhibition was reduced in scCO2 when compared to organic solvent or solvent-free systems. An empirical kinetic model assuming reversible binary reactions at each transesterification step was proposed [124], obtaining good fitting results. The concentrations of all the species in the reaction system were considered in the model, except for glycerol due to the sn-1,3 specificity of the biocatalyst.…”

Section: Fish Oil Transesterification Kinetics In Scco2mentioning

confidence: 99%

“…Our research group has presented some kinetic results for the enzymatic ethanolysis of refined fish oil from tuna and sardine in scCO2, using Lipozyme RM IM as catalyst [84]. Reaction kinetics were correlated to an empirical kinetic model based on the mass-balance of each compound (TAG, ethanol, DAG, MAG, FAEE and glycerol), similar to the one proposed in [124]. The effect of temperature (50-80 ºC) on the kinetic rate constants was considered by introducing an Arrhenius-type dependence in the kinetic model, whereas the volume and energy of activation of the reaction accounted for the effect of pressure (7.5-30 MPa).…”

Section: Fish Oil Transesterification Kinetics In Scco2mentioning

confidence: 99%

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Supercritical CO2 processing of omega-3 polyunsaturated fatty acids – Towards a biorefinery for fish waste valorization

Melgosa

Sanz

Beltrán

2021

The Journal of Supercritical Fluids

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Section: Fish Oil Transesterification Kinetics In Scco2mentioning

confidence: 99%

Section: Fish Oil Transesterification Kinetics In Scco2mentioning

confidence: 99%

Section: Fish Oil Transesterification Kinetics In Scco2mentioning

confidence: 99%

Section: Fish Oil Transesterification Kinetics In Scco2mentioning

confidence: 99%

See 2 more Smart Citations

Supercritical CO2 processing of omega-3 polyunsaturated fatty acids – Towards a biorefinery for fish waste valorization

Melgosa

Sanz

Beltrán

2021

The Journal of Supercritical Fluids

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“…SC-CO 2 has liquid-like density but gas-like viscosity resulting in high mass transfer being a clean alternative to replace organic solvents. Enzymatic concentration of n-3 PUFA in supercritical fluids (SCFs) is an interesting option for the prevention of oxidation during processing of fish oil (Lin et al 2006;Roh et al 2015). Besides, SC-CO 2 can be easily separated from the reaction products by simple depressurization and allows fractionation of the reaction products.…”

Section: Introductionmentioning

confidence: 99%

Production of omega-3 fatty acids acylglycerides by lipase-catalyzed glycerolysis of sardine oil in different reaction media

Solaesa¹

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Production of omega-3 polyunsaturated fatty acids (n-3 PUFA) concentrates is of great interest for both the pharmaceutical and the food industries. It is well known that the main n-3 PUFA, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, have beneficial effects on the prevention and reduction of cardiovascular and inflammatory diseases. However, not all the chemical forms of n-3 PUFA are equal. The bioavailability and susceptibility to oxidation of n-3 PUFA concentrates depend on their molecular structure. Monoacylglycerides and diacylglycerides (MAG and DAG) containing n-3 PUFA present good bioavailability and oxidation stability. Moreover, they present excellent emulsifying properties, being widely used in the food, cosmetic and pharmaceutical industries. Taking into account these considerations and the advantages of enzymatic technology in the formation of this kind of products, this Thesis is focused on the production of MAG rich in n-3 PUFA by a lipase-catalyzed glycerolysis reaction in different reaction systems. The main drawback of this process is the immiscibility of the substrates, therefore the main Además del estudio de la glicerolisis enzimática, otro objetivo de esta Tesis, fue la optimización del proceso de destilación molecular para fraccionar los productos de reacción y concentrar los MAG ricos en AGPI n-3. Se han evaluado diferentes temperaturas de evaporación para maximizar la recuperación de los MAG con alta pureza. También se ha evaluado en esta tesis la oxidación lípidica del aceite de sardina y de los productos de reacción. En este sentido se ha demostrado que las lipasas inmovilizadas son capaces de adsorber los productos de oxidación, tanto primarios como secundarios, formados durante la reacción de glicerolisis. (Psota et al. 2006). Excessive amounts of n-6 PUFA and hence a very high n-6/n-3 ratio of about around 15-20/1, is found in today's Western diets mostly derived from the high consumption of vegetable oils, as such or added in almost every commercially prepared food. A lower ratio of roughly four parts n-6 PUFA to one part n-3 PUFA is more desirable in reducing the risk of many of the chronic diseases of high prevalence in Western The most important natural sources of n-3 PUFA are marine organisms; this is fish, seafood and algae. EPA and DHA are synthetized by marine phytoplankton that feeds, directly or indirectly, these marine organisms. Herring, mackerel, salmon, sardines, tuna, and krill have a fairly good quantity of these compounds (Table I.1). In general, the greasier the fish the greater their concentration of EPA and DHA. However, some species of fish may contain significant levels of toxic compounds such as methylmercury, polychlorinated biphenyls (PCBs), dioxins, and other environmental contaminants. These substances are present at low levels in fresh waters and oceans, but they bioconcentrate in the aquatic food chain reaching higher values in old, large, predatory fish and marine mammals. Therefore, PCBs and methylmercury have long halflives in the body and ca...

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Progress in Supercritical Fluid Technology for Fats and Oils Processing

Temelli

Güçlü‐Üstündağ

Couto

2020

Bailey's Industrial Oil and Fat Products

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Supercritical CO 2 (SC‐CO 2 ) processing of fats and oils has been widely investigated since SC‐CO 2 offers an environmentally friendly alternative with added advantages such as moderate operating conditions and solvent‐free extracts and residues. From a processing perspective, a unique advantage of SC‐CO 2 processing lies in its versatility, which results from the ability to modify solvent properties by changing operating conditions (temperature and pressure) or by the addition of cosolvents. A good understanding of the fundamentals of solubility behavior of lipid components in SC‐CO 2 as affected by operating conditions and solute properties is required to realize its full potential in fats and oils processing. The operational flexibility offered by supercritical fluid technology enables the processor to fine‐tune solvent properties and to develop novel processes by the integration of unit operations of extraction, fractionation, reaction, and particle formation to meet the process objectives. These unit operations have been effectively utilized for the extraction and refining of oils, concentration of bioactive components from oils or oil by‐products, modification of the physical properties of fats and oils, production of oleochemicals and design of delivery systems for numerous applications.

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Transesterification of fish oil by lipase immobilized in supercritical carbon dioxide

Cited by 9 publications

References 27 publications

Supercritical CO2 processing of omega-3 polyunsaturated fatty acids – Towards a biorefinery for fish waste valorization

Supercritical CO2 processing of omega-3 polyunsaturated fatty acids – Towards a biorefinery for fish waste valorization

Production of omega-3 fatty acids acylglycerides by lipase-catalyzed glycerolysis of sardine oil in different reaction media

Progress in Supercritical Fluid Technology for Fats and Oils Processing

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