Transfer of a starch phenotype from wild wheat to bread wheat by deletion of a locus controlling B-type starch granule content

Chia, Tansy; Adamski, Nikolai M; Saccomanno, Benedetta; Greenland, Andrew J.; Nash, A.L.; Uauy, Cristóbal; Trafford, Kay

doi:10.1093/jxb/erx349

Cited by 23 publications

(27 citation statements)

References 38 publications

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“…The mutant was found to have few, if any B-granules, like Ae. peregrina and a hexaploid bread wheat (Paragon) double-mutant with deletions of the BGC1-regions of chromosomes 4A and 4D (Chia et al, 2017). This result confirms that BGC1 is an ortholog of FLO6 and shows that FLO6 controls B-granule content in wheat and Aegilops.…”

Section: Discussionsupporting

confidence: 66%

“…We used the Aegilops genetic map to establish the syntenic interval in the reference hexaploid bread wheat genome of accession Chinese Spring. Our previous analysis of bread wheat deletion mutants showed that BGC1 is present in at least two genomes (A and D; Chia et al, 2017). We therefore simplified the synteny analysis by ignoring genes in the region of interest that are present in only one genome ( Fig.…”

Section: Floury Endosperm 6 Is a Strong Candidate For Bgc1mentioning

confidence: 99%

“…As a result, we hypothesised that other Triticeae species, such as bread wheat, have a gene controlling B-granule content, BGC1 in a syntenous position on the group 4 chromosomes. We tested this hypothesis in the bread wheat cultivar Paragon by selecting and combining together in one plant, large chromosomal deletions spanning the putative BGC1 regions of chromosomes 4A and 4D (Chia et al, 2017). The single-deletion mutants had normal starch, but the double-deletion mutant line was found to lack B-type starch granules.…”

Section: Introductionmentioning

confidence: 99%

“…The single-deletion mutants had normal starch, but the double-deletion mutant line was found to lack B-type starch granules. Thus, we successfully transferred the B-granule-less trait seen in Aegilops to bread wheat, and 4 showed that the BGC1 gene responsible for granule initiation must be one of the 240 genes that were common to both the 4A and 4D deletions (Chia et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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A carbohydrate-binding protein, FLOURY ENDOSPERM 6 influences the initiation of A- and B-type starch granules in wheat

Chia¹,

Chirico²,

King

et al. 2019

Preprint

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Previously, we identified a quantitative trait locus on the group 4 chromosomes of Aegilops and bread wheat that controls B-type starch-granule content. Here, we identify a candidate gene by fine-mapping in Aegilops and confirm its function using wheat TILLING mutants. This gene is orthologous to the FLOURY ENDOSPERM 6 (FLO6) gene of rice and barley and the PTST2 gene of Arabidopsis. In Triticeae endosperm, reduction in the gene dose of functional FLO6 alleles results in reduction, or loss, of B-granules. This is due to repression of granule initiation in lategrain development, but has no deleterious impact on the synthesis of A-granules.The complete absence of functional FLO6, however, results in reduced numbers of normal A-type and B-type granules and the production of highly-abnormal granules that vary in size and shape. This polymorphous starch seen in a wheat flo6 triple mutant is similar to that observed in the barley mutant Franubet. Analysis of Franubet (fractured Nubet) starch suggests that the mutant A-granules are not fractured but compound, due to stimulation of granule initiation in plastids during early-grain development. Thus, in different situations in Triticeae, FLO6 either stimulates or represses granule initiation.

