Transfer functions of the conjugative integrating element pSAM2 from Streptomyces ambofaciens: characterization of a kil-kor system associated with transfer

Hagège, J; Pernodet, Jean‐Luc; Sezonov, Guennadi; Gerbaud, Claude; Friedmann, Annick; Guérineau, Michel

doi:10.1128/jb.175.17.5529-5538.1993

Cited by 56 publications

(73 citation statements)

References 55 publications

(83 reference statements)

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“…The kil-kor systems of the AICEs, like the kil-kor systems found on conjugative and pock forming Streptomyces plasmids, are associated with conjugation, in which Kor transcriptionally represses transfer genes responsible for the Kil-phenotype. The proposed function is to retard growth of recipient cells, resulting in pock formation, until the copy number of the AICE is sufficiently high (Hagege et al, 1993).…”

Section: Regulationmentioning

confidence: 99%

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

Poele

Samborskyy

Oliynyk

et al. 2008

Plasmid

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Section: Regulationmentioning

confidence: 99%

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

Poele

Samborskyy

Oliynyk

et al. 2008

Plasmid

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“…teins both have about the same degree of resemblance to the Streptomyces korSA protein (Hagege et al 1993) as they do to each other. Proteins encoded by ORFs 1,14,36,41,43,49, GL-3, GL-8, GL-21, and GL-31 do not resemble any- Fig.…”

Section: Gene Identificationmentioning

confidence: 75%

“…The korSA protein down regulates the intermycelial transfer of the plasmid (Hagege et al 1993). The GL-32 and ORF 2 proteins of pFQ12 and pFQ11 may well have a similar function, suggesting that both plasmids may be capable of conjugative transfer.…”

Section: Discussionmentioning

confidence: 99%

Analysis of pFQ12, a 22.4-kb <i>Frankia</i> plasmid

John¹,

Rice²,

Johnson³

2001

Rev. can. microbiol.

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Frankia are gram-positive, filamentous bacteria capable of fixing atmospheric dinitrogen in symbiosis with a wide variety of woody plants and shrubs. Some isolates of Frankia harbor plasmids of 8.5 (pFQ11) and 22.4 kb (pFQ12) that have no known function but are transmitted through many generations in culture. We have sequenced the 22 437-bp pFQ12 plasmid that is present in isolates CpI1 and ArI3. This sequence, with 76% G+C, is almost totally unrelated to that of pFQ11 found in the same cells. However, four regions of identity, 40-90 bp each, are dispersed around the plasmids. The 22.4-kb plasmid has >50 open reading frames (ORFs) that encode putative proteins of more than 100 amino acids, with the largest being 2226 amino acids. Twenty of these ORFs are likely to encode proteins based on their codon bias as determined by two different algorithms. Transcripts from nine of these regions have been identified by reverse transcriptase-polymerase chain reaction (RT-PCR) or filter hybridization. The two Frankia plasmids each encode a protein similar to the korSA protein that regulates transmission of pSAM2 in Streptomyces. The origin of replication (ORI) region of pFQ12 was localized by intrastrand AT and GC equivalence switch. It includes a 40-bp, intergenic, A+T-rich region that has a strong identity in pFQ11.Key words: ORI analysis, RT-PCR, Glimmer, DNA sequence.Résumé : Frankia est une bactérie filamenteuse gram-positive capable de fixer le diazote atmosphérique lorsqu'en symbiose avec une grande variété de plantes ligneuses et d'arbustes. Certains isolats de Frankia contiennent des plasmides de 8,5 (pFQ11) et 22,4 kb (pFQ12) qui n'ont aucune fonction connue mais qui sont transmises à travers plusieurs gé-nérations en culture. Nous avons séquencé le plasmide pFQ12 de 22 437 pb qui est présent dans les isolats CpI1 et ArI3. Cette séquence, constituée à 76 % de G+C, est presque totalement non apparentée à celle de pFQ11 retrouvé dans les mêmes cellules. Cependant, quatre régions d'identité, de 40-90 pb chaquene, sont dispersées autour des plasmides. Le plasmide de 22,4 kb contient >50 cadres de lecture ouverts « CLO » dant des protéines putatives de plus de 100 acides aminés, la plus grosse étant composée de 2226 acides aminés. Vingt d'entre ceux-ci sont susceptibles de coder des protéines, en se basant sur leur préférence en codons tel que déterminé par deux algorithmes différents. Les transcrits de neuf de ces régions ont été identifiés par RT-PCR ou par hybridation sur filtre. Les deux plasmides de Frankia codent chacune une protéine semblable à la protéine korSA qui contrôle la transmission de pMSA2 dans Streptomyces. La région ORI (« origin of replication ») de pFQ12 a été localisée par la transition de l'équivalence en AT et GC dans l'ADN. Elle renferme une région intergénique AT-riche de 40 pb qui démontre une forte analogie avec pFQ11.Mots clés : analyse ORI, RT-PCR, Glimmer, séquence d'ADN.[Traduit par la Rédaction] 617 John et al.

