Transfection of striped jack nervous necrosis virus (SJNNV) RNA into fish cells

Iwamoto,; Nakai,; Mori, Taketoshi; Arimoto,; Mise,; Furusawa,

doi:10.1046/j.1365-2761.2001.00283.x

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…Not all fish cells are susceptible to betanodaviruses infection [4,11,17,26], and the determinants of the host range of betanodavirus infectivity have not yet been clearly identified. It has been demonstrated that the RNA2-encoded coat protein controls host specificity.…”

Section: Discussionmentioning

confidence: 99%

“…RNA was extracted from purified virus preparations using Trizol reagent. RNA transfection was performed as described previously [17] with minor modifications. The cells were grown on glass coverslips to 70-80% confluence and washed twice in fresh L-15 medium without FBS for the E-11 cells, and in DMEM for the human cells.…”

Section: Virion Rnas Preparation and Transient Transfectionmentioning

confidence: 99%

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Potential for the replication of the betanodavirus redspotted grouper nervous necrosis virus in human cell lines

et al. 2007

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The determination of the host ranges of viruses is important because of the possible emergence of infectious agents, which may result from the zoonotic transmission of animal viruses to humans. The family Nodaviridae, whose members are non-enveloped, positive-stranded bipartite RNA viruses, is comprised of the genera Alphanodavirus and Betanodavirus, whose members predominantly infect insects and fish, respectively. The alphanodaviruses can also infect suckling mice and suckling hamsters, resulting in paralysis and death. Pigs near the site of isolation of the Nodamura virus (NoV), an alphanodavirus, have been reported to have high levels of NoV neutralizing antibody, suggesting that they may be part of the natural host range of this virus. Betanodaviruses are the causative agents of viral nervous necrosis, which occurs in several species of fish. However, little is known regarding the mechanism of infection of these viruses. Whether betanodaviruses can infect hosts other than fish remains unclear. In this study, we examined the possibility that a betanodavirus, redspotted grouper nervous necrosis virus (RGNNV), can infect human cell lines and showed that this virus can attach to the cells but cannot penetrate them, although human cells can support the replication of the betanodavirus when viral RNAs are transfected. The betanodavirus in its present form cannot infect human cells.

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Section: Discussionmentioning

confidence: 99%

Section: Virion Rnas Preparation and Transient Transfectionmentioning

confidence: 99%

Potential for the replication of the betanodavirus redspotted grouper nervous necrosis virus in human cell lines

et al. 2007

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“…Previously, we demonstrated the pathogenicity to striped jack larvae of the progeny that was generated by transfection of SJNNV virion RNAs into E-11 cells (Iwamoto et al , 2001 ). rSJ obtained from in vitro transcripts in this study was pathogenic to striped jack larvae.…”

Section: Discussionmentioning

confidence: 99%

“…The infectivity of transcripts was examined by transfection with lipofectin reagent (Gibco BRL) into E-11 cells followed by 24 h of incubation at 25 °C, as described previously (Iwamoto et al , 2001 ). For infection analysis of progeny virus, media cultured in the same manner as above for 72 h were collected, inoculated onto fresh E-11 cells and incubated at 25 °C for 24 h. Cell infectivity of the transcripts and their progeny was examined by immunofluorescence staining using anti-SJNNV rabbit polyclonal antibody and FITC-conjugated swine immunoglobulin (Ig) to rabbit Ig (Dako), as described previously (Nguyen et al , 1996 ).…”

Section: Methodsmentioning

confidence: 99%

“…The studies of Frerichs et al (1996) and our own group (Iwamoto et al , 1999 , 2000 ) have revealed, however, that the striped snakehead cell line (SSN-1) (Frerichs et al , 1991 ) and the clonal cell line E-11 from the SSN-1 cells are useful for qualitative and quantitative analyses for all of the betanodaviruses, including SJNNV. Furthermore, it has been confirmed that the SJNNV genomic RNA is infectious when transfected into E-11 cells and that the progeny virus recovered from the cells is virulent to striped jack larvae (Iwamoto et al , 2001 ).…”

Section: Introductionmentioning

confidence: 96%

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Establishment of an infectious RNA transcription system for Striped jack nervous necrosis virus, the type species of the betanodaviruses

