Transfection and Stable Transformation of Adult Mouse Schwann Cells with SV-40 Large T Antigen Gene

Establishment of immortalized Schwann cells from Sandhoff mice and corrective effect of recombinant human β-hexosaminidase A on the accumulated GM2 ganglioside

“…Primary and long-term cultures of DRG and adjacent peripheral nerves collected from 8-week-old Sandhoff mice were performed and spontaneously immortalized Schwann cells were obtained, as previously described (Watabe et al 1990(Watabe et al , 1994(Watabe et al , 1995(Watabe et al , 2001. Among them, an established Schwann cell line from a Sandhoff mouse (1113C1) was cultured in Iscove's modified Dulbecco's minimum essential medium supplemented with 5% fetal calf serum (FCS), 50 units/ml penicillin, and 50 lg/ml streptomycin at 37°C under a 5% CO 2 -95% air mixture.…”

A spontaneously immortalized Schwann cell line to study the molecular aspects of metachromatic leukodystrophy

“…Although the detailed mechanism is still unknown, it is likely that the spontaneous immortalisation of long term cultured Schwann cells is a general phenomenon in both wild type and mutated mice (Watabe et al, 2003). To date more than 15 Schwann cell lines have been established by spontaneous immortalization or transfection with oncogenes (Bolin et al, 1992;Boutry et al, 1992;Chen et al, 1987;Goda et al, 1991;Jirsova et al, 1997;Li et al, 1996;Ridley et al, 1988;Tennekoon et al, 1987;Thi et al, 1998;Watabe et al, 1990Watabe et al, , 1995. The degree of phenotype expression and differentiation differ from each other (Hai et al, 2002).…”

Establishment of immortalized Schwann cells from Fabry mice and their low uptake of recombinant α-galactosidase

“…Primary and long-term cultures of dorsal root ganglia and adjacent peripheral nerves derived from Fabry mice were performed according to the method previously described (Watabe et al 1990(Watabe et al , 1995. After 6 months in culture, spontaneously emerging colonies were isolated using cloning rings and expanded further.…”