Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies

Han, Muri; Bae, Younsoo; Nishiyama, Nobuhiro; Miyata, Kanjiro; Oba, Makoto; Kataoka, Kazunori

doi:10.1016/j.jconrel.2007.05.012

Cited by 79 publications

(83 citation statements)

References 20 publications

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“…The effects of the chemical structures of polycations and PEG-polycations on transfection and cytotoxicity were elucidated using a multicellular tumor spheroid (McTS) model, 38) which is known to be a very useful three-dimensional in vitro model representing the morphological and functional features of in vivo avascular solid tumors. Various features of transfection with polyplex micelles, which have been difficult to observe in conventional monolayer cultures, were revealed by the McTS model in terms of cytotoxicity and the time-dependent behavior of transfected gene expression under three-dimensional microenvironments.…”

Section: Peg-pasp(det) Polyplex Micelles Pasp(det)mentioning

confidence: 99%

Study on Development of Polymeric Micellar Gene Carrier and Evaluation of Its Functionality

Oba

2013

Biological & Pharmaceutical Bulletin

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Polymeric micelles, which are formed by the self-assembly of block copolymers, can load various substances and have received attention as drug carriers. Negatively charged nucleic acids such as DNA or RNA are some of the drugs delivered by polyion complex-based polymeric micelles (polyplex micelles). Polyplex micelles are expected to be nonviral gene carriers instead of viral vectors, which have several problems including safety, the limited size of the loading gene, and productivity. In this review, recent studies by our group on smart polyplex micelles delivering genes will be introduced.

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Section: Peg-pasp(det) Polyplex Micelles Pasp(det)mentioning

confidence: 99%

Study on Development of Polymeric Micellar Gene Carrier and Evaluation of Its Functionality

Oba

2013

Biological & Pharmaceutical Bulletin

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“…Over the past decades, self-assembled copolymer micelles consisting of amphiphilic block copolymer in aqueous medium are receiving considerable attention as gene and drug nanocarriers because of their particular characteristics [1][2][3][4]. Micelles always have a unique core-shell backbone composed of a hydrophilic shell and a hydrophobic core [5].…”

Section: Introductionmentioning

confidence: 99%

Docetaxel-Loaded Self-Assembly Stearic Acid-Modified Bletilla striata Polysaccharide Micelles and Their Anticancer Effect: Preparation, Characterization, Cellular Uptake and In Vitro Evaluation

et al. 2016

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Poorly soluble drugs have low bioavailability after oral administration, thereby hindering effective drug delivery. A novel drug-delivery system of docetaxel (DTX)-based stearic acid (SA)-modified Bletilla striata polysaccharides (BSPs) copolymers was successfully developed. Particle size, zeta potential, encapsulation efficiency (EE), and loading capacity (LC) were determined. The DTX release percentage in vitro was determined using high performance liquid chromatography (HPLC). The hemolysis and in vitro anticancer activity were studied. Cellular uptake and apoptotic rate were measured using flow cytometry assay. Particle size, zeta potential, EE and LC were 125.30 ± 1.89 nm, −26.92 ± 0.18 mV, 86.6% ± 0.17%, and 14.8% ± 0.13%, respectively. The anticancer activities of DTX-SA-BSPs copolymer micelles against HepG2, HeLa, SW480, and MCF-7 (83.7% ± 1.0%, 54.5% ± 4.2%, 48.5% ± 4.2%, and 59.8% ± 1.4%, respectively) were superior to that of docetaxel injection (39.2% ± 1.1%, 44.5% ± 5.3%, 38.5% ± 5.4%, and 49.8% ± 2.9%, respectively) at 0.5 µg/mL drug concentration. The DTX release percentage of DTX-SA-BSPs copolymer micelles and docetaxel injection were 66.93% ± 1.79% and 97.06% ± 1.56% in two days, respectively. Cellular uptake of DTX-FITC-SA-BSPs copolymer micelles in cells had a time-dependent relation. Apoptotic rate of DTX-SA-BSPs copolymer micelles and docetaxel injection were 73.48% and 69.64%, respectively. The SA-BSPs copolymer showed good hemocompatibility. Therefore, SA-BSPs copolymer can be used as a carrier for delivering hydrophobic drugs.

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“…Han et al reported that polyethylene glycol (PEG)-polyaspartamide with an ethylenediamine unit as a side chain (PEG-PAsp(DET)) exhibited single protonation in the side chain at a physiological pH of 7.4 while exhibiting double protonations at an endosomal pH of 5.5. 13) In NP-N, we expect that OH-N-Chol may show single protonation at neutral pH due to the strong electrostatic repulsion between two protonated amines in OH-N-Chol.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Plasmid DNA Transfer into Tumor Cells by Nanoparticle Composed of Cholesteryl Triamine and Diamine

Hattori

Nakamura

Ohno

et al. 2013

Biological & Pharmaceutical Bulletin

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Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies

Cited by 79 publications

References 20 publications

Study on Development of Polymeric Micellar Gene Carrier and Evaluation of Its Functionality

Study on Development of Polymeric Micellar Gene Carrier and Evaluation of Its Functionality

Docetaxel-Loaded Self-Assembly Stearic Acid-Modified Bletilla striata Polysaccharide Micelles and Their Anticancer Effect: Preparation, Characterization, Cellular Uptake and In Vitro Evaluation

Enhanced Plasmid DNA Transfer into Tumor Cells by Nanoparticle Composed of Cholesteryl Triamine and Diamine

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