Transferencia eléctrica capacitiva (TEC). Técnica no invasiva de hipertermia profunda en el tratamiento de los gliomas cerebrales. Resultados preliminares

Ley, A.; Cladellas, J.M.; Colet, S.; Heras, P.; Florensa, R.; Prim, Joëlle; Roussos, J; Ariza, Aurelio; Calbet, José A. L.

doi:10.1016/s1130-1473(92)70894-x

Cited by 5 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pilot trials on patients with glioblastoma multiforme have reported that CRET treatment can reduce peritumoral edema and intratumoral vascularization, and induce extensive necrosis in the tumor core and significant tumor growth reduction, without affecting the surrounding healthy brain tissue (9). More recently, Kato et al (22) have shown that CRET treatment administered at thermal doses can significantly potentiate the anticancer effect of 6-O-palmitoyl-ascorbate on human tongue squamous carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells

et al. 2012

View full text Add to dashboard Cite

Recently, a number of electric and electrothermal therapies have been applied to the treatment of specific cancer types. However, the cellular and molecular mechanisms involved in the response to such therapies have not been well characterized yet. Capacitive-resistive electric transfer (CRET) therapy uses electric currents at frequencies within the 0.45–0.6 MHz range to induce hyperthermia in target tissues. Preliminary trials in cancer patients have shown consistent signs that CRET could slow down growth of tumor tissues in brain gliomas, without inducing detectable damage in the surrounding healthy tissue. Previous studies by our group have shown that subthermal treatment with 0.57-MHz electric currents can induce a cytostatic, not cytotoxic response in HepG2 human hepatocarcinoma cells; such effect being mediated by cell cycle alterations. In contrast, the study of the response of NB69 human neuroblastoma cells to the same electric treatment revealed consistent indications of cytotoxic effects. The present study extends the knowledge on the response of NB69 cells to the subthermal stimulus, comparing it to that of primary cultures of human peripheral blood mononuclear cells (PBMC) exposed to the same treatment. The results showed no sensitivity of PBMC to the 0.57 MHz subthermal currents and confirmed that the treatment exerts a cytotoxic action in NB69 cells. The data also revealed a previously undetected cytostatic response of the neuroblastoma cell line. CRET currents affected NB69 cell proliferation by significantly reducing the fraction of cells in the phase G2/M of the cell cycle at 12 h of exposure. These data provide new information on the mechanisms of response to CRET therapy, and are consistent with a cytotoxic and/or cytostatic action of the electric treatment, which would affect human cells of tumor origin but not normal cells with a low proliferation rate.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Preliminary assays of CRET therapy on cancer patients showed consistent signs of slowing down the growth of the tumor tissues, without adversely affecting the surrounding healthy tissues (9–11). Similar effects have been reported in response to other therapies based on capacitive hyperthermia (12–14).…”

Section: Introductionmentioning

confidence: 98%

Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells

et al. 2012

View full text Add to dashboard Cite

show abstract

“…This therapy has proved effective in restoring damaged tissues including muscle, tendon or vessels (8). Additionally, preliminary tests conducted in cancer patients suffering from glioblastoma multiforme have shown that CRet treatment induced necrosis and decreased tumor growth, which was interpreted as indicative of an oncostatic ability of the therapy (9,10). Despite these indications of potential oncological applications of CRet, the current knowledge about the cellular response to this and other radiofrequency electrothermal therapies is still very poor.…”

Section: Introductionmentioning

confidence: 99%

Cytostatic response of HepG2 to 0.57 MHz electric currents mediated by changes in cell cycle control proteins

Hernández-Bule

Cid²,

Trillo³

et al. 2010

Int J Oncol

View full text Add to dashboard Cite

Abstract. The capacitive-resistive electric transfer (CRet) therapy is a non-invasive technique that applies electrical currents of 0.4-0.6 MHz to the treatment of musculoskeletal injuries. Although this therapy has proved effective in clinical studies, its interaction mechanisms at the cellular level still are insufficiently investigated. Results from previous studies have shown that the application of CRet currents at subthermal doses causes alterations in cell cycle progression and decreased proliferation in hepatocarcinoma (HepG2) and neuroblastoma (NB69) human cell lines. The aim of the present study was to investigate the antiproliferative response of HepG2 to CRet currents. The results showed that 24-h intermittent treatment with 50 μA/mm 2 current density induced in HepG2 statistically significant changes in expression and activation of cell cycle control proteins p27Kip1 and cyclins D1, A and B1. The chronology of these changes is coherent with that of the alterations reported in the cell cycle of HepG2 when exposed to the same electric treatment. We propose that the antiproliferative effect exerted by the electric stimulus would be primarily mediated by changes in the expression and activation of proteins intervening in cell cycle regulation, which are among the targets of emerging chemical therapies. The capability to arrest the cell cycle through electrically-induced changes in cell cycle control proteins might open new possibilities in the field of oncology.

