Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans

Buxton, Frank P.; Gwynne, David I.; Davies, R. Wayne

doi:10.1016/0378-1119(85)90274-4

Cited by 106 publications

(27 citation statements)

References 17 publications

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“…This agrees with the results of transformation experiments on A. nidulans 13 ,26) and A. niger, 9,27) but differs from those with the yeast, S. cerevisiae, for which integration events were found to be strongly dependent on homology.2S) Nevertheless, it cannot be excluded that homologous sequences are required for efficient integration into the genome, resulting in a higher frequency of transformation than that obtained in this study. Therefore, it is necessary for improvement of the transformation frequency to construct a hybrid plasmid, such as pRBMl, by the addition of DNA sequences homologous to the host genome.…”

Section: Discussionsupporting

confidence: 89%

“…The frequency in this study is much lower than that obtained for A. nidulans but approximately the same as that in the transformation experiment with A. niger using a plasmid containing A. nidulans argB gene. 9 ) Three of the 7 transformants, showing vigorous growth on successive transfers to MM, were selected for detailed analysis.…”

Section: Transformation Of An a Oryzae Arginineauxotrophic Mutant Wimentioning

confidence: 99%

“…3.3) contains no introns 5 ) and allows the complementation of the arginine-auxotrophic mutation of E. coli. 6 ) Saccharomyces cerevisiae,7) Neurospora crassa 8 ) and A. niger 9 ) as well as A. nidulans. 1O -13 ) We report here the successful transformation of an A. oryzae arginine-auxotrophic mutant, using a plasmid which contained the argB gene of A. nidulans.…”

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confidence: 99%

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Integrative Transformation ofAspergillus oryzaewith a Plasmid Containing theAspergillus nidulans argBGene

Gomi¹,

Iimura²,

Hara³

1987

Agricultural and Biological Chemistry

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Section: Discussionsupporting

confidence: 89%

Section: Transformation Of An a Oryzae Arginineauxotrophic Mutant Wimentioning

confidence: 99%

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confidence: 99%

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Integrative Transformation ofAspergillus oryzaewith a Plasmid Containing theAspergillus nidulans argBGene

Gomi¹,

Iimura²,

Hara³

1987

Agricultural and Biological Chemistry

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“…For decades they have been commonly exploited as commercial production organisms for a variety of enzymes. With the development of transformation systems for these industrially important members of the genus (Buxton et al, 1985;Kelly & Hynes, 1985;van Hartingsveldt et al, 1987;Iimura et al, 1987;Unkles et al, 1989), the expression of large quantities of heterologous proteins seemed within reach as well. And indeed, nowadays Aspergillus species dominate the list of host organisms for the commercial production of enzymes from fungal origin (according to the Association of Manufacturers and Formulators of Enzyme Products at www.amfep.org).…”

Section: Introductionmentioning

confidence: 99%

The effect of environmental conditions on extracellular protease activity in controlled fermentations of Aspergillus niger

et al. 2009

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Proteolytic degradation by host proteases is one of the key issues in the application of filamentous fungi for non-fungal protein production. In this study the influence of several environmental factors on the production of extracellular proteases of Aspergillus niger was investigated systematically in controlled batch cultures. Of all factors investigated in a series of initial screening experiments, culture pH and nitrogen concentration in particular strongly affected extracellular protease activities. For instance, at a culture pH of 4, protease activity was higher than at pH 5, and protease activity increased with increasing concentrations of ammonium as nitrogen source. Interestingly, an interdependence was observed for several of the factors studied. These possible interaction effects were investigated further using a full factorial experimental design. Amongst others, the results showed a clear interaction effect between nitrogen source and nitrogen concentration. Based on the observed interactions, the selection of environmental factors to reduce protease activity is not straightforward, as unexpected antagonistic or synergistic effects occur. Furthermore, not only were the effects of the process parameters on maximum protease activity investigated, but five other protease-related phenotypes were studied as well, such as maximum specific protease activity and maximum protease productivity. There were significant differences in the effect of the environmental parameters on the various protease-related phenotypes. For instance, pH significantly affected final levels of protease activity, but not protease productivity. The results obtained in this study are important for the optimization of A. niger for protein production.

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“…As for A. nidulans and A. oryzae , molecular transformation followed a natural path of complementation of auxotrophic mutants (Buxton et al 1985) and, ultimately, selection of Hygromycin resistant colonies at rates of 5–20 transformants per microgram of transforming DNA (Punt et al 1987). Gene manipulation using alteration of genes within the natural genome has allowed this organism to be utilised in food and fibre production and modification.…”

Section: Aspergillus Speciesmentioning

confidence: 99%

Diverse data supports the transition of filamentous fungal model organisms into the post-genomics era

McCluskey

Baker

2017

Mycology

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Filamentous fungi have been important as model organisms since the beginning of modern biological inquiry and have benefitted from open data since the earliest genetic maps were shared. From early origins in simple Mendelian genetics of mating types, parasexual genetics of colony colour, and the foundational demonstration of the segregation of a nutritional requirement, the contribution of research systems utilising filamentous fungi has spanned the biochemical genetics era, through the molecular genetics era, and now are at the very foundation of diverse omics approaches to research and development. Fungal model organisms have come from most major taxonomic groups although Ascomycete filamentous fungi have seen the most major sustained effort. In addition to the published material about filamentous fungi, shared molecular tools have found application in every area of fungal biology. Similarly, shared data has contributed to the success of model systems. The scale of data supporting research with filamentous fungi has grown by 10 to 12 orders of magnitude. From genetic to molecular maps, expression databases, and finally genome resources, the open and collaborative nature of the research communities has assured that the rising tide of data has lifted all of the research systems together.

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Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans

Cited by 106 publications

References 17 publications

Integrative Transformation ofAspergillus oryzaewith a Plasmid Containing theAspergillus nidulans argBGene

Integrative Transformation ofAspergillus oryzaewith a Plasmid Containing theAspergillus nidulans argBGene

The effect of environmental conditions on extracellular protease activity in controlled fermentations of Aspergillus niger

Diverse data supports the transition of filamentous fungal model organisms into the post-genomics era

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