Transfer of resistance against soil-borne wheat mosaic virus from Triticum monococcum to hexaploid wheat (T. aestivum)

Abstract

Resistance to soil-borne viruses as Soil-borne Wheat Mosaic Virus (SBWMV) has gained evident importance in wheat research and breeding. Very few varieties of bread and durum wheat are resistant to these viruses. Search for new sources of SBWMV is conducted worldwide and loci causing resistance have been described on the B and D genomes of tetraploid and hexaploid wheat. In the present study, a gene locus was identified on the A genome of Triticum monococcum and was successfully transferred to hexaploid wheat. In hexaploid wheat, it is expressed in a stable dominant manner. As this is the first SBWMV resistance gene located on the A genome, this locus is proposed as Sbm3.

Nutrients and soil structure influence furovirus infection of wheat

“…After 12 weeks in infected soil, the youngest leaves of each plant were harvested for the detection of SBCMV and SBWMV by Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) according Clark and Adams (1977), as explained in Schlegel et al (2022). 96-well plates were coated with IgG 1:100 (JKI-AS92 for SBCMV and JKI-AS69 for SBWMV, respectively) in coating buffer (0.05M sodium carbonate, pH 9,6; 0.02% NaN 3 ) for 4 h, washed four times with PBS pH 7,4 containing 0,05% Tween 20 using a Tecan ELISA washer (Tecan, Switzerland) and kept at -20°C until use.…”

“…Sbm1 and Sbm2 were described as translocation resistances (Hunger and Sherwood, 1984;Driskel et al, 2002;Bass et al, 2006), not preventing the infection of roots, but limiting viral loads in the upper part of the plants to low amounts. The resistance mechanism underlying Sbm3, a recently identified additional resistance gene located on the A genome is still unknown (Schlegel et al, 2022). As the resources to combat SBWMV/SBCMV infection are very limited, optimizing the deployment of resistant plants and understanding additional factors influencing the infection is important.…”