Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine

Corso, Thomas D.; Torres, Germán; Goulah, Christopher C.; Roy, Indrajit; Gambino, Angelo S.; Nayda, John; Buckley, T. F.; Stachowiak, Ewa K.; Bergey, Earl J.; Pudavar, Haridas E.; Dutta, Pratik; Bloom, David C.; Bowers, William J.; Stachowiak, Michal K.

doi:10.1016/j.molbrainres.2005.05.032

Cited by 26 publications

(19 citation statements)

References 21 publications

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“…2E). The efficiency of ORMOSIL-mediated gene transfer was comparable with the most effective ICP4(Ϫ) herpes simplex virus 1 vector previously used in our laboratory (27) and was higher than that seen with herpes simplex virus 1 amplicon vector (27).…”

Section: Resultsmentioning

confidence: 51%

“…The efficiency of ORMOSIL-mediated transfection equaled or exceeded that obtained in previous studies using an herpes simplex viral vector (27). Tissue damage was also observed, caused by associated helper virus during herpes simplex virus 1 vector-mediated intrabrain gene transfer (26,27).…”

Section: Discussionmentioning

confidence: 63%

“…Tissue damage was also observed, caused by associated helper virus during herpes simplex virus 1 vector-mediated intrabrain gene transfer (26,27). These pathological side effects illustrate problems that are associated with the in vivo gene transfers such as (i) carrier toxicity, (ii) injury due to immunological side effects, and (iii) conversion to a pathogenic form during the transfection process (37-39).…”

Section: Discussionmentioning

confidence: 99%

“…The pEGFP-N2 (DNA control), ORMOSIL (nanoparticle control), and ORMOSIL͞pEGFP-N2 were injected into adult mice of both sexes by using stereotaxic surgery with equivalent concentrations of control injected materials and ORMOSIL͞plasmid (26,27). Mice were anesthetized; an incision into the dorsal aspect of the head was made, exposing the cranium and the bregma, and a fine dental air-drill was used to drill a hole in the skull.…”

Section: Methodsmentioning

confidence: 99%

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Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

Bharali

Klejbor

Stachowiak

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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591

383

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Section: Resultsmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

Bharali

Klejbor

Stachowiak

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

591

383

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“…5 Herein, we have reported the preparation of cationic liposomes from the arginine head group with different counterions and a 3-hydrocarbon chain spacer containing synthetic lipids without using any helper lipids, 16 characterization of the lipoplexes in the presence of serum, and investigation of the lipoplex morphology. We have also demonstrated that arginine-based cationic lipids are the most efficient gene delivery vehicles to neuronal cells and HeLa cells when compared with lysine-based cationic assemblies and Lipofectamine™ 2000.…”

Section: Discussionmentioning

confidence: 99%

Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity

Sarker¹,

Aoshima²,

Hokama³

et al. 2013

IJN

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Background: Currently available gene delivery vehicles have many limitations such as low gene delivery efficiency and high cytotoxicity. To overcome these drawbacks, we designed and synthesized two cationic lipids comprised of n-tetradecyl alcohol as the hydrophobic moiety, 3-hydrocarbon chain as the spacer, and different counterions (eg, hydrogen chloride [HCl] salt or trifluoroacetic acid [TFA] salt) in the arginine head group. Methods: Cationic lipids were hydrated in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer to prepare cationic liposomes and characterized in terms of their size, zeta potential, phase transition temperature, and morphology. Lipoplexes were then prepared and characterized in terms of their size and zeta potential in the absence or presence of serum. The morphology of the lipoplexes was determined using transmission electron microscopy and atomic force microscopy. The gene delivery efficiency was evaluated in neuronal cells and HeLa cells and compared with that of lysine-based cationic assemblies and Lipofectamine™ 2000. The cytotoxicity level of the cationic lipids was investigated and compared with that of Lipofectamine™ 2000. Results: We synthesized arginine-based cationic lipids having different counterions (ie, HCl-salt or TFA-salt) that formed cationic liposomes of around 100 nm in size. In the absence of serum, lipoplexes prepared from the arginine-based cationic liposomes and plasmid (p) DNA formed large aggregates and attained a positive zeta potential. However, in the presence of serum, the lipoplexes were smaller in size and negative in zeta potential. The morphology of the lipoplexes was vesicular. Arginine-based cationic liposomes with HCl-salt showed the highest transfection efficiency in PC-12 cells. However, arginine-based cationic liposomes with TFA salt showed the highest transfection efficiency in HeLa cells, regardless of the presence of serum, with very low associated cytotoxicity. Conclusion:The gene delivery efficiency of amino acid-based cationic assemblies is influenced by the amino acids (ie, arginine or lysine) present as the hydrophilic head group and their associated counterions.

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Neurodegenerative Disease Diagnostics and Therapy ofTiO₂‐BasedNanoparticles

et al. 2020

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Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine

Cited by 26 publications

References 21 publications

Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity

Neurodegenerative Disease Diagnostics and Therapy ofTiO₂‐BasedNanoparticles

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Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine

Cited by 26 publications

References 21 publications

Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity

Neurodegenerative Disease Diagnostics and Therapy ofTiO2‐BasedNanoparticles

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Product

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Neurodegenerative Disease Diagnostics and Therapy ofTiO₂‐BasedNanoparticles