Transformation of C19-steroids and testosterone production by sterol-transforming strains of Mycobacterium spp.

Egorova, Olga V.; Nikolayeva, Vera M.; Sukhodolskaya, G. V.; Donova, Marina V.

doi:10.1016/j.molcatb.2008.09.003

Cited by 36 publications

(30 citation statements)

References 25 publications

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“…and ergosterol (98.5 mg) 6.1% to dry wt. Egorova et al (2009) reported that the formation of testosterone from androstadiendione by sterol-transforming strains of Mycobacterium spp reached maximum level of 0.21 mM (6%) at neutral pH, and decreased at the acid or basic pH values, which clearly correlates with the results obtained in the present study.…”

Section: Resultssupporting

confidence: 92%

Finger millet (Eleusine coracana) — an economically viable source for antihypercholesterolemic metabolites production by Monascus purpureus

Venkateswaran

Vijayalakshmi

2010

J Food Sci Technol

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Rice, parboiled rice, finger millet, germinated finger millet, broken wheat, njavara (medicinal rice), sorghum and maize were used as substrates for solid state fermentation of Monascus purpureus at 28°C for 7 days using 2% seed medium as inoculum for the production of its metabolites. The fungus exhibited good growth in all the substrates. The fermented substrates were dried at 45°C and analysed for antihypercholesterolemic metabolite statins by standardized HPLC method and dietary sterol contents by spectrophotometric method using reference standards of statin (pravastatin and lovastatin) and cholesterol, respectively. Germinated finger millet yielded higher total statin production of 5.2 g/kg dry wt with pravastatin and lovastatin content of 4.9 and 0.37 g/kg dry wt respectively than other substrates which range from 1.04-4.41 g/kg. In addition to statin, monascus fermented germinated finger millet yielded dietary sterol of 0.053 g/kg dry wt which is 7.6 folds higher than the control. The value addition of finger millet by germination and fermentation with Monascus purpureus provides scope for development of functional food.

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Section: Resultssupporting

confidence: 92%

Finger millet (Eleusine coracana) — an economically viable source for antihypercholesterolemic metabolites production by Monascus purpureus

Venkateswaran

Vijayalakshmi

2010

J Food Sci Technol

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“…the free and conjugated forms (glucuronides and sulfates) (Mota et al, 2014). When entering the pond of F3, some residual conjugated steroids are likely deconjugated into free forms (Gomes et al, 2009) or converted from some other steroids with similar molecular structures (Egorova et al, 2009;Jenkins et al, 2004). Ten synthetic steroids were detected in water samples of the aquaculture farms (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Steroid bioaccumulation profiles in typical freshwater aquaculture environments of South China and their human health risks via fish consumption

Liu

et al. 2017

Environmental Pollution

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“…An important pharmaceutical androgen—testosterone—can also be the product of microbiological transformation of sterols. Testosterone is obtained from the AD(D) either by a four-step chemical synthesis, or using microbial 17β-reduction by yeasts or fungi [55]. Microbial conversion of sterols to testosterone by using the strains capable of both sterol side chain cleavage and 17β-reduction of AD allows formation of testosterone in one biotechnological step.…”

Section: Production Of Optically Active Compoundsmentioning

confidence: 99%

“…The strain of Mycobacterium sp. VKM Ac-1815D, capable of sterol side chain degradation and expressing 17β-hydroxysteroid dehydrogenase activity, converted β-sitosterol (5 g/L) to testosterone with 50%–55% molar yield [55]. …”

Section: Production Of Optically Active Compoundsmentioning

confidence: 99%

Biotransformations Utilizing β-Oxidation Cycle Reactions in the Synthesis of Natural Compounds and Medicines

Œwizdor

Panek

Milecka-Tronina

et al. 2012

IJMS

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β-Oxidation cycle reactions, which are key stages in the metabolism of fatty acids in eucaryotic cells and in processes with a significant role in the degradation of acids used by microbes as a carbon source, have also found application in biotransformations. One of the major advantages of biotransformations based on the β-oxidation cycle is the possibility to transform a substrate in a series of reactions catalyzed by a number of enzymes. It allows the use of sterols as a substrate base in the production of natural steroid compounds and their analogues. This route also leads to biologically active compounds of therapeutic significance. Transformations of natural substrates via β-oxidation are the core part of the synthetic routes of natural flavors used as food additives. Stereoselectivity of the enzymes catalyzing the stages of dehydrogenation and addition of a water molecule to the double bond also finds application in the synthesis of chiral biologically active compounds, including medicines. Recent advances in genetic, metabolic engineering, methods for the enhancement of bioprocess productivity and the selectivity of target reactions are also described.

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Transformation of C19-steroids and testosterone production by sterol-transforming strains of Mycobacterium spp.

Cited by 36 publications

References 25 publications

Finger millet (Eleusine coracana) — an economically viable source for antihypercholesterolemic metabolites production by Monascus purpureus

Finger millet (Eleusine coracana) — an economically viable source for antihypercholesterolemic metabolites production by Monascus purpureus

Steroid bioaccumulation profiles in typical freshwater aquaculture environments of South China and their human health risks via fish consumption

Biotransformations Utilizing β-Oxidation Cycle Reactions in the Synthesis of Natural Compounds and Medicines

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