Transfection efficiencies of PAMAM dendrimers correlate inversely with their hydrophobicity

Non-virally Modified Human Mesenchymal Stem Cells Produce Ciliary Neurotrophic Factor in Biodegradable Fibrin-Based 3D Scaffolds

“…3,12 Ethidium bromide-based biophysical analysis of dendrimer-plasmid complexation is built on the intercalation of this fluorescent dye into the doublestranded pCNTF vector. EB occupies an effective binding site for two to four base pairs, increasing significantly its fluorescence intensity and causing a blue shift in its maximum 8 max em .…”

“…After the overnight exposure to DNA-dendrimer complexes, hMSCs were harvested by trypsinization and embedded into fibrin scaffolds as described earlier. 13 HEK 293T transfections were performed as described 3 to compare cytotoxic and genotoxic effects of dendrimer. hrGFP fluorescence was monitored by microscopy and the percentage of GFP-positive cells was determined using a FACScan analytical flow cytometer (Becton, Dickinson and Company) after fixation with 2% paraformaldehyde.…”

“…3,15 A variety of dendrimers were tested earlier for transfection properties and cytotoxicity on HEK 293 line and human MSCs, with PAMAM-NH 2 G4 found most effective. 3 At the next stage, pCNTF vector complexation with PAMAM-NH2 G4 dendrimer was characterized biophysically by EB and ANS assays.…”

“…Biosafety considerations appeal to the use of nonviral carriers, which include polycations, lipophilic compounds, magnetic particles, dendrimers, and so on. [3][4][5] Dendrimers are macromolecules characterized by highly branched three-dimensional (3D) structure, providing a high degree of surface functionality and versatility. Their branching architecture, multivalency, and well-defined size and molecular weight clearly distinguish these nanoparticles as unique carriers in biomedical applications such as gene transfection, drug delivery, cell culture, and so on.…”

Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency

“…A transfection reagent called SuperFect™ consisting of activated dendrimers is commercially available (21). Transfection efficiency mediated by PAMAM dendrimers appears to be dependent on dendrimer generation, with larger sizes providing higher efficiency, as well as on the charge ratio of the complexes, in which a net positive charge is optimal and inversely related to their hydrophobicity (22).…”