Transfection of cells in suspension by ultrasound cavitation

Reslan, Lina; Mestas, Jean‐Louis; Herveau, Stéphanie; Béra, Jean‐Christophe; Dumontet, Charles

doi:10.1016/j.jconrel.2009.10.029

Cited by 42 publications

(27 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The other was by monitoring for acoustic harmonic emissions associated with cavitating bubbles [30,33]. Both these techniques indicated that indeed cavitation was occurring, on which the process of sonoporation is based [13,14,52,53]. The intensity threshold for consistently obtaining cavitation in free field (i.e.…”

Section: Discussionmentioning

confidence: 99%

Development of a custom biological scaffold for investigating ultrasound-mediated intracellular delivery

Bui

Aleid

Alassaf

et al. 2017

Materials Science and Engineering: C

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Development of a custom biological scaffold for investigating ultrasound-mediated intracellular delivery

Bui

Aleid

Alassaf

et al. 2017

Materials Science and Engineering: C

View full text Add to dashboard Cite

“…Human follicular lymphoma cells (RL), human acute p r o m y e l o c y t i c l e u k e m i a c e l l ( H L -6 0 ) , h u m a n erythroleukemic cells (K562), human mantle cell lymphoma cells (GRANTA), and human acute lymphoblastic leukemia (ALL) cells (CCRF-CEM) were transfected with pSuperior.neo-cN-II (pScN-II), pSuperior.neo-control (pScont), pcDNA3-GFP (pcGFP), pcDNA3-GFP-cN-II (pccN-II), or pcDNA3-GFP-cN-II-R367Q (pcR367Q) using a sonoporation method as previously described [18]. Briefly, cells (300,000 cells per tube) in a 2-mL Eppendorf tube were subjected to sonoporation (100 Hz, −1.8 dbm, 20 s) in the presence of 10 μg of plasmid.…”

Section: Plasmid Construction and Transfectionmentioning

confidence: 99%

Determination of the enzymatic activity of cytosolic 5′-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models

et al. 2015

Self Cite

View full text Add to dashboard Cite

The cytosolic 5'-nucleotidase (cN-II) has been shown to be involved in the response of cancer cells to cytotoxic agents, and the quantification of its activity in biological samples is of great interest. In this context, we developed and validated an analytical method for determination of cN-II activity in cultured cancer cells. This non-radioactive method, using a Hypercarb column as stationary phase, was validated with a lower limit of quantification of 0.1 μM inosine. We used it to characterize cell line models with modified cN-II expression obtained with stable transfections. We show that the short hairpin RNA (shRNA)-mediated inhibition of cN-II expression in various malignant blood cells is associated with decreased protein expression and enzymatic activity (1.7-6.2-fold) as well as an increased sensitivity to cytotoxic agents (up to 14-fold). On the other hand, expression of green fluorescent protein (GFP)-fused wild type or hyperactive mutant (R367Q) cN-II increased the activity and also decreased the sensitivity to nucleoside analogues. Our results confirm the biological relevance of modulating cN-II in cancer cells, and we present a straightforward validated method for the determination of cN-II activity in cellular samples.

show abstract

“…It is generally believed that ultrasound cavitation effect is produced by the following three different mechanisms [9], including (1) producing transient pores on vascular endothelial cell surface to promote macromolecular absorption into the cells; (2) destroying the integrity of the vascular endothelium to make large molecules transfer through the intercellular transfer; and (3) stimulating cell endocytosis function to enhance the intracellular delivery [10]. Sonoporation is described as the phenomenon of forming temporary small holes on cell membrane after ultrasound exposure, which can promote the uptake of extracellular substance into the cell [11, 12]. Numerous studies have showed that sonoporation effect was related to microstreaming, microjet, and shock wave, especially the inertial cavitation, after ultrasound exposure.…”

Section: The Proposed Mechanism Of Utmdmentioning

confidence: 99%

New Progress in Angiogenesis Therapy of Cardiovascular Disease by Ultrasound Targeted Microbubble Destruction

Liao

Chen

Wáng

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

Angiogenesis plays a vital part in the pathogenesis and treatment of cardiovascular disease and has become one of the hotspots that are being discussed in the past decades. At present, the promising angiogenesis therapies are gene therapy and stem cell therapy. Besides, a series of studies have shown that the ultrasound targeted microbubble destruction (UTMD) was a novel gene delivery system, due to its advantages of noninvasiveness, low immunogenicity and toxicity, repeatability and temporal and spatial target specificity; UTMD has also been used for angiogenesis therapy of cardiovascular disease. In this review, we mainly discuss the combination of UTMD and gene therapy or stem cell therapy which is applied in angiogenesis therapy in recent researches, and outline the future challenges and good prospects of these approaches.

show abstract

Transfection of cells in suspension by ultrasound cavitation

Cited by 42 publications

References 42 publications

Development of a custom biological scaffold for investigating ultrasound-mediated intracellular delivery

Development of a custom biological scaffold for investigating ultrasound-mediated intracellular delivery

Determination of the enzymatic activity of cytosolic 5′-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models

New Progress in Angiogenesis Therapy of Cardiovascular Disease by Ultrasound Targeted Microbubble Destruction

Contact Info

Product

Resources

About