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“…Numerous Streptomyces plasmids contain plasmid transfer proteins with similarity to the SpoIII/FtsK family of septal DNA translocator proteins shown to be involved in plasmid transfer (Brolle et al, 1993;Hagege et al, 1993;Kieser et al, 1982;Maas et al, 1998;Pettis & Cohen, 1994;Vrijbloed et al, 1995b). pSLV45 contains two proteins, TraSLVA and TraSLVB, that showed significant similarity to SpoIII/FtsK proteins.…”

Section: Sequence Analysis and Deduced Functions Of Pslv45 Proteinsmentioning

confidence: 99%

“…SLV.4 and SLV.9 encode proteins of 110 and 113 aa, respectively, and have similarity to CAC36629.1, a 201 aa protein from S. coelicolor. They are also duplicated proteins that contain a high degree of suggests that it may regulate the transfer process of pSLV45, which would be similar to other GntR regulatory proteins in actinomycete plasmids (Grohmann et al, 2003).Numerous Streptomyces plasmids contain plasmid transfer proteins with similarity to the SpoIII/FtsK family of septal DNA translocator proteins shown to be involved in plasmid transfer (Brolle et al, 1993;Hagege et al, 1993;Kieser et al, 1982;Maas et al, 1998;Pettis & Cohen, 1994;Vrijbloed et al, 1995b). pSLV45 contains two proteins, TraSLVA and TraSLVB, that showed significant similarity to SpoIII/FtsK proteins.…”

mentioning

confidence: 99%

Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45

Hosted¹,

Wang²,

Horan³

2004

Microbiology

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Streptomyces lavendulae IMRU 3455 contains two large linear plasmids designated pSLV45 (45 kb) and pSLV195 (195 kb). A cosmid, pSPRX604, containing 42 kb from pSLV45 was cloned and sequenced. pSLV45 was tagged with a hygromycin-resistance marker by homologous recombination to generate the derivatives pSLV45.680 and pSLV45.681. An apramycin-resistance marker was introduced into S. lavendulae IMRU 467 using the pSPR910 integration vector to yield the recipient strain SPW910. The self-transmissible nature of pSLV45 was determined by transfer of pSLV45.680 and pSLV45.681 from the donor strains SPW680 and SPW681 into the recipient strain SPW910. Southern analysis indicated the presence of hygromycin-and pSLV45-hybridizing sequences within SPW910 exconjugants. PFGE analysis confirmed pSLV45.680 and pSLV45.681 were transferred intact and formed freely replicating linear plasmids. Sequence analysis of pSPRX604 revealed genes predicted to be involved in plasmid transfer, partitioning and regulation. The transfer of the linear plasmid pSLV45 from S. lavendulae IMRU 3455 into S. lavendulae IMRU 467 may allow the development of pSLV45 as an actinomycete-to-actinomycete conjugative shuttle vector. INTRODUCTIONLinear plasmids with 59 terminally attached proteins (TPs) and terminal inverted repeats (TIRs) have been found in bacteria, plants, filamentous fungi and yeasts, and often within actinomycetes (Griffiths, 1995;Hinnebusch & Tilly, 1993;Meinhardt et al., 1990;Rohe et al., 1992). Actinomycete linear plasmids pSLA1 (17 kb) and pSLA2 (17 kb) were first detected in the lankacidin-producer Streptomyces sp. 7434-AN4 and in Streptomyces rochei, respectively (Hayakawa et al., 1979;Hirochika & Sakaguchi, 1982;Hirochika et al., 1984). Both plasmids were shown to contain TIRs and TPs (Hirochika & Sakaguchi, 1982;Hirochika et al., 1984). With the advent of PFGE analysis, other actinomycete linear plasmids have been identified including Streptomyces lividans SLP2 (50 kb), Streptomyces clavuligerus pSCL1 (12 kb), Streptomyces avermitilis pSA1 and pSA2 (100 kb and 250 kb), Streptomyces rimosus pZG101 (387 kb), Streptomyces lasaliensis pKSL (520 kb) and Streptomyces coelicolor SCP1 (363 kb) Evans et al., 1994;Gravius et al., 1994;Keen et al., 1988;Kinashi & Shimaji, 1987;Kinashi & Shimaji-Murayama, 1991;Kinashi et al., 1994). All these plasmids were found to contain TIRs and TPs. In addition, both pSLA2 and SLP2 were shown to contain an internal origin of replication for bi-directional replication of DNA (Chang & Cohen, 1994;Chang et al., 1996;Huang et al., 2003).Unlike the chromosomes of most bacteria, which are circular in nature, the chromosomes of actinomycetes are linear (Lezhava et al., 1995;Lin et al., 1993). This was first demonstrated in S. lividans and has now been established for several other actinomycetes including S. coelicolor, Streptomyces griseus and Saccharopolyspora erythraea (formerly Streptomyces erythraeus) (Lezhava et al., 1995;Lin et al., 1993;Reeves et al., 1998). All actinomycete chromosomes characterized to da...

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Transfer functions of the conjugative integrating element pSAM2 from Streptomyces ambofaciens: characterization of a kil-kor system associated with transfer

Cited by 56 publications

References 55 publications

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

Analysis of pFQ12, a 22.4-kb <i>Frankia</i> plasmid

Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45

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