Transfection of Human Prostate Cancer CA-HPV-10 Cells with Cytosolic Sulfotransferase SULT1E1 Affects Estrogen Signaling and Gene Transcription

Abstract

ABSTRACT:Human cytosolic sulfotransferase SULT1E1 catalyzes the sulfation of estrogens and estrogenic drugs in human reproductive tissues. Logically, this estrogen-preferring sulfotransferase isoform could play a regulatory role in estrogen signaling activities in human reproductive cells, including the prostate cells. This hypothesis was tested using DNA microarray and real-time reverse transcription-polymerase chain reaction methods in the present work. Potential changes in the transcriptional expression of selected signal transduction-related genes in human prostate cancer CA-HPV-10 cell line after SULT1E1 transfection were examined by DNA microarray methods. Notable changes were observed in the mRNA expression levels of TFRC, a cell membrane transferrin receptor gene, and TMEPAI, a gene encoding a steroid-dependent mRNA product. Expression of TFRC was down-regulated, whereas expression of TMEPAI was up-regulated by SULT1E1 transfection in CA-HPV-10 cells. Data from the current studies also showed that the estrogen-induced estrogen response element activation in CA-HPV-10 cells was repressed after the cells were transfected with SULT1E1. These results indicate that SULT1E1 may function as a transcriptional mediator in human prostate cancer CA-HPV-10 cells.

Oxidative and electrophilic structural modification and catalytic regulation of human hydroxysteroid sulfotransferase 2a1 (hsult2a1)

“…Sulfated estrone, being a compound that carries a negative charge at physiological pH, is eligible to be transported across the cell membranes of breast carcinoma cells (Miki et al, 2006), where sulfatase can catalyze removal of the sulfuryl group and transform it back to estrone. Besides breast carcinoma, other carcinomas like endometrial (Utsunomiya et al, 2004), prostate (Kapoor and Sheng, 2008), as well as colon carcinoma (Sato et al, 2009) are also closely related with estrogen levels in situ as well as estrogen sulfotransferase activities. This implies that sulfotransferases play a significant role in the regulation of endocrine balance and related carcinogenesis.…”