Iwamoto

Mise

Mori³

et al. 2001

Journal of General Virology

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A system has been established to produce infectious RNA transcripts for Striped jack nervous necrosis virus (SJNNV), the type species of the betanodaviruses, which infect fish. An enzymological analysis suggested that both RNA1 and RNA2 of SJNNV have a 5h cap. Both RNAs were largely resistant to 3h polyadenylation and ligation, suggesting the presence of an interfering 3h structure, while a small quantity of viral RNAs were polyadenylated in vitro. The complete 5h and 3h noncoding sequences of both segments were determined using the rapid amplification of cDNA ends method. Based on the terminal sequences obtained, RT-PCR was carried out and plasmid clones containing full-length cDNA copies of both RNAs, positioned downstream of a T7 promoter, were constructed. These plasmids were cleaved at a unique restriction site just downstream of the 3h terminus of each SJNNV sequence and were transcribed in vitro into RNA with a cap structure analogue. A mixture of the transcripts was transfected into the fish cell line E-11. Using indirect immunofluorescence staining with anti-SJNNV serum, fluorescence was observed specifically in these transfected cells ; this culture supernatant exhibited pathogenicity to striped jack larvae. Northern blot analysis of E-11 cells infected with the recombinant virus or SJNNV showed small RNA (ca. 0n4 kb) that was newly synthesized and corresponded to the 3h-terminal region of RNA1. Finally, the complete nucleotide sequences of these functional cDNAs (RNA1, 3107 nt ; RNA2, 1421 nt) were determined. This is the first report of betanodavirus cDNA clones from which infectious genomic RNAs can be transcribed.

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Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout

Bols

Pham

Dayeh

et al. 2017

In Vitro Cell.Dev.Biol.-Animal

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The literature on cell lines that have been developed from rainbow trout (RT) (Oncorhynchus mykiss) is reviewed to illustrate three new terms: invitromatics, invitrome, and invitroomics. Invitromatics is defined as the history, development, characterization, engineering, storage, and sharing of cell lines. RT invitromatics differs from invitromatics for humans and other mammals in several ways. Nearly all the RT cell lines have developed through spontaneous immortalization. No RT cell line undergoes senescence and can be described as being finite, whereas many human cell lines undergo senescence and are finite. RT cell lines are routinely grown at 18-22°C in free gas exchange with air in basal media developed for mammalian cells together with a supplement of fetal bovine serum. An invitrome is defined as the grouping of cell lines around a theme or category. The broad theme in this article is all the cell lines that have ever been created from O. mykiss, or in other words, the RT invitrome. The RT invitrome consists of approximately 55 cell lines. These cell lines can also be categorized on the basis of their storage and availability. A curated invitrome constitutes all the cell lines in a repository and for RT consists of 11 cell lines. These consist of epithelial cell lines, such as RTgill-W1, and fibroblast cell lines, such as RTG-2. RTG-2 can be purchased from a scientific company and constitutes the commercial RT invitrome. Cell lines that are exchanged between researchers are termed the informally shared invitrome and for RT consists of over 35 cell lines. Among these is the monocyte/macrophage cell line, RTS11. Cell lines whose existence is in doubt are termed the zombie invitrome, and for RT, approximately 12 cell lines are zombies. Invitroomics is the application of cell lines to a scientific problem or discipline. This is illustrated with the use of the RT invitrome in virology. Of the RT invitrome, RTG-2 was the most commonly used cell line to isolate viruses. Fifteen families of viruses were studied with RT invitrome. RT cell lines were best able to support replication of viruses from the Herpesviridae, Iridoviridae, Birnaviridae, Togaviridae, and Rhabdoviridae families.

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Transfection of striped jack nervous necrosis virus (SJNNV) RNA into fish cells

Cited by 7 publications

References 12 publications

Potential for the replication of the betanodavirus redspotted grouper nervous necrosis virus in human cell lines

Potential for the replication of the betanodavirus redspotted grouper nervous necrosis virus in human cell lines

Establishment of an infectious RNA transcription system for Striped jack nervous necrosis virus, the type species of the betanodaviruses

Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout

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