Transferability of olive microsatellite loci across the genus Olea

Rallo, Pilar; Tenzer, Isabel; Gessler, C.; Baldoni, Luciana; Dorado, Gabriel; Martı́n, A.

doi:10.1007/s00122-003-1332-y

Cited by 54 publications

(36 citation statements)

References 35 publications

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“…Several molecular studies have discriminated various Greek olive cultivars (Fabbri et al, 1995;Besnard et al, 2000Besnard et al, , 2001aBelaj et al, 2001Belaj et al, , 2002Nikoloudakis et al, 2003;Rallo et al, 2003;Owen et al, 2005;Terzopoulos et al, 2005;Hagidimitriou et al, 2005;Montemurro et al, 2005;Díaz et al 2006b;Essadki et al, 2006;Omrani-Sabbaghi et al, 2007;Bracci et al, 2009). Greek genotypes seem to carry the same chloroplast DNA polymorphisms (Besnard et al, 2000) and the same mitotypes (ME1), except Amigdalolia, which carries the ME2 mitotype (Besnard et al, 2001b).…”

Section: Discussionmentioning

confidence: 99%

“…Omrani-Sabbaghi et al (2007) did not find any particular clustering between seven Greek genotypes using SSR markers. Fabbri et al (1995), using RAPD markers, Rallo et al (2003) using SSR markers, Montemurro et al (2005) using SSR andAFLP markers, andBracci et al (2009) using SSR markers, included only two Greek genotypes in their studies from which no relevant information could be deduced. Discrimination of Greek olive cultivars according to SSR marker analysis does not produce clusters similar to those resulting from ISSR (Terzopoulos et al, 2005) and RAPD (Nikoloudakis et al, 2003;Hagidimitriou et al, 2005) analyses.…”

Section: Discussionmentioning

confidence: 99%

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Molecular identification of Greek olive(Olea europaea) cultivars based on microsatellite loci

Roubos

Moustakas²,

Aravanopoulos

2010

Genet. Mol. Res.

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ABSTRACT.Olea europaea is one of the oldest species of domesticated trees. We used microsatellite markers for fingerprinting and for evaluation of genetic similarity and structure of 26 Greek olive cultivars, which cover most of the olive cultivation regions of Greece, including previously undescribed denominations from northern Greece. Eighty-one alleles were revealed with six SSR loci that were selected as most informative of 10 SSR primers that were initially investigated. The number of alleles per locus varied from 7 to 20 (mean, 13.5). Heterozygosity ranged from 0.240 at locus DCA-3 to 0.826 at locus UDO99-9, with a mean value of 0.600. Analysis of 104 trees representing 26 denominations (four trees per denomination) revealed 26 distinct SSR profiles, indicating 26 olive cultivars; no intracultivar variability was observed. Genetic and geographic distances were not significantly correlated, based on the Mantel test. These SSR loci allowed unequivocal identification of all the cultivars and will be useful for future breeding and olive germplasm management efforts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular identification of Greek olive(Olea europaea) cultivars based on microsatellite loci

Roubos

Moustakas²,

Aravanopoulos

2010

Genet. Mol. Res.

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“…This depends on the conservation of SSR flanking regions and primer binding sites in different taxa. Several studies have shown high conservation of these flanking regions in tree species, which explains the high tranferability of SSR among those species (Thomas and Scott, 1993;Kijas et al, 1995;Cipriani et al, 1999;Sefc et al, 1999;Billotte et al, 2001;Hodgetts et al, 2001;Yamamoto et al, 2001;Dirlewanger et al, 2002;Wunsch and Hormaza, 2002;Rosseto et al, 2002;Shepherd et al, 2002;Decroocq et al, 2003;Rallo et al, 2003). However, transferability in the Gramineae has generally been poor where this has been tested (Brown et al, 1996).…”

Section: Ssr Transferabilitymentioning

confidence: 99%

“…A high conservation of microsatellite loci has been observed between tree taxa. This conservation has propitiated high transferability indexes between Citrus (Kijas et al, 1995), Prunus (Dirlewanger et al, 2002;Wunsch and Hormaza, 2002;Decroocq et al, 2003), Vitis (Sefc et al, 1999), Elaeis (Billotte et al, 2001), Picea (Hodgetts et al, 2001), Pinus (Shepherd et al, 2002), Malus and Pyrus (Yamamoto et al, 2001), and Olea (Rallo et al, 2003). Already in Gramineae, in the few accomplished studies, the transferability of microsatellite markers has been low (Brown et al, 1996;Bryan et al, 1997;Röder et al, 1998;Stephenson et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Transferability and utility of white oat (Avena sativa) microsatellite markers for genetic studies in black oat (Avena strigosa)

Silva

Milach²,

Tisian³

2011

Genet. Mol. Res.

