Transfer of blood containing primordial germ cells between chicken eggs development of embryonic reproductive tract

Furuta, Hiroyuki; Sawada, Tomoko; Nishikawa, Kaoru; Yamamoto, Ichiro; Yoshida, Tatsuyuki; Tanaka, Minoru

doi:10.1007/s10616-007-9096-x

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…Furuta (2008) used histological techniques to study the morphology of homosexual and heterosexual PGCs transferred to embryos. Following heterosexual transfer, PGCs contributed to the development of gonads of sex opposite to that of the resident cells, but recipient embryos had abnormal gonads.…”

Section: Isolation Of Pgcs and Generation Of Pgc-derived Progenymentioning

confidence: 99%

Primordial germ cells (PGCs) as a tool for creating transgenic chickens

Chojnacka-Puchta¹,

Kasperczyk²,

Płucienniczak³

et al. 2012

Polish Journal of Veterinary Sciences

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The transgenic chicken has great potential as a bioreactor for the production of valuable pharmaceutical proteins, notably in the oviduct/egg. Whereas conventional transgenic approaches have significant limitations in this species, an alternative approach employing primordial germ cells (PGCs), the progenitor cells to ova and spermatozoa, has now been successfully applied to the insertion of exogenous genes into birds. Recent developments in manipulating avian embryos make it possible to produce germline chimeras derived from transferred PGCs. In this review we describe the migration pathway of chicken PGCs during early development. We then summarize different methods for the isolation of PGCs and the diversity of techniques used to introduce genes into these cells. Finally, we describe an in vitro assay for testing tissue-specific vectors designed to express heterologous proteins in transgenic chickens.

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Section: Isolation Of Pgcs and Generation Of Pgc-derived Progenymentioning

confidence: 99%

Primordial germ cells (PGCs) as a tool for creating transgenic chickens

Chojnacka-Puchta¹,

Kasperczyk²,

Płucienniczak³

et al. 2012

Polish Journal of Veterinary Sciences

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“…Numerous studies had reported the isolation and collection of PGCs from gonadal embryos (Nakamura et al, 2011;Nakajima et al, 2011;Nakajima et al, 2016). Moreover, germline chimeras had been produced in chicken by transferring PGCs or GGCs into the bloodstream at 2-day-old recipient embryos (Furuta et al, 2007(Furuta et al, , 2008 so that PGCs could be used as a genetic resource to produce germ line chimera (Furuta, 2012). Nakajima et al (2012) reported that gonadal PGCs collected from 7-day-old chick embryos and incubated in PBS [-] can be used to produce germline chimeras.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken

Sopiyana¹,

Setiadi

Fahrudin

et al. 2017

Med Pet

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Primordial germ cells (PGCs) are cells that will differentiate themselves into spermatogonia in the testis or oogonia in the ovary. Primordial germ cells arise from epiblast and circulate through the bloodstream and finally entering gonadal anlage. The aim of this study was to determine the number of gonadal PGCs of KUB chicken at different development stages. Sixty KUB chicken fertile eggs were divided into four groups (6, 7, 8, and 9 days incubation periods), and incubated at 38 o C with a humidity of 60%. Harvesting was synchronized to the embryonic development at 6-9 d. Gonads were collected using sharp tweezers, and were placed in Eppendorf tube 1.5 mL containing 500 µL PBS [-]. Gonadal PGCs were purified using PBS [-]. The results showed that the average number of gonadal PGCs at 6, 7, 8, and 9 d were 113.7; 143.5; 92.9; and 85.7 cells per embryo, respectively. Number of gonadal PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P<0.05), which reached a peak at day 7 of incubation, so that the isolation and collection of PGCs from the gonads were recommended at day 7 of incubation. This information is useful in production of germline chimera of other Indonesian local chickens. Keywords

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“…Another application to control the amount of water loss is cutting windows with several diameters on the blunt end of the eggshell. Windowing on the eggshell is usually used for studying chicken chimeras (Furuta et al, 1999(Furuta et al, , 2001(Furuta et al, , 2007(Furuta et al, , 2008. It has been reported that windowing application on eggshell elevated water evaporation (Bednarczyk et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Development of a method to control the water evaporation of hatching eggs during incubation

et al. 2010

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Three experiments were conducted to develop methods to control the amount of water loss and to evaluate the metabolic effects of water condition in the White Leghorn breeder eggs during incubation. One hundred twenty, 54, and 90 Julia strain White Leghorn breeder eggs were incubated at 37.8 degrees C, 60% RH in experiments 1, 2, and 3. In experiment 1, eggs were drilled with various bore diameters of 0, 0.5, 1, 2, 3, 4, and 5 mm on the blunt end of the eggshell. In experiment 2, 4 x 4 mm(2) windows were cut into the eggs or the eggs were drilled with 5 holes of bore diameter 2 mm on the blunt end of eggshell. In experiment 3, eggs were drilled with 1, 3, 5, and 7 holes of diameter 2 mm on the blunt end of eggshell. Eggs were treated on d 3 of each experiment and the amount of water loss was recorded on d 19 of incubation. Embryo growth was evaluated in experiments 2 and 3. In addition, the livers of embryos were collected in the 0-, 1-, 3-, and 5-hole treatment groups after weighing eggs to determine 3-hydroxy acyl coenzyme A dehydrogenase activity. In experiment 1, although higher water loss was observed in all windowed eggs than in control, there were no differences in amount of water loss among all bore diameters. Accordingly, that was not successful to control amount of water loss. In experiment 2, higher water loss was observed in drilled eggs at the same levels in windowed eggs as in control. Drilling holes was a more useful treatment to control amount of water loss on incubated eggs than windowing. In experiment 3, amount of water loss increased linearly with increasing number of holes on the blunt end of eggshell. Hepatic 3-hydroxy acyl coenzyme A dehydrogenase activity increased with increasing the number of drilled holes.

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Transfer of blood containing primordial germ cells between chicken eggs development of embryonic reproductive tract

Cited by 6 publications

References 24 publications

Primordial germ cells (PGCs) as a tool for creating transgenic chickens

Primordial germ cells (PGCs) as a tool for creating transgenic chickens

Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken

Development of a method to control the water evaporation of hatching eggs during incubation

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