Transfer of duck cell DNA sequences to the nucleus of 3T3 cells by Rous sarcoma virus.

Abstract

Peking duck cell nuclear DNA has no complementarity to RNA of Prague C Rous sarcoma virus (RSV). Upon infection of Peking duck cells by Prague C RSV, polynucleotide sequences complementary to Peking duck cell nuclear DNA can be detected in the high molecular weight RNA from progeny Peking duck cell passaged RSV (RSVpD) by RNA-DNA molecular hybridization. When 3T3 cells are subsequently infected by RSVpD, polynucleotide sequences complementary to Peking duck cell nuclear DNA can be detected in the 3T3 cell nuclear DNA by RNA-DNA molecular hybridization. The potential consequences of the transfer of the Peking duck cell nuclear DNA from the avian to the murine cells are discussed. After passage of Rous sarcoma virus (RSV) through certain host cells, the emergent virus has altered tissue affinities, antigenic properties, and host range (1-9). Altaner and Temin (7) postulated that these alterations were a result of recombination between viral and host genetic material. Consequently it was demonstrated that type C retroviruses are capable of associating host genetic material with the viral genome (7,(10)(11)(12)(13) (21,22). The medium was clarified by centrifugation at 3000 X g at 20 for 10 min. While the medium was being centrifugated, a second medium containing 20 mCi of [3H]uridine (44 Ci/mmol) was inoculated into the same roller bottle and the bottle returned to the incubator. The two media were alternated each hour during the remainder of the 10-hr harvesting. Media were then centrifuged in a Beckman zonal rotor at 35,000 X g at 10 for 60 min. The pellet containing virus was resuspended in 5% sucrose-TNE (10 mM Tris1HCl/150 mM NaCl/1 mM EDTA, pH 7.4), layered on 20% glycerol-TNE, and centrifugated in a Beckman SW 41 rotor at 40,000 X g at 1°for 60 min. Nucleic acid was extracted Abbreviations: RSV, Rous sarcoma virus; TNE, 10 mM Tris-HCI/150 mM NaCl/l mM EDTA, pH 7.4; NaDodSO4, sodium dodecyl sulfate. 4252 The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

Rous Sarcoma Virus

“…These findings were later extended by Baxt and Meinkoth [1978], who infected mouse cells with RSV passaged in ducks and detected duck-specific sequences in DNA from infected mouse cells. Restriction en zyme analysis of this interesting system should provide a final evaluation of a puta tive duck-specific sequence transduction.…”

Dexamethasone-stimulated expression of a proviral copy of mouse mammary tumor virus env mRNA

“…This phenomenon could occur quite often, but unless a particular biochemical property is selected for, such as env complementation (22,23), it is unlikely that these transcriptional events would be detected. It is possible that retroviruses could transduce cellular genes by a similar mechanism (4,26). Although viral 5' sequences would probably be necessary for this to occur, cellular RNAs having viral 5' sequences have been identified (16).…”