Transfer of a dominant gene for resistance to eyespot disease from a wild grass to hexaploid wheat

Doussinault, Gérard; Delibes, A.; Sánchez‐Monge, Rosa; Garcı́a-Olmedo, Francisco

doi:10.1038/303698a0

Cited by 97 publications

(65 citation statements)

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“…ventricosa/T. persicum//3*Marne) (Maia 1967;Doussinault et al 1983) and mapped to the long arm of chromosome 7D (Worland et al 1988). Since the 1950s, the French variety Cappelle-Desprez has been used as a source of the seedling-stage resistance provided by Pch2.…”

Section: Introductionmentioning

confidence: 99%

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

et al. 2007

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Hansen, Jennifer L.; Zemetra, Robert S.; Santra, Dipak K.; Campbell, Kimberly G.; and Riera-Lizarazu, Oscar, "Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1" (2008 Abstract Wheat is prone to strawbreaker foot rot (eyespot), a fungal disease caused by Oculimacula yallundae and O. acuformis. The most eVective source of genetic resistance is Pch1, a gene derived from Aegilops ventricosa. The endopeptidase isozyme marker allele Ep-D1b, linked to Pch1, has been shown to be more eVective for tracking resistance than DNA-based markers developed to date. Therefore, we sought to identify a candidate gene for Ep-D1 as a basis for a DNA-based marker. Comparative mapping suggested that the endopeptidase loci Ep-D1 (wheat), enp1 (maize), and Enp (rice) were orthologous. Since the product of the maize endopeptidase locus enp1 has been shown to exhibit biochemical properties similar to oligopeptidase B puriWed from E. coli, we reasoned that Ep-D1 may also encode an oligopeptidase B. Consistent with this hypothesis, a sequence-tagged-site (STS) marker, Xorw1, derived from an oligopeptidase B-encoding wheat expressed-sequence-tag (EST) showed complete linkage with Ep-D1 and Pch1 in a population of 254 recombinant inbred lines (RILs) derived from a cross between wheat cultivars Coda and Brundage. Two other STS markers, Xorw5 and Xorw6, and three microsatellite markers (Xwmc14, Xbarc97, and Xcfd175) were also completely linked to Pch1. On the other hand, Xwmc14, Xbarc97, and Xcfd175 showed recombination in the W7984 £ Opata85 RIL population suggesting that recombination near Pch1 is reduced in the Coda/Brundage population. In a panel of 44 wheat varieties with known eyespot reactions, Xorw1, Xorw5, and Xorw6 were 100% accurate in predicting the presence or absence of Pch1 whereas Xwmc14, Xbarc97, and Xcfd175 were less eVective. Thus, linkage mapping and a germplasm survey suggest that the STS markers identiWed here should be useful for indirect selection of Pch1.

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Section: Introductionmentioning

confidence: 99%

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

et al. 2007

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“…ventricosa was used to derive wheat lines with 42 chromosomes carrying genetic material from both the D v and the M v genomes (Delibes and García-Olmedo 1973;Delibes etal. 1977 a, b;Doussinault et al 1983;García-Olmedo et al 1984). In this report, we confirm the identification of the addition line carrying chromosome 4M V and characterize a transfer line carrying the genetic markers of that chromosome.…”

Section: Introductionmentioning

confidence: 99%

“…Triticum ventricosum) has been long recognized as a potential source of genes for resistance to fungi, insects and nematodes (Sprague 1936;Delibes et al 1977 a, b;Dosba and Doussinault 1977). Some of these genes, such as those conferring resistance to the eyespot disease (Pseudocercosporella herpotrichoides) and powdery mildew (Erysiphe graminis), have been already successfully transferred to the cultivated wheat Triticum aestivum (Delibes et al a, 1981(Delibes et al , 1987Doussinault et al 1983;García-Olmedo et al 1984), while work is in progress to further exploit Ae. ventricosa as a donor of useful agronomic traits.…”

Section: Introductionmentioning

confidence: 99%

“…In this context, a considerable effort has been devoted to develop addition lines carrying Ae. ventricosa chromosomes in a wheat background Delibes et al 1981;Dosba 1985) and to obtain substitution and transfer lines (Delibes and García-Olmedo 1973;Delibes et al 1977b;Doussinault et al 1983;García-Olmedo et al 1984), which are useful stocks in the manipulation of the resistance genes. Ae.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical and cytological characterization of wheat/Aegilops ventricosa addition and transfer lines carrying chromosome 4MV

Mena¹,

Orellana²,

López-Braña³

et al. 1989

Theoret. Appl. Genetics

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“…Wheat cultivar 'Holdfast' (susceptible) (Scott & Hollins, 1980), wheat line VPM 1 (resistant) (Doussinault et al, 1983) and rye cultivar 'Dominant' (resistant to T. yallundae) (Lange-de la Camp, 1966;Hollins et al,1985) were sown in plastic seed trays and inoculated with T. yallundae (47) or T. acuformis (48) using the straw cylinder inoculum method described by Hollins et al (1985). Six seedlings of each host were inoculated and five replicates made of each treatment.…”

Section: Infection Of Glasshouse-grown Wheat and Rye Seedlingsmentioning

confidence: 99%

Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay

et al. 1997

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Isolates of Tapesia yallundae and Tapesia acuformis were subjected to Random Amplified Polymorphic DNA (RAPD) assay. Amplification products common to isolates of either species were cloned and primers were generated from each sequence for use in conventional PCR. The primer pair derived from a T. yallundae specific RAPD marker amplified a product only from DNA of T. yallundae isolates and not from DNA of a range of other fungal species associated with the stem base disease complex of cereals. Similarly, the primer pair generated from a T. acuformis-specific RAPD marker amplifed a product only from DNA of T. acuformis isolates. Quantitative assays were developed for both species of Tapesia from these primer pairs, using competitive PCR. Competitive PCR was used to determine the level of colonization of seedlings by each species in glasshouse-and field-inoculated cereal hosts and results compared to those for conventional seedling disease assessment.

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Transfer of a dominant gene for resistance to eyespot disease from a wild grass to hexaploid wheat

Cited by 97 publications

References 3 publications

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

Biochemical and cytological characterization of wheat/Aegilops ventricosa addition and transfer lines carrying chromosome 4MV

Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay

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