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ABSTRACT. Preservation and use of wild oat species germplasm are essential for further improvement of cultivated oats. We analyzed the transferability and utility of cultivated (white) oat Avena sativa (AACCDD genome) microsatellite markers for genetic studies of black oat A. strigosa (A s A s genome) genotypes. The DNA of each black oat genotype was extracted from young leaves and amplified by PCR using 24 microsatellite primers developed from white oat. The PCR products were separated on 3% agarose gel. Eighteen microsatellite primer pairs amplified consistent products and 15 of these were polymorphic in A. strigosa, demonstrating a high degree of transferability. Microsatellite primer pairs AM3, AM4, AM21, AM23, AM30, and AM35 consistently amplified alleles only in A. sativa, which indicates that they are putative loci for either the C or D genomes of Avena. Using the data generated by the 15 polymorphic primer pairs, it was possible to separate 40 genotypes of the 44 that we studied. The four genotypes that could not be separated are probably replicates. We conclude that A. sativa microsatellites have a Genetic variability in black oat accessed by microsatellite high transferability index and are a valuable resource for genetic studies and characterization of A. strigosa genotypes.

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“…Rico et al, 1996) of different organisms, resulting less efficient among plants (Rosetto, 2001). However transferability of nuclear microsatellite loci across species was successful in eucalyptus (Myrtaceae, Zucchi et al, 2002), olives (Oleaceaes, Rallo et al, 2003), oaks (Fagaceae, Barreneche et al, 2004;Durand et al, 2010), carob tree (Fabaceae, Mottura et al, 2005), rubber tree (Euphorbiaceae, Feng et al, 2009) and loblolly pine (Pinaceae, Liewlaksaneeyanawin et al, 2004), among others.…”

Section: Introductionmentioning

confidence: 99%

Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana

et al. 2011

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Cross-species amplification of microsatellites is a common procedure to obtain suitable markers to be used in population genetic studies. Primers designed for one (source) species are used to amplify homologous loci in related (target) species. It is expected that phylogenetically close species will share a higher proportion of markers, and genetic distance could be a useful parameter to predict successful transferability between different taxonomic groups.We analyzed twenty-two primer pairs developed for Araucaria angustifolia (Bertol.) Kuntze in five target species of the Araucariaceae family. The results were summarized in vectors of presence and absence of bands and compared through the Jaccard similarity index. Using the sequences of eight published genes, genetic distances between pairs of species were estimated and related to transferability rate using Pearson correlations.Successful transfer rate ranged from 31.8 to 77.3%, being these among the highest reported for plants. The highest transfer rate was observed between the South American species. The transferability was confirmed sequencing seven fragments amplified in A. araucana (Molina) K. Koch, and using the best five to estimate genetic diversity parameters in a natural population of this Andean coniferous.Key word: cross species amplification; pairwise genetic distance; conservation of microsatellite loci; Pehuén. ResumenTransferibilidad cruzada de microsatélites a cinco especies de Araucariaceae: una herramienta útil para estudios de genética de poblaciones en Araucaria araucana La transferencia de cebadores que amplifican loci microsatélites desde otras especies es una práctica habitual para obtener marcadores adecuados para estudios de genética poblacional. Los cebadores diseñados en una especie (fuente) son utilizados para amplificar loci homólogos en especies relacionadas (blanco). Se espera que especies cercanas filogenéticamente compartan un mayor número de marcadores y la distancia genética entre especies podría ser un parámetro útil para predecir el éxito de la transferencia entre diferentes grupos taxonómicos.Se analizó la transferencia de veintidós primers, desarrollados para Araucaria angustifolia (Bertol.) Kuntze, a cinco especies blanco de la familia Araucariaceae. Los resultados se resumieron en vectores de presencia y ausencia de bandas y fueron comparados a través del índice de similitud de Jaccard. Se estimaron las distancias genéticas de a pares entre las especies analizadas utilizando las secuencias de ocho genes, y se las relacionó con el índice de transferencia utilizando correlaciones de Pearson.El éxito de la transferencia varió entre 31,8 y 77,3%, encontrándose estos valores entre los más altos reportados en plantas. El mayor índice de transferencia se verificó entre las especies sudamericanas. La transferencia fue confirmada secuenciando siete fragmentos amplificados en Araucaria araucana (Molina) K. Koch, y utilizando los cinco mejores para estimar parámetros de diversidad genética en una población natural de esta...

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Transferability of olive microsatellite loci across the genus Olea

Cited by 54 publications

References 35 publications

Molecular identification of Greek olive(Olea europaea) cultivars based on microsatellite loci

Molecular identification of Greek olive(Olea europaea) cultivars based on microsatellite loci

Transferability and utility of white oat (Avena sativa) microsatellite markers for genetic studies in black oat (Avena strigosa)

Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana

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