show abstract

“…CRet system can induce local hyperthermia induced in the target tissues through the mobile electrodes for transdermal application, and the treatment has been reported to be more effective and less edema formation than other thermal therapies [16]. Preliminary tests of CRet system on cancer patients also showed consistent signs of slowing down the growth of the tumor tissues without adverse effects in the surrounding healthy tissues [20,21]. Additonally, previous studies have shown that CRet treatment induces cytostatic effects in the human hepatocarcinoma HepG2 [16,17,19], cytotoxic effects in NB69 human neuroblastoma cells [18] and significantly potentiate anticancer effect of 6-O-palmitoyl-ascorbate on human tongue squamous carcinoma HSC-4 [22].…”

Section: Methodsmentioning

confidence: 99%

“…Capacitive-resistive electric transfer (CRet) system applies electric currents at frequencies within the 0.4-0.6 MHz range to treatment of inflammation and musculoskeletal injuries [16][17][18][19]. It has been demonstrated that CRet system, which can produce electric stimuli and thermal effects simultaneously in tissues, can induce synergistically cytostatic and/ or cytotoxic effects in tumor tissues [20,21]. Thus, CRet system has been studied as one of a novel application in the field of oncology.…”

mentioning

confidence: 99%

Synergic Carcinostatic Effects of Ascorbic Acid and Hyperthermia on Ehrlich Ascites Tumor Cell

Saitoh¹,

Yoshimoto²,

Kato³

et al. 2015

Exp. Onc.

View full text Add to dashboard Cite

Aim: In this study, we evaluated the carcinostatic effects of combined ascorbic acid (AsA) and a capacitive-resistive electric transfer (CRet) hyperthermic apparatus-induced hyperthermic treatment on Ehrlich ascites tumor (EAT) cells. Materials and Methods: EAT cells were exposed to various AsA (0–10 mM) concentrations for 1 h; they subsequently underwent CRet treatment for 15 min at 42 °C. Cell viability was assessed by the WST-8 assay 24 h after the combined treatment. Reactive oxygen species involvement was evaluated using catalase and tempol; caspase-3/7 activation was determined by their fluorescent substrates; cell proliferation were estimated by time-lapse observation. The effect on the cell cycle was analyzed by flow cytometry. Results: Combined AsA and CRet treatment synergistically suppressed cell viability compared with either treatment alone, and these synergistically carcinostatic effects were evident even at noncytotoxic concentrations of AsA alone (≤ 2 mM). The carcinostatic effects of combined AsA and CRet treatment were attenuated in a dose-dependent manner by catalase addition, but not by the superoxide anion radical scavenger tempol. Time-lapse observation revealed that combined AsA and CRet treatment activated caspase-3/7 at 10–24 h after treatment, accompanied by significant cell growth suppression. Cell cycle analysis revealed that the rate of sub-G1-phase (apoptotic) cells was drastically increased at 12 h and 24 h, and that the G2/M-phase cells gradually increased at 6–24 h after treatment. Conclusion: These results indicate that combined AsA and CRet treatment synergistically inhibits EAT cell growth through G2/M arrest and apoptosis induction via H2O2 generation at lower AsA concentrations; this carcinostatic effect cannot be exert ed by AsA alone.

show abstract

Transferencia eléctrica capacitiva (TEC). Técnica no invasiva de hipertermia profunda en el tratamiento de los gliomas cerebrales. Resultados preliminares

Cited by 5 publications

References 13 publications

Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells

Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells

Cytostatic response of HepG2 to 0.57 MHz electric currents mediated by changes in cell cycle control proteins

Synergic Carcinostatic Effects of Ascorbic Acid and Hyperthermia on Ehrlich Ascites Tumor Cell

Contact Info

Product

Resources

About