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300/300 words) 35Extracting biological signals from non-linear, dynamic and stochastic experimental data can be 36 challenging, especially when the signal is non-stationary. Many currently available methods make 37 assumptions about the data structure (e.g., signal is periodic, sufficient recording time) and modify 38 the raw data in pre-processing using filters and/or transformations. With an agnostic approach to 39 biological data analysis as a goal, we implemented a signal detection algorithm in Python that 40 quantifies the dimensional properties of waveform deviations from baseline via a running fit 41 function. We call the resulting free program frequency-independent biological signal identification 42 (FIBSI). We demonstrate the utility of FIBSI on two disparate types of experimental data: in vitro 43 whole-cell current-clamp electrophysiological recordings of rodent sensory neurons (i.e., 44 nociceptors) and in vivo fluorescence image time-lapse movies capturing gastrointestinal motility 45 in larval zebrafish. In rodent nociceptors, depolarizing fluctuations in membrane potential are 46 irregular in shape and difficult to distinguish from noise. Using FIBSI, we determined that 47 Author Summary (172/200 words) 60Biologists increasingly work with large, complex experimental datasets. Those datasets often 61 encode biologically meaningful signals along with background noise that is recorded along with 62 the biological data during experiments. Background noise masks the real signal but originates 63 from other sources, for example from the equipment used to perform the measurements or 64 environmental disturbances. When it comes to analyzing the data, distinguishing between the real 65 biological signals and the background noise can be very challenging. Many existing programs 66 designed to help scientists with this problem are either difficult to use, not freely available, or only 67 appropriate to use on very specific types of datasets. The research presented here embodies our 68 goal of helping others to analyze their data by employing a powerful but novice-friendly program 69 that describes multiple features of biological activity in its raw form without abstract 70 transformations. We show the program's applicability using two different kinds of biological activity 71 measured in our labs. It is our hope that this will help others to analyze complex datasets more 72 easily, thoroughly, and rigorously. 73 157
300/300 words) 35Extracting biological signals from non-linear, dynamic and stochastic experimental data can be 36 challenging, especially when the signal is non-stationary. Many currently available methods make 37 assumptions about the data structure (e.g., signal is periodic, sufficient recording time) and modify 38 the raw data in pre-processing using filters and/or transformations. With an agnostic approach to 39 biological data analysis as a goal, we implemented a signal detection algorithm in Python that 40 quantifies the dimensional properties of waveform deviations from baseline via a running fit 41 function. We call the resulting free program frequency-independent biological signal identification 42 (FIBSI). We demonstrate the utility of FIBSI on two disparate types of experimental data: in vitro 43 whole-cell current-clamp electrophysiological recordings of rodent sensory neurons (i.e., 44 nociceptors) and in vivo fluorescence image time-lapse movies capturing gastrointestinal motility 45 in larval zebrafish. In rodent nociceptors, depolarizing fluctuations in membrane potential are 46 irregular in shape and difficult to distinguish from noise. Using FIBSI, we determined that 47 Author Summary (172/200 words) 60Biologists increasingly work with large, complex experimental datasets. Those datasets often 61 encode biologically meaningful signals along with background noise that is recorded along with 62 the biological data during experiments. Background noise masks the real signal but originates 63 from other sources, for example from the equipment used to perform the measurements or 64 environmental disturbances. When it comes to analyzing the data, distinguishing between the real 65 biological signals and the background noise can be very challenging. Many existing programs 66 designed to help scientists with this problem are either difficult to use, not freely available, or only 67 appropriate to use on very specific types of datasets. The research presented here embodies our 68 goal of helping others to analyze their data by employing a powerful but novice-friendly program 69 that describes multiple features of biological activity in its raw form without abstract 70 transformations. We show the program's applicability using two different kinds of biological activity 71 measured in our labs. It is our hope that this will help others to analyze complex datasets more 72 easily, thoroughly, and rigorously. 73 157
EEG is a common and safe test that uses small electrodes to record electrical signals from the brain. It has a broad range of applications in medical diagnosis, including diagnosis of epileptic seizure, Alzheimer's, brain tumors, head injury, sleep disorders, stroke, and other seizure and neurological disorders. EEG can also be used to help diagnose death in people who are in a persistent coma. The use of digital signal processing and machine learning to improve EEG analysis for medical diagnosis has gained traction in recent years. This is because EEG visual analysis can be complex and time-consuming, as it mostly involves high dimensions and consists of large datasets. The development of novel sensors for EEG recording, digital signal processing algorithms, feature engineering, and detection algorithms increases the need for efficient diagnostic systems. An extensive review of the recent approaches for EEG preprocessing, extraction of features, and diagnosis of brain disorders is provided. In this paper, the main focus is to identify reliable algorithms for preprocessing, feature engineering, and classification of EEG, applied to medical healthcare and diagnosis, providing practitioners with insights into the most effective strategies, as well as potential future directions for improving accuracy of the automatic diagnostic systems. The study of reliable feature extraction and classification algorithms is crucial for a more accurate analysis of EEG signals. This paper can provide valuable information to researchers and practitioners working in the fields of EEG analysis and machine learning, as it provides a summary of recent developments and highlights key areas for future research. This paper can help researchers and clinicians to stay up-to-date on the latest developments in this field.
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