Transferability of ISSR, SCoT and SSR Markers for Chrysanthemum × Morifolium Ramat and Genetic Relationships Among Commercial Russian Cultivars

Abstract

Characterization of genetic diversity in germplasm collections requires an efficient set of molecular markers. We assessed the efficiency of 36 new SCoT markers, 10 new ISSR markers, and 5 microsatellites for the characterization of genetic diversity in chrysanthemum core collection of 95 accessions (Russian and foreign cultivars). Seven new SCoT (SCoT12, 20, 21, 23, 29, 31, 34) and six new ISSR markers ((GA)8T, (CT)8G, (CTTCA)3, (GGAGA)3, (TC)8C, (CT)8TG) were efficient for the genetic diversity analysis in Chrysanthemum × morifolium collection. After STRUCTURE analysis, most Russian cultivars showed 20–50% of genetic admixtures of the foreign cultivars. Neighbor joining analysis based on the combination of SSR, ISSR, and SCoT data showed the best accordance with phenotype and origin compared to the separate analysis by each marker type. The position of the accessions within the phylogenetic tree corresponded with the origin and with some important traits, namely, plant height, stem and peduncle thickness, inflorescence type, composite flower and floret types, flower color, and disc color. In addition, several SCoT markers were suitable to separate the groups distinctly by the phenotypical traits such as plant height (SCoT29, SCoT34), thickness of the stem and peduncle (SCoT31, SCoT34), and leaf size and the floret type (SCoT31). These results provide new findings for the selection of markers associated with important traits in Chrysanthemum for trait-oriented breeding and germplasm characterization.

Population Analysis of Diospyros lotus in the Northwestern Caucasus Based on Leaf Morphology and Multilocus DNA Markers

“…SCoT markers are considered as "molecular marker genes" because they associated with start codons ATG and amplify the sequence between two different genes, and so could provide more information for breeding [2,34,36,48]. On the other hand, some studies on different plant species showed that ISSR markers were always more polymorphic than SCoTs [42,[49][50][51]. However, other studies reported the better polymorphism of SCoT markers, compared to ISSRs [52][53][54].…”

Start codon-targeted marker evaluation of genetic relationship and population structure in southern Nigerian fluted pumpkin (Telfairia occidentalis Hook F.) collection

“…(2017) and Samarina et al . (2021). This discrepancy could be attributed to several factors, including the heterogeneity of the plant material used, methodology deployed for polymorphic loci detection and the number of landraces employed (Adu et al ., 2019; An et al ., 2019, Merheb et al ., 2020), annealing sites present in the genome, as well as the primer sequence (Moniruzzaman et al ., 2019).…”

Morphological, Biochemical, and Molecular Diversity Assessment of Egyptian Bottle Gourd Cultivars

“…Te evaluation of genetic variation in bottle gourd using molecular markers allows more efcient usage of plant traits in improving cultivars suitable for high yield and stability [6]. Te SCoT markers (start codon targeted) technique is the type of molecular marker that depends on polymorphism in the short-conserved region fanking the start codon ATG [8][9][10].…”