Transferrin and transferrin receptor expression in intraocular proliferative disease. APAAP-immunolabeling of retinal membranes and ELISA for vitreal transferrin

Weller, Michael; Wiedemann, Peter; Moter, H; Heimann, Klaus

doi:10.1007/bf02172763

Cited by 20 publications

(15 citation statements)

References 34 publications

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“…The presence of transferrin, however, could be confirmed in all specimens, as reported previously [2]. Macrophages were observed in all eyes with traumatic PVR, in one eye with idiopathic PVR, and in none with PDR.…”

Section: Immunocytochemistrysupporting

confidence: 49%

“…Immunochemistry for lactoferrin in preretinal membranes was carried out according to standard procedures [2,9]. After thawing, cryostat sections were refixed in acetone, air-dried, and then incubated with bovine serum albumin (10 mg/ml) to block endogenous protein-binding sites.…”

Section: Methodsmentioning

confidence: 99%

“…Intravitreal levels of transferrin were determined as described previously [2]. The ELISA for intravi treal lactoferrin was performed as a modification of this technique.…”

Section: Methodsmentioning

confidence: 99%

“…At low dilutions of a sample (< 1:10), the high pro tein content contributes to unspecific bind ing. Based on this calculation rationale [2], we can merely state that the normal vitreous does not contain more than 50 pg/1 lactofer rin. A precise value cannot be established with our assay.…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

“…Although the evidence for a role of trans forming growth factor-beta or platelet-de rived growth factor in the development of PID may be compelling, the precise mecha nisms of growth factor action in vitreoretinal proliferation have not been elucidated yet. We have recently shown that the iron trans port protein transferrin is present in physio-logic human vitreous and can be labeled in surgically obtained membrane specimens from patients with PID [2], Transferrin is an essential requirement for cellular growth and mitosis. The expression of cell surface recep tors for transferrin is stimulated by growth factors [3],…”

Section: Introductionmentioning

confidence: 99%

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Iron-Binding Proteins in the Human Vitreous: Lactoferrin and Transferrin in Health and in Proliferative Intraocular Disorders

Weller¹,

Clausen²,

Heimann³

et al. 1990

Ophthalmic Res

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Transferrin, recently detected in preretinal membranes, may contribute to cell proliferation by iron donation for mitosis. We have investigated whether lactoferrin, the second iron-binding protein of the human vitreous, could play a similar role in proliferative intraocular disorders (PID). Using immunochemistry, however, we could not label lactoferrin in surgically obtained membrane specimens from patients with idiopathic proliferative vitreoretinopathy (PVR), traumatic PVR, and proliferative diabetic retinopathy (n = 15). The amounts of both proteins in normal human vitreous, as measured by enzyme-linked immunosorbent assay, were 73.7 ± 6.6 mg/l for transferrin but below 50 μg/l for lactoferrin. Transferrin was also determined in 35 vitreous aspirates from patients with PID. The highest levels were found in idiopathic PVR (846 ± 256 mg/l), followed by proliferative diabetic retinopathy (405 ± 121) and traumatic PVR (197 ± 83 mg/l). A statistically significant difference between the three types of PID and physiologic vitreous, respectively, was not observed. The total vitreal protein, however, was significantly elevated in all three groups of PID.

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Section: Immunocytochemistrysupporting

confidence: 49%

Section: Methodsmentioning

confidence: 99%

“…Intravitreal levels of transferrin were determined as described previously [2]. The ELISA for intravi treal lactoferrin was performed as a modification of this technique.…”

Section: Methodsmentioning

confidence: 99%

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Iron-Binding Proteins in the Human Vitreous: Lactoferrin and Transferrin in Health and in Proliferative Intraocular Disorders

Weller¹,

Clausen²,

Heimann³

et al. 1990

Ophthalmic Res

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Transferrin Receptor Expression by Retinal Pigment Epithelial Cells in Proliferative Vitreoretinopathy

Baudouin¹,

Brignole²,

Gastaud³

1991

Arch Ophthalmol

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The role of growth factors in the embryogenesis and differentiation of the eye

Tripathi

Livingston

et al. 1991

Am. J. Anat.

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The vertebrate eye is composed of a variety of tissues that, embryonically, have their derivation from surface ectoderm, neural ectoderm, neural crest, and mesodermal mesenchyme. During development, these different types of cells are subjected to complex processes of induction and suppressive interactions that bring about their final differentiation and arrangement in the fully formed eye. With the changing concept of ocular development, we present a new perspective on the control of morphogenesis at the cellular and molecular levels by growth factors that include fibroblast growth factors, epidermal growth factor, nerve growth factor, platelet-derived growth factor, transforming growth factors, mesodermal growth factors, transferrin, tumor necrosis factor, neuronotrophic factors, angiogenic factors, and antiangiogenic factors. Growth factors, especially transforming growth factor-beta, have a crucial role in directing the migration and developmental patterns of the cranial neural-crest cells that contribute extensively to the structures of the eye. Some growth factors also exert an effect on the developing ocular tissues by influencing the synthesis and degradation of the extracellular matrix. The mRNAs for the growth factors that are involved in the earliest aspects of the growth and differentiation of the fertilized egg are supplied from maternal sources until embryonic tissues are able to synthesize them. Subsequently, the developing eye tissues are exposed to both endogenous and exogenous growth factors that are derived from nonocular tissues as well as from embryonic fluids and the systemic circulation. The early interaction between the surface head ectoderm and the underlying chordamesoderm confers a lens-forming bias on the ectoderm; later, the optic vesicle elicits the final phase of determination and enhances differentiation by the lens. After the blood-ocular barrier is established, the internal milieu of the eye is controlled by the interactions among the intraocular tissues; only those growth factors that selectively cross the barrier or that are synthesized by the ocular tissues can influence further development and differentiation of the cells. An understanding of the tissue interactions that are regulated by growth factors could clarify the precise mechanism of normal and abnormal ocular development.

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Transferrin and transferrin receptor expression in intraocular proliferative disease. APAAP-immunolabeling of retinal membranes and ELISA for vitreal transferrin

Cited by 20 publications

References 34 publications

Iron-Binding Proteins in the Human Vitreous: Lactoferrin and Transferrin in Health and in Proliferative Intraocular Disorders

Iron-Binding Proteins in the Human Vitreous: Lactoferrin and Transferrin in Health and in Proliferative Intraocular Disorders

Transferrin Receptor Expression by Retinal Pigment Epithelial Cells in Proliferative Vitreoretinopathy

The role of growth factors in the embryogenesis and differentiation of the eye

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