Effective boundary conditions, correct to third order in a small parameter , are derived by homogenization theory for the motion of an incompressible fluid over a rough wall with periodic microindentations. The length scale of the indentations is l, and ¼ l=L (1, with L a characteristic length of the macroscopic problem. A multiple scale expansion of the variables allows to recover, at leading order, the usual Navier slip condition. At next order the slip velocity includes a term arising from the streamwise pressure gradient; furthermore, a transpiration velocity Oð 2 Þ appears at the fictitious wall where the effective boundary conditions are enforced. Additional terms appear at third order in both wall-tangent and wall-normal components of the velocity. The application of the effective conditions to a macroscopic problem is carried out for the Hiemenz stagnation point flow over a rough wall, highlighting the differences among the exact results and those obtained using conditions of different asymptotic orders.