Transformation mechanism of methylphosphonate to methane by Burkholderia sp: Insight from multi-labeled water isotope probing and transcriptomic

Li, Junhong; Liu, Houquan; Liu, Zeqin; Zhang, Xianhua; Blake, Ruth E.; Huang, Zhiyong; Cai, Meifeng; Wang, Fei; Yu, Chan

doi:10.1016/j.envres.2022.114970

Cited by 4 publications

(1 citation statement)

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“…The results showed that the nirB (3.6%), narG (5.5%), narH (2.5%), and nasA (2.8%) genes related to the nitrate cycle and the ppk (6.5%), pstA (4.3%), pstB (4.2%), pstC (4.4%), and pstS (6.4%) genes related to the phosphate cycle were highly expressed in all samples ( Supplementary Figure S6 ), and most (nitrate: nirB / narG / narH/nasA ; phosphorus: pstA / pstB / pstC / pstS ) were expressed significantly higher in the lake water than in the river water and sediment ( Figure 4 ). These genes are closely related to nitrate reduction, phosphate absorption, and transport (Yu et al, 2018 ; Li et al, 2023 ; Liu et al, 2023 ). On this basis, the main microorganisms contributing these genes with high expression in lakes were identified in this study through the combination of species annotation and gene abundance calculation ( Supplementary Figure S7 ; the detailed descriptions are shown in the Supplementary Text description of the analysis of key microorganisms contributing nitrogen and phosphorus genes).…”

Section: Resultsmentioning

confidence: 99%

Unraveling the rate-limiting step in microorganisms' mediation of denitrification and phosphorus absorption/transport processes in a highly regulated river-lake system

Ding,

Yang,

Liu

et al. 2023

Front. Microbiol.

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River–lake ecosystems are indispensable hubs for water transfers and flow regulation engineering, which have frequent and complex artificial hydrological regulation processes, and the water quality is often unstable. Microorganisms usually affect these systems by driving the nutrient cycling process. Thus, understanding the key biochemical rate-limiting steps under highly regulated conditions was critical for the water quality stability of river–lake ecosystems. This study investigated how the key microorganisms and genes involving nitrogen and phosphorus cycling contributed to the stability of water by combining 16S rRNA and metagenomic sequencing using the Dongping river–lake system as the case study. The results showed that nitrogen and phosphorus concentrations were significantly lower in lake zones than in river inflow and outflow zones (p < 0.05). Pseudomonas, Acinetobacter, and Microbacterium were the key microorganisms associated with nitrate and phosphate removal. These microorganisms contributed to key genes that promote denitrification (nirB/narG/narH/nasA) and phosphorus absorption and transport (pstA/pstB/pstC/pstS). Partial least squares path modeling (PLS-PM) revealed that environmental factors (especially flow velocity and COD concentration) have a significant negative effect on the key microbial abundance (p < 0.001). Our study provides theoretical support for the effective management and protection of water transfer and the regulation function of the river–lake system.

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Section: Resultsmentioning

confidence: 99%