Transfer of Salmonella resistance and delayed hypersensitivity with murine-derived transfer factor

Smith, Randall A.; Esa, Ahmed H.; Stiff, Mary I.

doi:10.1128/iai.36.1.271-276.1982

Cited by 15 publications

(2 citation statements)

References 18 publications

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“…Morris et al (23) found that immunosuppression of B cells, but not T cells, in vaccinated mice permitted their avirulent vaccine strain to grow out in the host and to be lethal for the vaccinated animals. Smith et al (35) reported that spleen cells and splenic transfer factor prepared from mice given a live vaccine 2 weeks previously transferred delayed-type hypersensitivity to Salmonella and low levels of protection (25%) as measured at 30 days postchallenge, but did not specify the cell type responsible for the transfer of immunity. (We find that many hypersusceptible mice die between 30 and 60 days, so 30 days may not be a valid endpoint.)…”

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confidence: 99%

Immunity to Salmonella typhimurium infection in C3H/HeJ and C3H/HeNCrlBR mice: studies with an aromatic-dependent live S. typhimurium strain as a vaccine

Killar¹,

Eisenstein²

1985

Infect Immun

124

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Immunization with avirulent Salmonella typhimurium strain SL3235, a smooth, aroA derivative, was shown to induce high levels of resistance to challenge with virulent S. typhimurium in innately hypersusceptible C3H/HeJ mice and inherently resistant C3H/HeNCrlBR mice. Strain SL3235 is one of a class of avirulent aroA-derivatives made from various strains and species of SalmoneUla that are being considered as vaccine candidates for cattle and humans. This paper supports their efficacy and potential utility in this regard. In C3H/HeJ mice, immunity against over 1,000 50% lethal doses of virulent S. typhimurium was evident as early as 3 days after immunization and persisted for at least 7 months. Further, the vaccine was effective over a broad * Corresponding author. immunity against challenge with virulent S. typhimurium strains in both mouse strains. In addition, short-term crossprotection against Listeria monocytogenes is evident. The period of nonspecific resistance is accompanied by marked splenomegaly. Immune macrophage-rich fractions, but not immune T cells, were able to transfer protection into naive C3H/HeJ recipients. MATERIALS AND METHODS Mice. Female C3H/HeJ and C3H/HeNCrlBR mice, 8 weeks old, were purchased from Jackson Laboratories, Bar Harbor, Maine, and Charles River Laboratories, Wilmington, Mass., respectively. They were housed in plastic cages with Absorb-Dri for bedding. Purina Mouse Chow and fresh water were available ad libitum. Bacterial strains. S. typhimurium strain SL3235, aroA-(nonreverting), was obtained from Bruce Stocker, Depart

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mentioning

confidence: 99%

Immunity to Salmonella typhimurium infection in C3H/HeJ and C3H/HeNCrlBR mice: studies with an aromatic-dependent live S. typhimurium strain as a vaccine

Killar¹,

Eisenstein²

1985

Infect Immun

124

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“…We have recently demonstrated that Salmonella typhimurium-immune ICR Swiss and C3H/HeJ mice exhibit more than 0.22% Salmonella antigen-specific rosette-forming lymphocytes (RFL), which correlated directly with the ability of the recipients to survive a challenge with virulent Salmonella (3). TF prepared from the spleens of S. typhimurium-immune ICR Swiss mice can confer host protection and positive footpad swelling responses to nonimmune ICR Swiss and C3H/HeJ mice (23).…”

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confidence: 98%

In vitro effect of murine-derived transfer factor on Salmonella-specific rosette formation

Smith¹,

Esa²

1982

Infect Immun

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The splenic lymphocytes from Salmonella-immune ICR Swiss or C3H/HeJ mice formed >0.2% antigen-specific rosettes with sheep erythrocytes coated with a spent-medium protein antigen of Salmonella typhimurium. These rosetteforming lymphocytes were found to be sensitive to the effects of antithymocyte serum plus complement. Transfer factor prepared from the Salmonella-immune splenic lymphocytes of ICR Swiss mice was active in sensitizing nonimmune ICR Swiss or C3H/HeJ lymphocytes to form .0.2% rosettes with salmonella antigencoated sheep erythrocytes. These rosettes were also sensitive to antithymocyte serum and complement. Few rosettes were formed between the transfer factortreated lymphocytes and sheep erythrocytes coated with a Listeria protein antigen. A nonimmune dialysate preparation was inactive in sensitizing nonimmune lymphocytes, as indicated by a lack of rosette formation. Neither the immune transfer factor nor the nonimmune dialysate had any enhancing or abrogating effect upon rosette formation by splenic lymphocytes from Salmonella-immune mice. The enumeration of antigen-specific rosettes may be a useful means of assaying for transfer factor activity.Transfer factor (TF) prepared from lymphoid tissues of immune animals, including humans, has been shown to transfer delayed hypersensitivity and protective host immunity to a nonimmune animal or immunodeficient or immunocompromised human (9-11, 14, 15, 17, 18, 21, 24). The lymphocytes of TF recipients will frequently exhibit migration inhibition factor production and blastogenic responses when cultured with antigens to which a skin test showed the donor to be positive (18,21).We have recently demonstrated that Salmonella typhimurium-immune ICR Swiss and C3H/HeJ mice exhibit more than 0.22% Salmonella antigen-specific rosette-forming lymphocytes (RFL), which correlated directly with the ability of the recipients to survive a challenge with virulent Salmonella (3). TF prepared from the spleens of S. typhimurium-immune ICR Swiss mice can confer host protection and positive footpad swelling responses to nonimmune ICR Swiss and C3H/HeJ mice (23).The purpose of this study was to determine whether the Salmonella-specific TF can sensitize nonimmune splenic lymphocytes in vitro to form Salmonella antigen-specific RFL. MATERIALS AND METHODSMice. ICR Swiss female mice 5 to 6 weeks old were obtained from Harlan Industries, Inc., Indianapolis, Ind. C3H/HeJ female mice 8 to 10 weeks old were obtained from Jackson Laboratories, Bar Harbor, Maine. Ten animals were housed in each cage.Bacterial strains. S. typhimurium SR-11 (virulent) and S. typhimurium RIA (avirulent) were originally obtained from L. J. Berry, Bryn Mawr College, Bryn Mawr, Pennsylvania. The bacteria were maintained on brain heart infusion agar slants and transferred to brain heart infusion broth before being used to immunize or challenge mice. Listeria monocytogenes was maintained on sheep blood agar slants.Spent-medium antigen. A spent-medium antigen was prepared from the SR-11 strain cultured for 48 h in ...

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Paradoxes of Immunity and Immunosuppression in Salmonella Infection

Eisenstein

Dalal

Killar

et al. 1988

Host Defenses and Immunomodulation to Intracellular Pathogens

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Transfer of Salmonella resistance and delayed hypersensitivity with murine-derived transfer factor

Cited by 15 publications

References 18 publications

Immunity to Salmonella typhimurium infection in C3H/HeJ and C3H/HeNCrlBR mice: studies with an aromatic-dependent live S. typhimurium strain as a vaccine

Immunity to Salmonella typhimurium infection in C3H/HeJ and C3H/HeNCrlBR mice: studies with an aromatic-dependent live S. typhimurium strain as a vaccine

In vitro effect of murine-derived transfer factor on Salmonella-specific rosette formation

Paradoxes of Immunity and Immunosuppression in Salmonella Infection

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