Transferrin mediated solid lipid nanoparticles containing curcumin: Enhanced in vitro anticancer activity by induction of apoptosis

Mulik, Rohit S.; Mönkkönen, Jukka; Juvonen, Risto O.; Mahadik, Kakasaheb R.; Paradkar, Anant

doi:10.1016/j.ijpharm.2010.07.021

Cited by 232 publications

(125 citation statements)

References 44 publications

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“…However, as the concentration increased the drugs presented significant differences in cell viability depending on their potency. CRC was highly potent with only 7% viability (Mulik et al, 2010) at 7g/ml followed by CPT with 9.4% at 200g/ml. As it can be seen 5-FU was the less potent active substance with only 20% cell viability at 400g/ml.…”

Section: Resultsmentioning

confidence: 98%

Inkjet printing of transdermal microneedles for the delivery of anticancer agents

Uddin

Scoutaris

Klepetsanis

et al. 2015

International Journal of Pharmaceutics

159

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A novel inkjet printing technology is introduced as a process to coat metal microneedle arrays with three anticancer agents 5-fluororacil, curcumin and cisplatin for transdermal delivery. The hydrophilic graft copolymer Soluplus® was used as a drug carrier and the coating formulations consisted of drug -polymer solutions at various ratios. A piezoelectric dispenser jetted microdroplets on the microneedle surface to develop uniform, accurate and reproducible coating layers without any material losses. Inkjet printing was found to depend on the nozzle size, the applied voltage (mV) and the duration of the pulse (s). The drug release rates were determined in vitro using Franz type diffusion cells with dermatomed porcine skin. The drug release rates depended on the drug-polymer ratio, the drug lipophilicity and the skin thickness. All drugs presented increased release profiles (750 m skin thickness), which were retarded for 900 m skin thickness. Soluplus assisted the drug release especially for the water insoluble curcumin and cisplatin due to its solubilizing capacity. Inkjet printing was proved an effective technology for coating of metal microneedles which can then be used for further transdermal drug delivery applications.

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Section: Resultsmentioning

confidence: 98%

Inkjet printing of transdermal microneedles for the delivery of anticancer agents

Uddin

Scoutaris

Klepetsanis

et al. 2015

International Journal of Pharmaceutics

159

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“…In vitro cytotoxicity study in U-87 mg human glioblastoma cells line Cytotoxicity was determined by the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay as previously described (Mulik et al, 2010). The U-87 MG cells were grown on Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS at 37 C. The cell count was determined using a hemocytometer.…”

Section: Targeting Efficiency By Intranasal Administrationmentioning

confidence: 99%

Docosahexaenoic acid–mediated, targeted and sustained brain delivery of curcumin microemulsion

Shinde

Devarajan

2017

Drug Delivery

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We disclose microemulsions (ME) of curcumin (CUR) with docosahexaenoic acid (DHA)-rich oil (CUR DHA ME) for targeted delivery to the brain. MEs of CUR (5 mg/mL) with and without DHArich oil (CUR Capmul ME) suitable for intravenous and intranasal administration exhibited negative zeta potential, globule size 520 nm and good stability. Following intravenous delivery MEs exhibited high brain concentration with CUR DHA ME exhibiting a 2.8-fold higher C max than CUR solution. Furthermore, high and sustained concentration was demonstrated even at 24 h, which was 8-and 2-fold higher than CUR solution and CUR Capmul ME, respectively. Brain concentrations following intranasal administration were, however, substantially higher as evident from higher C max and AUC and sustained compared to corresponding intravenous formulations signifying nose to brain targeting. The high brain concentration of CUR DHA ME is ascribed to the targeting efficiency enabled by DHA-mediated transport across the blood-brain barrier (BBB). Histopathological and nasal toxicity confirmed safety of the MEs. Concentrationdependent cytotoxicity in vitro, on human glioblastoma U-87MG cell line was observed with CUR DHA MEs and with the blank DHA ME, implying anticancer potential of DHA. The dramatically low IC 50 value of CUR DHA ME (3.755 ± 0.24 ng/mL) is therefore attributed to the synergistic effect of CUR and DHA in the ME. The CUR concentration achieved with CUR DHA ME at 24 h which translated to 466-fold(intranasal) and 421-fold (intravenous) the IC 50 value in the U-87MG cell line suggests great promise of CUR DHA ME for therapy of brain cancer by both routes.

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“…40 The anticancer activity of transferring-conjugated solid-lipid NPs of curcumin on the MCF-7 cell line is also evident. 42 Several such studies suggested the potential of targeted therapeutic nanoparticles (TNPs) as effective anticancer drug-delivery systems. 2,22,25,40,43 In an in vivo animal study, targeted TNP-delivered paclitaxel was mainly located in tumor cells, while nontargeted TNP-delivered paclitaxel was detected at intercellular level.…”

Section: Targeting Tumor Cellsmentioning

confidence: 99%

“…128 Recently, several studies reported the use of SLNs as tumor-targeted drug-delivery systems. 42,57,129,130 SLNs loaded with doxorubicin were studied in Pgp-overexpressing MCF-7/ADR cell lines. 57 Efficiently enhanced apoptotic cell death via higher accumulation of doxorubicin by SLNs in cancer cells was also reported.…”

Section: Solid-lipid Nanoparticles As Targeted Drug-delivery Systemmentioning

confidence: 99%

“…57 Targeted delivery of a potent anticancer compound, namely curcumin, incorporated with transferrin-mediated SLNs has also been reported in MCF-7 breast cancer cells. 42 More recently, high-pressure homogenized emodin-loaded SLNs gave positive antitumor activity in vitro. 129 Moreover, cationic lipid-bound oligonucleotide-loaded SLNs are also reported as a potential new approach for carrying antimiRNA inhibitors for cancer therapy.…”

Section: Solid-lipid Nanoparticles As Targeted Drug-delivery Systemmentioning

confidence: 99%

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Nanotechnology-based approaches in anticancer research

Jabir¹,

Tabrez²,

Ashraf³

et al. 2012

IJN

149

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Cancer is a highly complex disease to understand, because it entails multiple cellular physiological systems. The most common cancer treatments are restricted to chemotherapy, radiation and surgery. Moreover, the early recognition and treatment of cancer remains a technological bottleneck. There is an urgent need to develop new and innovative technologies that could help to delineate tumor margins, identify residual tumor cells and micrometastases, and determine whether a tumor has been completely removed or not. Nanotechnology has witnessed significant progress in the past few decades, and its effect is widespread nowadays in every field. Nanoparticles can be modified in numerous ways to prolong circulation, enhance drug localization, increase drug efficacy, and potentially decrease chances of multidrug resistance by the use of nanotechnology. Recently, research in the field of cancer nanotechnology has made remarkable advances. The present review summarizes the application of various nanotechnology-based approaches towards the diagnostics and therapeutics of cancer.

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Transferrin mediated solid lipid nanoparticles containing curcumin: Enhanced in vitro anticancer activity by induction of apoptosis

Cited by 232 publications

References 44 publications

Inkjet printing of transdermal microneedles for the delivery of anticancer agents

Inkjet printing of transdermal microneedles for the delivery of anticancer agents

Docosahexaenoic acid–mediated, targeted and sustained brain delivery of curcumin microemulsion

Nanotechnology-based approaches in anticancer research

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