Transfer of Human Leukocytes into Double-Knockout Pfp−/−Rag2−/− Mice Grafted with Human Skin: Increased Accumulation of Neutrophils in Human Dermal Microvessels

Ullrich, Sebastian; Schumacher, Udo; Ai, Maixing; Tiemann, Bastian; Gay, Steffen; Schechner, Jeffrey S.; Pober, Jordan S.; Gross, Wolfgang L.; Csernok, Elena

doi:10.1097/01.tp.0000144326.75485.b9

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…Skin Diseases (25 Models) [Pass et al, 1973;Krueger et al, 1975;Briggaman and Wheeler, 1976;Cubie, 1976;Haftek et al, 1981;Gilhar et al, 1989;Kim et al, 1992;Boehncke et al, 1994;Ueda et al, 1994;Nickoloff et al, 1995;Paslin et al, 1997;Takizawa et al, 1997;Sugai et al, 1998;Lazarova et al, 2000;Yamanaka et al, 2001;Zeigler et al, 2001;Zillikens et al, 2001;Boyman et al, 2004;Ullrich et al, 2004;Zone et al, 2004;Fairley et al, 2007;Mihara et al, 2012;Melican et al, 2013;Ghraieb et al, 2018;Zerboni et al, 2018] Models of human skin graft on mice were used to study dermatological conditions such as psoriasis, ichthyosis, epidermolysis bullosa, etc. They provide further insight into human skin disorders and offer an opportunity to test a variety of treatments.…”

Section: Resultsmentioning

confidence: 99%

Mouse Models for Human Skin Transplantation: A Systematic Review

Cristóbal

Asúnsolo

Sánchez

et al. 2021

Cells Tissues Organs

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Immunodeficient mouse models with human skin xenografts have been developed in the past decades to study different conditions of the skin. Features such as follow-up period and size of the graft are of different relevance depending on the purpose of an investigation. The aim of this study is to analyze the different mouse models grafted with human skin. A systematic review of the literature was performed in line with the PRISMA statement using MEDLINE/PubMed databases from January 1970 to June 2020. Articles describing human skin grafted onto mice were included. Animal models other than mice, skin substitutes, bioengineered skin, postmortem or fetal skin, and duplicated studies were excluded. The mouse strain, origin of human skin, graft dimensions, follow-up of the skin graft, and goals of the study were analyzed. Ninety-one models were included in the final review. Five different applications were found: physiology of the skin (25 models, mean human skin graft size 1.43 cm2 and follow-up 72.92 days), immunology and graft rejection (17 models, mean human skin graft size 1.34 cm2 and follow-up 86 days), carcinogenesis (9 models, mean human skin graft size 1.98 cm2 and follow-up 253 days), skin diseases (25 models, mean human skin graft size 1.55 cm2 and follow-up 86.48 days), and would healing/scars (15 models, mean human skin graft size 2.54 cm2 and follow-up 129 days). The follow-up period was longer in carcinogenesis models (253 ± 233.73 days), and the skin graft size was bigger in wound healing applications (2.54 ± 3.08 cm2). Depending on the research application, different models are suggested. Careful consideration regarding graft size, follow-up, immunosuppression, and costs should be analyzed and compared before choosing any of these mouse models. To our knowledge, this is the first systematic review of mouse models with human skin transplantation.

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Section: Resultsmentioning

confidence: 99%

Mouse Models for Human Skin Transplantation: A Systematic Review

Cristóbal

Asúnsolo

Sánchez

et al. 2021

Cells Tissues Organs

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“…Because lymphocytes rather than human polymorphonuclear leukocytes develop in these xenograft models, studies on granulocyte-dependent inflammatory diseases using grafted human cells have been hampered. However, if these difficulties will be overcome, the use of mice with transplanted human granulocytes [39,40], or animals expressing human Fc receptors [41], may prove an optimal system to study the autoantibody-induced tissue damage in vivo.…”

Section: Discussionmentioning

confidence: 99%

Why human pemphigoid autoantibodies do not trigger disease by the passive transfer into mice?

Sesărman¹,

Oswald²,

Chiriac³

et al. 2012

Immunology Letters

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“…SCID mice have impaired T- and B- lymphocyte development [ 26 , 27 ]. Rag1 or Rag2 knockout (Rag −/− ) which leads to T- and B- lymphocyte arrest in bone marrow may also be used [ 28 ].…”

Section: Basic Principles In Xenotransplantationmentioning

confidence: 99%

Blank Spots in the Map of Human Skin: The Challenge for Xenotransplantation

Cherkashina,

Morgun,

Rippa

et al. 2023

IJMS

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Most of the knowledge about human skin homeostasis, development, wound healing, and diseases has been accumulated from human skin biopsy analysis by transferring from animal models and using different culture systems. Human-to-mouse xenografting is one of the fundamental approaches that allows the skin to be studied in vivo and evaluate the ongoing physiological processes in real time. Humanized animals permit the actual techniques for tracing cell fate, clonal analysis, genetic modifications, and drug discovery that could never be employed in humans. This review recapitulates the novel facts about mouse skin self-renewing, regeneration, and pathology, raises issues regarding the gaps in our understanding of the same options in human skin, and postulates the challenges for human skin xenografting.

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Transfer of Human Leukocytes into Double-Knockout Pfp−/−Rag2−/− Mice Grafted with Human Skin: Increased Accumulation of Neutrophils in Human Dermal Microvessels

Cited by 3 publications

References 13 publications

Mouse Models for Human Skin Transplantation: A Systematic Review

Mouse Models for Human Skin Transplantation: A Systematic Review

Why human pemphigoid autoantibodies do not trigger disease by the passive transfer into mice?

Blank Spots in the Map of Human Skin: The Challenge for Xenotransplantation

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