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Section: Discussionsupporting

confidence: 66%

Section: Floury Endosperm 6 Is a Strong Candidate For Bgc1mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A carbohydrate-binding protein, FLOURY ENDOSPERM 6 influences the initiation of A- and B-type starch granules in wheat

Chia¹,

Chirico²,

King

et al. 2019

Preprint

Self Cite

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“…QTL mapping studies in the wild wheat Aegilops peregrina identified a gene locus, termed Bgc-1 that is located on the short arm of chromosome 4S, which appears to control the initiation or synthesis of B-granules [418]. Recent deletion studies in bread wheat detected orthologous Bgc-1 regions, which, when deleted, result in the loss of B-granules in the endosperm, but interestingly, no yield penalty [419]. The SS isoforms involved in granule initiation (SSIII and SSIV, see above) clearly play a role in determining granule size and number, but other proteins are likely to be involved.…”

Section: Starch Granule Growth Size and Morphologymentioning

confidence: 99%

Starch Biosynthesis in the Developing Endosperms of Grasses and Cereals

Tetlow

Emes

2017

Agronomy

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Abstract:The starch-rich endosperms of the Poaceae, which includes wild grasses and their domesticated descendents the cereals, have provided humankind and their livestock with the bulk of their daily calories since the dawn of civilization up to the present day. There are currently unprecedented pressures on global food supplies, largely resulting from population growth, loss of agricultural land that is linked to increased urbanization, and climate change. Since cereal yields essentially underpin world food and feed supply, it is critical that we understand the biological factors contributing to crop yields. In particular, it is important to understand the biochemical pathway that is involved in starch biosynthesis, since this pathway is the major yield determinant in the seeds of six out of the top seven crops grown worldwide. This review outlines the critical stages of growth and development of the endosperm tissue in the Poaceae, including discussion of carbon provision to the growing sink tissue. The main body of the review presents a current view of our understanding of storage starch biosynthesis, which occurs inside the amyloplasts of developing endosperms.

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A single base change at exon of Wx‐A1 caused gene inactivation and starch properties modified in a wheat EMS mutant line

et al. 2021

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BACKGROUND Wheat is an essential source of starch. The GBSS or waxy genes are responsible for synthesizing amylose in cereals. The present study identified a novel Wx‐A1 null mutant line from an ethyl methanesulfonate (EMS)‐mutagenized population of common wheat cv. SM126 using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and agarose gel analyses. RESULTS The alignment of the Wx‐A1 gene sequences from the mutant and parental SM126 lines showed only one single nucleotide polymorphism causing the appearance of a premature stop codon and Wx‐A1 inactivation. The lack of Wx‐A1 protein resulted in decreased amylose, total starch and resistant starch. The starch morphology assessment revealed that starch from mutant seeds was more wrinkled, increasing its susceptibility to digestion. Regarding the starch thermodynamic properties, the gelatinization temperature was remarkably reduced in the mutant compared to parental line SM126. The digestibility of native, gelatinized, and retrograded starches was analyzed for mutant M4‐627 and the parental SM126 line. In the M4‐627 line, rapidly digestible starch contents were increased, whereas resistant starch was decreased in the three types of starch. CONCLUSION Waxy protein is essential for starch synthesis. The thermodynamic characteristics were decreased in the Wx‐A1 mutant line. The digestibility properties of starch were also affected. Therefore, the partial waxy mutant M3‐627 might play a significant role in food improvement. Furthermore, it might also be used to produce high‐quality noodles. © 2021 Society of Chemical Industry.

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Transfer of a starch phenotype from wild wheat to bread wheat by deletion of a locus controlling B-type starch granule content

Abstract: We describe the production and characterization of bread wheat with grains that lack small B-type starch granules, have near-normal weight and starch content, and have novel grain and starch physicochemical properties.

Cited by 23 publications

References 38 publications

A carbohydrate-binding protein, FLOURY ENDOSPERM 6 influences the initiation of A- and B-type starch granules in wheat

A carbohydrate-binding protein, FLOURY ENDOSPERM 6 influences the initiation of A- and B-type starch granules in wheat

Starch Biosynthesis in the Developing Endosperms of Grasses and Cereals

A single base change at exon of Wx‐A1 caused gene inactivation and starch properties modified in a wheat EMS mutant line

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