Transfection of Protoplasts Prepared from Arabidopsis thaliana Leaves for Plant Virus Research

Hosoe, Naoi; Keima, Takuya; Fujimoto, Yuji; Hagiwara‐Komoda, Yuka; Hashimoto, Masayoshi; Maejima, Kensaku; Yamaji, Yutaka

doi:10.1007/978-1-4939-9635-3_8

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…The 35S:GFP effector was obtained from Dr. Nobutaka Mitsuda. The reporter construct was cotransformed together with the effectors into Col-0 protoplasts for the transcriptional activity assay described previously 94,95 . The ratios of LUC to REN activity were calculated to define the relative promoter activity.…”

Section: Protoplast Transient Expression Assaysmentioning

confidence: 99%

BPG4 regulates chloroplast development and homeostasis by suppressing GLK transcription factors and involving light and brassinosteroid signaling

Tachibana,

Abe,

Marugami

et al. 2024

Nat Commun

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Chloroplast development adapts to the environment for performing suitable photosynthesis. Brassinosteroids (BRs), plant steroid hormones, have crucial effects on not only plant growth but also chloroplast development. However, the detailed molecular mechanisms of BR signaling in chloroplast development remain unclear. Here, we identify a regulator of chloroplast development, BPG4, involved in light and BR signaling. BPG4 interacts with GOLDEN2-LIKE (GLK) transcription factors that promote the expression of photosynthesis-associated nuclear genes (PhANGs), and suppresses their activities, thereby causing a decrease in the amounts of chlorophylls and the size of light-harvesting complexes. BPG4 expression is induced by BR deficiency and light, and is regulated by the circadian rhythm. BPG4 deficiency causes increased reactive oxygen species (ROS) generation and damage to photosynthetic activity under excessive high-light conditions. Our findings suggest that BPG4 acts as a chloroplast homeostasis factor by fine-tuning the expression of PhANGs, optimizing chloroplast development, and avoiding ROS generation.

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Section: Protoplast Transient Expression Assaysmentioning

confidence: 99%