Transfer of Faenia rectivirgula Kurup and Agre 1983 to the Genus Saccharopolyspora Lacey and Goodfellow 1975, Elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to Species Level, and Emended Description of the Genus Saccharopolyspora

Korn-Wendisch, Felicitas; Kempf, Alexander; Grund, E.; Kroppenstedt, R. M.; Kutzner, H. J.

doi:10.1099/00207713-39-4-430

Cited by 93 publications

(26 citation statements)

References 20 publications

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“…Nitrates were not reduced by the environmental isolates, but ATCC strains showed a variable positive reaction (Table 2). These results are consistent with those described previously by Korn-Wendisch et al [7].…”

Section: IVsupporting

confidence: 83%

“…The 14 isolates clustered together and could be readily discerned from the other closely related actinomycetes used as controls. Qualitatively, the CFA composition of all isolates was like those described previously [7]. The dendrogram (Fig.…”

Section: IVmentioning

confidence: 82%

“…Plates were incubated at 52°C for 5 days. Fifty-six colonies with beige to orange-brown colour and an actinomycete-like morphology (filamentous) were isolated [7]. Identical isolates were grouped according to colony morphology.…”

Section: Environmental Sampling and Colony Selectionmentioning

confidence: 99%

“…Lysozyme resistance was determined according to Kurup et al [6], Korn-Wendish et al [7] and Gordon [12]. A 5-ml volume of 0.01 N filtersterilised (0.22 pm) lysozyme 100 mg in 100 ml of 0.01 N HCI solution was added to 100 ml of TSB plus glycerol 7%.…”

Section: Acid Production and Utilisation Of Carbohydrates As Sole Carmentioning

confidence: 99%

“…An identification scheme based on biochemical properties and microscopic and macroscopic observations has been proposed [6]. The re-assignment of the organism to the genus Saccharupolyspura from the genus Faenia and major characteristics associated with the species were described by Korn-Wensisch et al [7].…”

Section: Introductionmentioning

confidence: 99%

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Saccharopolyspora rectivirgula from Quebec dairy barns: application of simplified criteria for the identification of an agent responsible for farmer's lung disease

Duchaine¹,

Mériaux²,

Brochu

et al. 1999

Journal of Medical Microbiology

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Saccharopolyspora rectivirgula (Micropolyspora faeni) is one of the major agents responsible for farmer's lung disease, a form of hypersensitivity pneumonitis. It is frequently isolated from the air of contaminated barns. The identification of this actinomycete is difficult because most of its phenotypic characteristics are variable and classical tests are not easy to perform on actinomycetes. Fatty acid analysis is very useful for the identification of these strains, but is not available except in some research or reference laboratories. Morphological (microscopic and macroscopic observations), physiological and biochemical tests (growth properties; macromolecules degraded; citrate utilisation and acid production from carbohydrates; resistance to antibiotics, lysozyme and heat), cell wall and fatty acid analyses and IgG analyses with serum from patients with farmer's lung were performed on 12 environmental isolates presumed to be S. rectivirgula and two control strains of S. rectivirgula. From this, a simple and rapid scheme for the identification of this actinomycete is proposed: optimal growth temperature (55°C); colony appearance based on morphology (filamentous) and colour (beige to orange-brown); microscopic morphology (chains of spores on both aerial and substrate mycelium); growth on NaCl 10%; cell-wall analysis (type IV); and the verification of antibody response with serum from a patient with farmer's lung. This last criterion is important to confirm the immunogenicity of the strains identified as S. rectivirgula. This scheme provides an accurate and efficient way of identifying S. rectivirgula strains and evaluating exposure to this bacterium. The study shows the limited value and the lack of reproducibility of some classical biochemical tests.

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Section: IVsupporting

confidence: 83%

Section: IVmentioning

confidence: 82%

Section: Environmental Sampling and Colony Selectionmentioning

confidence: 99%

Section: Acid Production and Utilisation Of Carbohydrates As Sole Carmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Saccharopolyspora rectivirgula from Quebec dairy barns: application of simplified criteria for the identification of an agent responsible for farmer's lung disease

Duchaine¹,

Mériaux²,

Brochu

et al. 1999

Journal of Medical Microbiology

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Pseudonocardiaceae

Labeda¹,

Goodfellow²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Saccharopolyspora

Kim¹,

Goodfellow²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Sac.cha.ro.po.ly.spo'ra. N.L. n. Saccharum generic name of sugar cane; Gr. adj. polus many; Gr. n. spora a seed, and in biology a spore; N.L. fem. n. Saccharopolyspora the many spored (organism) from sugar cane. Actinobacteria / Actinobacteria / Pseudonocardiales / Pseudonocardiaceae / Saccharopolyspora Aerobic, Gram‐stain‐positive, non‐acid‐fast, nonmotile, catalase‐positive actinobacteria which form an extensively branched substrate mycelium that typically fragments into coccoid and/or rod‐shaped elements . In some species, the substrate hyphae remain intact or are partially transformed into chains of spores. Aerial hyphae, when present, generally differentiate into bead‐like chains of spores contained within a smooth sheath . Spores are borne in straight, flexuous, hooked, looped, or spiral chains. Spore surfaces can be hairy, smooth, spiny, rough, or warty. Substrate mycelia may be buff, brownish red, orange, or yellow and aerial mycelia are white to gray or pinkish white. Diverse compounds are used as sole carbon sources for energy and growth. Whole‐organism hydrolysates contain meso ‐diaminopimelic acid, arabinose, and galactose. Muramic acid moieties are N ‐acetylated. Cells contain tetrahydrogenated menaquinones with nine isoprene units as the predominant menaquinone, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylmethylethanolamine as major polar lipids, and fatty acid profiles rich in iso‐ and anteiso‐branched chain components, but lack mycolic acids . The phylogenetic position of Saccharopolyspora , as determined by 16S rRNA gene sequencing, is in the family Pseudonocardiaceae . DNA G + C content ( mol %): 66–77. Type species : Saccharopolyspora hirsuta Lacey and Goodfellow 1975, 02 99 78 AL .

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Transfer of Faenia rectivirgula Kurup and Agre 1983 to the Genus Saccharopolyspora Lacey and Goodfellow 1975, Elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to Species Level, and Emended Description of the Genus Saccharopolyspora

Cited by 93 publications

References 20 publications

Saccharopolyspora rectivirgula from Quebec dairy barns: application of simplified criteria for the identification of an agent responsible for farmer's lung disease

Saccharopolyspora rectivirgula from Quebec dairy barns: application of simplified criteria for the identification of an agent responsible for farmer's lung disease

Pseudonocardiaceae

Saccharopolyspora

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