Transfer Factor in Virus-Associated Malignancies: An Underestimated
                        Weapon in Prevention and Treatment of Cancer

Levine, Paul H.; Pizza, G; Ajmera, Kunal; Vinci, Caterina De; Viza, D.

doi:10.4137/atv.s7852

Cited by 5 publications

(1 citation statement)

References 73 publications

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“…While, higher survival rate in the TFt recipient mice confirmed the ability of the prepared TFt to protect the mice against challenge with this pathogen. Transfer factor is an immunoregulatory agent has been successfully used in the human and veterinary medicine as immunotherapy against the infections caused by various types of bacterial, viral, parasitic and fungal pathogens, as well as the diseases associated with defects in the cellular immune responses (Al-Graibawi et al, 2002;Levine et al, 2011;Viza et al, 2013 andZhou et al, 2015). The cellular immune responses play vital role in the host defences against pulmonary infection with P. aeruginosa (Pier and Markham, 1982;Buret et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Prepared Specific Pseudomonas aeruginosa Transfer Factor to Protect Mice against Experimental Challenge

Al-Graibawi¹,

Ati²

2016

Adv. Anim. Vet. Sci.

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| This study was conducted for the preparation and extraction of specific Pseudomonas aeruginosa transfer factor (TFt) from rats immunized with this pathogen and to evaluate its efficacy to protect the recipient mice against challenge with virulent P. aeruginosa. Two groups of rats (each one of six rats) were used, the first group was immunized subcutaneously (S.C) twice in two -week intervals with the whole sonicated antigen, the second group (control) was inoculated S.C with phosphate buffer saline (PBS). The immunized and control rats were monitored daily for the appearances of any clinical signs along one week post each immunization, and checked for the delayed hypersensitivity (DTH). Only the immunized rats revealed positive skin test reaction. The TFt were extracted from the spleen cells of the immunized rats, tested for sterility and safety. To evaluate the efficiency of the prepared TFt, three groups of mice (each of ten) were used. The first group was injected intraperiloneally (I/P) with 0.5 mL of TFt equivalent to 5x10 8 cells/mL, the second group (positive control) was immunized S.C with 0.5 mL of sonicated P. aeruginosa, while the third group injected S.C with 0.5 mL PBS (negative control). Later on, all the mice were tested by DTH -skin test. The immunized and TFt recipient mice showed a positive skin reaction, while the control group did not reveal any skin reaction. One week later, all the mice were challenged by I.P inoculation of 0.5 mL containing 5x10 7 CFU of P. aeruginosa, the survival rates were 90%, 80% and 10% in vaccinated, TFt recipient and control mice respectively. The results of the current study demonstrated the ability of the TFt to transport the specific DTH-skin reaction to the recipient non sensitized mice in addition, to its efficacy as immunotherapy to protect them against experimental challenge with P. aeruginosa.

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Section: Discussionmentioning

confidence: 99%

The Efficacy of Prepared Specific Pseudomonas aeruginosa Transfer Factor to Protect Mice against Experimental Challenge

Al-Graibawi¹,

Ati²

2016

Adv. Anim. Vet. Sci.

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Quality consistency evaluation of commercial transfer factor injections by chromatographic fingerprint combined with multivariate statistical analysis

Huang

Qian

Dong

et al. 2020

J of Separation Science

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The current quality control methods relying mainly on chromogenic reaction can hardly ensure the quality and safety of the biochemical drug with complex chemical composition. Therefore, a chromatographic fingerprint method was developed for the quality evaluation of a multicomponent biochemical drug, transfer factor injection. High-performance liquid chromatography fingerprint was measured by using a C 18 column (250 × 4.6 mm, 5 µm) with a mobile phase composed of 0.1% trifluoroacetic acid-water and 0.085% trifluoroacetic acid-acetonitrile under gradient elution. The developed method was validated and was subsequently applied to 57 batches of commercial products which were sampled by National Drug Assessment Program.High-resolution mass spectrometry analysis was performed on characteristic peaks of fingerprints, and a series of amino acids, nucleosides, and deoxynucleosides were identified. In the fingerprint assessments, principal component analysis and Hotelling T 2 analysis yielded the best results. The results generally indicated that there was a significant difference among products of batch-to-batch or from different manufacturers. Abnormal samples and its discriminatory components were also explored. In summary, the established fingerprinting method with multivariate statistical analysis could offer an efficient, reliable, and practical approach for quality consistency evaluation of transfer factor injection, providing a reference for the quality control of other multicomponent biochemical drugs. K E Y W O R D Shigh-performance liquid chromatography fingerprinting, multivariate statistical analysis, multicomponent biochemical drugs, transfer factor injection, quality consistency evaluation 2042

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Transfer Factor: Myths and Facts

Macías

Guaní-Guerra

2020

Archives of Medical Research

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Transfer Factor in Virus-Associated Malignancies: An Underestimated Weapon in Prevention and Treatment of Cancer

Cited by 5 publications

References 73 publications

The Efficacy of Prepared Specific Pseudomonas aeruginosa Transfer Factor to Protect Mice against Experimental Challenge

The Efficacy of Prepared Specific Pseudomonas aeruginosa Transfer Factor to Protect Mice against Experimental Challenge

Quality consistency evaluation of commercial transfer factor injections by chromatographic fingerprint combined with multivariate statistical analysis

Transfer Factor: Myths and Facts

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