Transfer of conjugative plasmid pAMβ1 from Lactococcus lactis to mouse intestinal bacteria

Igimi, Shizunobu; Ryu, Chunsun; Park, S. H.; Sasaki, Yasuko; Sasaki, Takashi; Kumagai, Susumu

doi:10.1111/j.1472-765x.1996.tb00023.x

Cited by 29 publications

(22 citation statements)

References 16 publications

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“…There is evidence that gene transfer can occur within the intestinal tract of mice (51). The ycdB gene has an unknown function and is widespread in bacteria (some enteric bacteria have two copies) and some eukaryotes.…”

Section: Resultsmentioning

confidence: 99%

Complete Genome Sequence of the Prototype Lactic Acid Bacterium Lactococcus lactis subsp. cremoris MG1363

et al. 2007

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Lactococcus lactis is of great importance for the nutrition of hundreds of millions of people worldwide. This paper describes the genome sequence of Lactococcus lactis subsp. cremoris MG1363, the lactococcal strain most intensively studied throughout the world. The 2,529,478-bp genome contains 81 pseudogenes and encodes 2,436 proteins. Of the 530 unique proteins, 47 belong to the COG (clusters of orthologous groups) functional category "carbohydrate metabolism and transport," by far the largest category of novel proteins in comparison with L. lactis subsp. lactis IL1403. Nearly one-fifth of the 71 insertion elements are concentrated in a specific 56-kb region. This integration hot-spot region carries genes that are typically associated with lactococcal plasmids and a repeat sequence specifically found on plasmids and in the "lateral gene transfer hot spot" in the genome of Streptococcus thermophilus. Although the parent of L. lactis MG1363 was used to demonstrate lysogeny in Lactococcus, L. lactis MG1363 carries four remnant/satellite phages and two apparently complete prophages. The availability of the L. lactis MG1363 genome sequence will reinforce its status as the prototype among lactic acid bacteria through facilitation of further applied and fundamental research.Lactococcus lactis, a mesophilic fermentative bacterium producing lactic acid from sugar (hexose) fermentation, is an important industrial microorganism with extensive and diverse uses in food fermentation. Strains of L. lactis are used as defined mixtures or in undefined combinations with other lactic acid bacteria (LAB) in the production of fermented milk products. The organism has adapted to growth in milk under stringent human selection for better performance with respect to taste, flavor, and texture of dairy products, and this process continues today (57,98,99). In 1985, the "dairy streptococci" were reclassified into two L. lactis subspecies, Lactococcus lactis subsp. lactis (previously Streptococcus lactis) and Lactococcus lactis subsp. cremoris (previously Streptococcus cremoris), to distinguish them from the streptococci sensu stricto, which contain a number of notorious human pathogens (82, 83).The strain used in this study, L. lactis subsp. cremoris MG1363, is the international prototype for LAB genetics, and the knowledge gained from fundamental research on this strain has been exploited for a wide variety of biotechnological applications. The large and unstable complement of plasmid DNA of the parent strain, L. lactis NCDO712, was eliminated by employing UV treatment and protoplast-curing strategies in the early 1980s (41). The resultant plasmid-free strain, L. lactis MG1363, is robust and genetically amenable, which has facilitated the analysis of introduced lactococcal and heterologous DNA. Sophisticated systems have been developed for the expression of proteins and peptides in this strain, and it has been used as a cell factory for a wide variety of heterologous products (e.g., antimicrobials, including bacteriocins [50], bacteriop...

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Section: Resultsmentioning

confidence: 99%

Complete Genome Sequence of the Prototype Lactic Acid Bacterium Lactococcus lactis subsp. cremoris MG1363

et al. 2007

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“…Only a limited number of studies have investigated in vivo antibiotic resistance transfer of pAM␤1 plasmid from L. lactis donors to the human gastrointestinal microbiota (54) or to mouse intestinal bacteria (27). In vivo transfer of the Tn916 transposon was observed previously in the intestinal tract, but from E. faecalis donor strains (5).…”

Section: Discussionmentioning

confidence: 99%

Intra- and Interspecies Conjugal Transfer of Tn 916 -Like Elements from Lactococcus lactis In Vitro and In Vivo

Bogusławska

Zycka-Krzesinska

Wilcks

et al. 2009

Appl Environ Microbiol

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Tetracycline-resistant Lactococcus lactis strains originally isolated from Polish raw milk were analyzed for the ability to transfer their antibiotic resistance genes in vitro, using filter mating experiments, and in vivo, using germfree rats. Four of six analyzed L. lactis isolates were able to transfer tetracycline resistance determinants in vitro to L. lactis Bu2-60, at frequencies ranging from 10 ؊5 to 10 ؊7 transconjugants per recipient. Three of these four strains could also transfer resistance in vitro to Enterococcus faecalis JH2-2, whereas no transfer to Bacillus subtilis YBE01, Pseudomonas putida KT2442, Agrobacterium tumefaciens UBAPF2, or Escherichia coli JE2571 was observed. Rats were initially inoculated with the recipient E. faecalis strain JH2-2, and after a week, the L. lactis IBB477 and IBB487 donor strains were introduced. The first transconjugants were detected in fecal samples 3 days after introduction of the donors. A subtherapeutic concentration of tetracycline did not have any significant effect on the number of transconjugants, but transconjugants were observed earlier in animals dosed with this antibiotic. Molecular analysis of in vivo transconjugants containing the tet(M) gene showed that this gene was identical to tet(M) localized on the conjugative transposon Tn916. Primer-specific PCR confirmed that the Tn916 transposon was complete in all analyzed transconjugants and donors. This is the first study showing in vivo transfer of a Tn916-like antibiotic resistance transposon from L. lactis to E. faecalis. These data suggest that in certain cases food lactococci might be involved in the spread of antibiotic resistance genes to other lactic acid bacteria.

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“…In the diassociated model pAMβ1 has been transferred from L. reuteri to Enterococcus faecalis (Morelli et al, 1988). Interspecies conjugative transfer of tetracycline and erythromycin resistance plasmids from lactobacilli has been demonstrated previously in vitro (Gevers et al, 2003a;Ouoba et al, 2008;Feld et al, 2008 Transfer has been demonstrated in the GIT of rodents, both gnotobiotic (Feld et al, 2008;Jacobsen et al, 2007;Morelli et al, 1988) and those having an indigenous gut microbiota (Feld et al, 2008;Jacobsen et al, 2007;Schlundt et al, 1994;McConnell et al, 1991;Gruzza et al, 1994;Igimi et al, 1996). In addition, the in vivo transfer of vancomycin resistance has recently been shown between enterococci and probiotic lactobacilli in gnotobiotic mice (Mater et al, 2008).…”

Section: Evidence Of Potential Horizontal Gene Transfer Of Probiotic mentioning

confidence: 99%

Assessment of Antibiotic Resistance in Probiotic Lactobacilli

Fukao¹,

Yajima²

2012

Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium

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Transfer of conjugative plasmid pAMβ1 from Lactococcus lactis to mouse intestinal bacteria

Cited by 29 publications

References 16 publications

Complete Genome Sequence of the Prototype Lactic Acid Bacterium Lactococcus lactis subsp. cremoris MG1363

Complete Genome Sequence of the Prototype Lactic Acid Bacterium Lactococcus lactis subsp. cremoris MG1363

Intra- and Interspecies Conjugal Transfer of Tn 916 -Like Elements from Lactococcus lactis In Vitro and In Vivo

Assessment of Antibiotic Resistance in Probiotic Lactobacilli

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