The article actualizes and discusses the formation and development of the ecosystem of innovative education in the region, an attempt is made to substantiate such an ecosystem. The purpose of the study, the results of which are presented in the article, was to substantiate the methodology of the ecosystem approach in relation to the consideration of innovative education systems, the analysis of internal and external factors that influence innovation processes. The article notes that in the ecosystem of innovative education in the region, its subjects actively interact with any other subject in their joint innovation activities. The ecosystem of innovative education in the region is interpreted and refers to an integrated environment where all subjects (managers, teachers, methodologists, specialists in the field of IT-technologies, students, representatives of science) interact with each other, as well as with a variety of innovative products, technologies, methods learning and other elements of the educational ecosystem. Moreover, instead of exchanging energy between components (as is customary in natural ecosystems), information is exchanged in an educational ecosystem, especially an innovative one. In the logic of the ecosystem approach, innovative models of the cross-multidimensional organization of the educational process are proposed, when the educational environment is complemented, intersected and interacts with other spaces and environments that have an appropriate educational and developmental potential. The article presents and discusses the results of a study with the participation of forty-six managerial and pedagogical workers, aimed at identifying effective managerial and pedagogical practices to stimulate and implement the innovative activity of the subjects of the educational process. Also presented is information obtained using the Ishikawa causal diagram on a set of factors that restrain the participation of teachers in innovative activities, limiting their innovative activity.