Transfer of Wheat Streak Mosaic Virus Resistance from Agropyron intermedium into Wheat

Abstract

Wheat streak mosaic is a damaging disease of cultivated bread wheat, Triticum aestivum L. em Thell., in many parts of the world. No wheat cultivar has a high level of resistance to WSMV or its vector, the wheat curl mite, Eriophyes tulipae Keifer. Genes conferring resistance to wheat curl mite colonization have been transferred from Aegilops tauschii Coss., Crncl, and from Agropyron elongatum (Host) Beauv., Cmc2. A gene conditioning resistance to WSMV, Wsml, was transferred from Agropyron intermedium (Host) Beauv. into common wheat. To broaden the genetic base of wheat, new sources of WSMV resistance are continuously sought. This study reports a new source of WSMV resistance derived from A. intermedium and its transfer into hexaploid wheat. C-banding analysis was used to cytogenetically identify the A. intermedium chromosomes and chromosome arms in three WSMV resistant wheat-A. intermedium derivatives, designated as 6957,6961, and 6962. All lines were identified as disomic chromosome addition lines with an A. intermedium chromosome arm in common. Restriction fragment length polymorphism analysis was used to establish homoeology of the A. intermedium chromosome arm carrying the gene or gene complex conditioning resistance to WSMV. The arm belonged to the long arm of the wheat Group 4 chromosomes, and was designated as 4Ai#3L. The added A. intermedium chromosomes present in Lines 6957 and 6961 probably originated from centricfusion. The WSMV resistance of these lines is different from that conditioned by Wsml and may have significance in breeding WSMV resistant wheat cultivars. W HEAT STREAK MOSAIC is a serious disease of cultivated bread wheat, Triticum aestivum L. em Thell. (2n = 6x = 42), in the Great Plains of the USA and in other wheat-producing areas of the world. Yield losses caused by WSM in Kansas averaged 2.6% over the last 20 yr (Christian and Willis, 1993). In 1988, an epidemic of WSMV in Kansas resulted in an estimated loss of about 13%, corresponding to 1.1 billion kg (Sim et al.,

Reactions of Perennial Grain Accessions to Four Major Cereal Pathogens of the Great Plains

“…These entries, therefore, appear to be susceptible to WSM, but carry lower virus titer than wheat. PI 223231, PI 502359, and TA 12252 were resistant to WSM, which is consistent with previous work (9,14,15,28).…”

“…Resistance gene Wsm1 from T. intermedium confers resistance to WSM and to wheat curl mite colonization (14,15). Although this study did not test for mite resistance, the adaptive ability of wheat curl mites may limit the value of this type of resistance in a wheat monoculture.…”

“…), the perennial donors in perennial wheat breeding programs, are resistant to many of the diseases that infect annual wheat, including wheat streak mosaic (WSM), stripe rust, leaf rust, and barley yellow dwarf (BYD) (14,15,24,26). In greenhouse experiments, several perennial wheat germ plasm lines (Thinopyrum spp.…”

Agronomic and quality effects in winter wheat of a gene conditioning resistance to wheat streak mosaic virus

“…However, there is resistance to WSMV in some perennial wheat relatives. A resistance gene (Wsm1) was identified and transferred from intermediate wheat-grass [Thinopyrum intermedium (Host) Barkworth & DR Dewey] to wheat to limit infection by the virus and WCM colonization (Friebe et al, 1996). Transfer of this resistance traces to crosses between wheat and intermediate wheat-grass produced more than three decades ago (Wells et al, 1973(Wells et al, , 1982.…”

Resistance to Wheat streak mosaic virus identified in synthetic wheat lines

“…Buckworth and Dewey and Th. intermedium (Host) Buckworth and Dewey (Lay et al 1971, Stoddard et al 1987Friebe et al, 1996Friebe et al, , 2009). Wsm1 and Wsm3, which were were derived from different compensating Robertsonian translocations from Th.…”