TRANSFORMATION INDUCED BY SIMIAN VIRUS 40 IN HUMAN RENAL CELL CULTURES, I. MORPHOLOGY AND GROWTH CHARACTERISTICS
            <sup>*</sup>

Shein, Harvey M.; Enders, John F.

doi:10.1073/pnas.48.7.1164

Cited by 189 publications

(51 citation statements)

References 21 publications

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“…Subsequent experimentation determined that formaldehyde treatment, as was performed to inactivate the poliovirus in the vaccine, did not completely inactivate all SV40 virions [4]. Studies confirmed the ability of SV40 to induce tumors in vivo in neonatal hamsters [5][6][7][8] and to transform human cell lines in vitro [9,10]. Subcutaneous tumor nodules could also be demonstrated in terminally ill human patients injected with SV40 transformed cells [11].…”

Section: Early History Of Sv40mentioning

confidence: 99%

SV40 association with human malignancies and mechanisms of tumor immunity by large tumor antigen

Lowe

Shearer

Jumper

et al. 2007

Cell. Mol. Life Sci.

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SV40 was discovered as a contaminate of poliovirus vaccine lots distributed to millions of individuals in the United States between 1955 and 1963 while contaminated vaccine batches were later circulated worldwide. After SV40 was observed to cause in vitro animal and human cell transformations and in vivo tumor formations in animals, the search for a connection between the virus and human malignancies has continued to the present day. Different molecular methods have been used to detect SV40 gene products in a variety of human cancers, though SV40 causality in these tumor types has yet to be established. These data, however, are not without controversial issues related to inconclusive SV40 serological and epidemiological evidence alongside tools and methodologies that may contribute to false-positive results in human specimens. This review will also explore how vaccination against SV40 protein products may be used to help prevent and treat individuals with SV40-expressing cancers.

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Section: Early History Of Sv40mentioning

confidence: 99%

SV40 association with human malignancies and mechanisms of tumor immunity by large tumor antigen

Lowe

Shearer

Jumper

et al. 2007

Cell. Mol. Life Sci.

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“…In addition, the detection of SV40 in tumors in children too young to have received the contaminated poliovirus vaccine has been interpreted by some investigators as evidence that the virus is circulating in human populations and transmitted from person to person (3). SV40-induced carcinogenesis is biologically plausible because the virus encodes an oncoprotein (20), can transform human cells in vitro (24), and induces tumors in rodents (7,13). However, there are also reasons for skepticism concerning a role for SV40 in human cancers.…”

mentioning

confidence: 99%

Serological Cross-Reactivities between Antibodies to Simian Virus 40, BK Virus, and JC Virus Assessed by Virus-Like-Particle-Based Enzyme Immunoassays

Viscidi

Rollison

Viscidi

et al. 2003

Clin Vaccine Immunol

133

136

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Enzyme immunoassays (EIAs) for detection of serum antibodies to simian virus 40 (SV40), BK virus (BKV), and JC virus (JCV) were developed by using virus-like-particles (VLPs) produced in insect cells from recombinant baculoviruses expressing the VP1 protein of the respective virus. Rhesus macaque sera with neutralizing antibodies to SV40 showed a high level of reactivity in the SV40 VLP-based EIA, and these sera also showed lower levels of reactivity in the BKV and JCV VLP-based EIAs. Rhesus macaque sera negative for neutralizing antibodies to SV40 were negative in all three EIAs. Competitive binding assays showed that SV40 VLPs inhibited BKV reactivity. In rhesus macaque sera, high optical density (OD) values for antibodies to SV40 VLPs were correlated with high OD values for antibodies to BKV but not with high OD values for antibodies to JCV VLPs. Human sera with neutralizing antibodies to SV40 were more reactive to SV40 VLPs than human sera without neutralizing antibodies to SV40. The greater SV40 reactivities of human sera were correlated with greater reactivities to BKV VLPs but not JCV VLPs. These data suggest that cross-reactivity with BKV antibodies may account for part of the low-level SV40 reactivity seen in human sera. With their greater versatility and their suitability for large-scale testing, the VLP-based EIAs for SV40, BKV, and JCV are likely to contribute to a better understanding of the biology of these viruses.

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“…SIMIAN virus SV40 has been shown to transform cells from a variety of animals and from man (Rabson and Kirschstein, 1962;Stein and Enders, 1962;Jensen, Koprowski and Ponten, 1963;Black and Rowe, 1963;Gaffney et al, 1970). The transformation of human cells has been reported for in vitro cultures established from a number of different tissues (Rabson and Kirshstein, 1962;Jensen et al, 1963;Fogh, 1966;Nishida, 1970); in particular, cell cultures derived from small skin samples have been transformed by SV40 virus and this transformation can be accurately quantitated (Aaronson and Todaro, 1968;Aaronson and Martin, 1970;Potter, Potter and Oxford, 1970).…”

mentioning

confidence: 99%

Transformation of human cells by SV40 virus

Potter¹,

Potter²

1975

Br J Cancer

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Fibroblast cultures were prepared from skin biopsies from 29 patients and tested for their susceptibility to transformation by simian virus SV40. Cells with a normal chromosome complement showed a mean transformation frequency of 25/106 cells but for cells from a single patient with Fanconi's anaemia, the value was 152/106 cells. An increased susceptibility to transformation was observed for cells from 6 patients with Down's syndrome 3 patients with trisomy 18, a patient with trisomy 18 for 5% of cells and a patient with trisomy 13. No increased susceptibility to transformation was found for cells with a chromosome complement of XO, XXY, XX/XX + 8, XX + partial 15q or XX + 9p. The susceptiability to transformation was related to susceptibility to SV40 virus infection, as measured by the number of infected cells which contained SV40 virus induced T antigen. This latter test was technically easier to perform and could serve to detect persons of increased susceptiability to transformation, since this may indicate an increased risk of natural malignant disease.

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TRANSFORMATION INDUCED BY SIMIAN VIRUS 40 IN HUMAN RENAL CELL CULTURES, I. MORPHOLOGY AND GROWTH CHARACTERISTICS ^*

Cited by 189 publications

References 21 publications

SV40 association with human malignancies and mechanisms of tumor immunity by large tumor antigen

SV40 association with human malignancies and mechanisms of tumor immunity by large tumor antigen

Serological Cross-Reactivities between Antibodies to Simian Virus 40, BK Virus, and JC Virus Assessed by Virus-Like-Particle-Based Enzyme Immunoassays

Transformation of human cells by SV40 virus

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TRANSFORMATION INDUCED BY SIMIAN VIRUS 40 IN HUMAN RENAL CELL CULTURES, I. MORPHOLOGY AND GROWTH CHARACTERISTICS *

Cited by 189 publications

References 21 publications

SV40 association with human malignancies and mechanisms of tumor immunity by large tumor antigen

SV40 association with human malignancies and mechanisms of tumor immunity by large tumor antigen

Serological Cross-Reactivities between Antibodies to Simian Virus 40, BK Virus, and JC Virus Assessed by Virus-Like-Particle-Based Enzyme Immunoassays

Transformation of human cells by SV40 virus

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TRANSFORMATION INDUCED BY SIMIAN VIRUS 40 IN HUMAN RENAL CELL CULTURES, I. MORPHOLOGY AND GROWTH CHARACTERISTICS ^*