Transferrin receptor in oral tumors

Miyamoto, Takao; Tanaka, Nobuyuki; Eishi, Yoshinobu; Amagasa, Teruo

doi:10.1016/s0901-5027(05)80039-6

Cited by 36 publications

(18 citation statements)

References 5 publications

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“…Since malignant oral squamous cells overexpress transferrin receptors as compared to non-malignant cells [21,22], we investigated whether treatment of tongue cancer cells with GCV would induce significant cell death following delivery of HSVtk gene by Tf-lipoplexes. The effect of critical parameters, such as size and lipid/DNA stoichiometry of the complexes on their biological activity, was assessed in order to establish the most efficient transfection conditions for these cells.…”

Section: Introductionmentioning

confidence: 99%

Transfection of oral cancer cells mediated by transferrin-associated lipoplexes: Mechanisms of cell death induced by herpes simplex virus thymidine kinase/ganciclovir therapy

Neves¹,

Sarmento‐Ribeiro

Simões

et al. 2006

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The Herpes Simplex Virus thymidine kinase (HSV-tk) suicide gene/ganciclovir (GCV) approach has been used for the treatment of a variety of cancers. The purpose of the present study was to evaluate the cytotoxic effect of ganciclovir in oral squamous cancer cells, previously transfected with HSV-tk gene delivered by transferrin-associated complexes (Tf-lipoplexes), as well as to investigate the mechanisms involved in the bystander effect and in the process of cell death. The delivery of HSV-tk gene to the oral cancer cells, HSC-3 and SCC-7, mediated by Tf-lipoplexes followed by ganciclovir treatment resulted in essentially 100% cytotoxicity, the observed toxic effect being dependent both on GCV dose and incubation time. Cell death was shown to occur mainly by an apoptotic process. Different experimental approaches demonstrated that the observed cytotoxicity was mainly due to diffusion of the toxic agent into neighbouring, non-transfected cells, via gap junctions. Preliminary in vivo studies in a murine model for oral squamous cell carcinoma have shown a significant inhibition of tumor growth upon injection of Tf-lipoplexes carrying HSV-tk followed by intraperitonal injection of GCV, as compared to controls.

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Section: Introductionmentioning

confidence: 99%

Transfection of oral cancer cells mediated by transferrin-associated lipoplexes: Mechanisms of cell death induced by herpes simplex virus thymidine kinase/ganciclovir therapy

Neves¹,

Sarmento‐Ribeiro

Simões

et al. 2006

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…We have previously reported that the addition of the transferrin (Tf) ligand to a cationic liposome com plex resulted in an in vitro transfection efficiency of 70-80% in squamous cell carcinoma of the head and neck (SCCHN) as compared with only 5-20% transfection by the cationic liposome com plex without Tf (L. . Transferrin receptor (TfR) levels are known to be elevated in various types of cancer cells including breast (Inoue et al, 1993) and prostate cancers (Keer et al, 1990), even those prostate cancer cell lines derived from human lym ph node and bone metastases (Grayhack et al, 1979;Chackal-Roy et al, 1989;Rossi and Zetter, 1992), as well as squam ous cell carcinomas (Thorstensen and Romsio, 1993;Miyamoto et al, 1994), and correlate with the aggressive or proliferative ability of tumor cells (Elliott et al, 1993).…”

Section: Introduction Wmentioning

confidence: 99%

Transferrin-Liposome-Mediated Systemic p53 Gene Therapy in Combination with Radiation Results in Regression of Human Head and Neck Cancer Xenografts

Pirollo²,

Tang³

et al. 1999

Human Gene Therapy

207

176

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“…Similarly, cytokeratin 16, a marker for high cell turnover characteristic for both keratinizing and nonkeratinizing types of epithelium, was only detected in a minority of cells (Figure 4C). The transferrin receptor, a protein characteristic of proliferating cells from the parabasal and basal layers of the epidermis (20), was present in a high proportion of cells (Figure 4D). Another parabasal/basal layer marker, integrin 1 , was also expressed by many mouthwash cells (Figure 4E), indicating a parabasal origin.…”

Section: Resultsmentioning

confidence: 99%

Fresh and cultured buccal cells as a source of mRNA and protein for molecular analysis

Michalczyk

Varigos

Smith³

et al. 2004

BioTechniques

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We developed a method for obtaining viable buccal cells from mouthwash samples for use as a source of mRNA and protein. Immunofluorescent analysis showed that most cells were derived from nonkeratinized parabasal epithelia, with a minor proportion of proliferative cells. Gene expression was detected in buccal cells using reverse transcription PCR, Western blot analysis, and immunofluorescence. Using a keratinocyte-specific medium, buccal cells could be cultured on Matrigel-coated permeable filters for up to 2 weeks while maintaining the expression of some epithelial-specific markers, including cytokeratin 13, cytokeratin 10, transferrin receptor, and beta-integrin. The basal marker cytokeratin 14 and Ki67, an indicator of cellular proliferation, were detected in a few cells. We show that buccal cells can be obtained from a noninvasive procedure for use as a source of material for biochemical analyses. A population of the buccal cells can be maintained in culture for up to 2 weeks using keratinocyte-specific medium in combination with extracellular matrix.

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Transferrin receptor in oral tumors

Cited by 36 publications

References 5 publications

Transfection of oral cancer cells mediated by transferrin-associated lipoplexes: Mechanisms of cell death induced by herpes simplex virus thymidine kinase/ganciclovir therapy

Transfection of oral cancer cells mediated by transferrin-associated lipoplexes: Mechanisms of cell death induced by herpes simplex virus thymidine kinase/ganciclovir therapy

Transferrin-Liposome-Mediated Systemic p53 Gene Therapy in Combination with Radiation Results in Regression of Human Head and Neck Cancer Xenografts

Fresh and cultured buccal cells as a source of mRNA and protein for molecular analysis

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