Transformation of grape (Vitis vinifera L.) zygotic-derived somatic embryos and regeneration of transgenic plants

Scorza, R.; Cordts, John; Ramming, David W.; Emershad, Richard L.

doi:10.1007/bf00231944

Cited by 55 publications

(42 citation statements)

References 16 publications

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“…These results are presumably due to both the toxic effects of PA and disturbance by PA oxidization, because previous reports showed that phenolic compounds and their oxidation cause the browning of plant tissue and physically disturb regeneration (Madhusudhanan and Rahiman, 2000;Tang and Newton, 2004;Johkan et al, 2008). The transformation of many fruit crops, such as apple, grapevine, kiwifruit, and persimmon, depends on adventitious bud regeneration from leaves or calluses in vitro (Maheswaran et al, 1992;Matsuta et al, 1993;Scorza et al, 1995;Tao et al, 1997;Koshita et al, 2008). However, our results suggested that it is difficult to regenerate transgenic plants with DkMyb4 via adventitious buds in vitro.…”

Section: Application Of Ectopic Dkmyb4 Expressionmentioning

confidence: 68%

DkMyb4 Is a Myb Transcription Factor Involved in Proanthocyanidin Biosynthesis in Persimmon Fruit

et al. 2009

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Proanthocyanidins (PAs) are secondary metabolites that contribute to the protection of the plant and also to the taste of the fruit, mainly through astringency. Persimmon (Diospyros kaki) is unique in being able to accumulate abundant PAs in the fruit flesh. Fruits of the nonastringent (NA)-type mutants lose their ability to produce PA at an early stage of fruit development, while those of the normal astringent (A) type remain rich in PA until fully ripened. The expression of many PA pathway genes was coincidentally terminated in the NA type at an early stage of fruit development. The five genes encoding the Myb transcription factor were isolated from an A-type cultivar (Kuramitsu). One of them, DkMyb4, showed an expression pattern synchronous to that of the PA pathway genes in A-and NA-type fruit flesh. The ectopic expression of DkMyb4 in kiwifruit (Actinidia deliciosa) induced PA biosynthesis but not anthocyanin biosynthesis. The suppression of DkMyb4 in persimmon calluses caused a substantial down-regulation of the PA pathway genes and PA biosynthesis. Furthermore, analysis of the DNA-binding ability of DkMyb4 showed that it directly binds to the MYBCORE cis-motif in the promoters of the some PA pathway genes. All our results indicate that DkMyb4 acts as a regulator of PA biosynthesis in persimmon and, therefore, suggest that the reduction in the DkMyb4 expression causes the NA-type-specific down-regulation of PA biosynthesis and resultant NA trait.

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Section: Application Of Ectopic Dkmyb4 Expressionmentioning

confidence: 68%

DkMyb4 Is a Myb Transcription Factor Involved in Proanthocyanidin Biosynthesis in Persimmon Fruit

et al. 2009

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“…The protocols used for transformation were modified from those described by Scorza et al (1995). Overnight cultures of Agrobacterium strain C58sZ707 or LBA4404 were grown in LB medium at 28 ° C in a shaking incubator.…”

Section: (43k)mentioning

confidence: 99%

“…~'From left to right: NPTII, Neomycin phosphotransferase II; 2enh, double cauliflower mosaic virus 35S enhancers; 35S or double 35S, CaMV 35S promoter; A1MV or CMV, alfalfa mosaic virus or cucumber mosaic virus -C 5' untranslated leader sequences, respectively; 35S or NOS, CaMV 35S or nopaline synthase terminators, respectively; GUS = 13-glucuronidase. al., 1995;Scorza et al, 1995Scorza et al, , 1996, although relatively few scion and rootstock genotypes were transformed and the regeneration rate has generally been low.…”

Section: Introductionmentioning

confidence: 99%

Transformation of five grape rootstocks with plant virus genes and a virE2 gene from Agrobacterium tumefaciens

Xue

Ling

Reid

et al. 1999

In Vitro Cell.Dev.Biol.-Plant

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SUMMARYTo facilitate the development of transgenic grapevines that are resistant to grapevine fanleaf virus (GFLV), grapevine leafroll-associated closterovirus (GLRaV-3) and crown gall diseases, we developed a rapid system for regenerating rootstocks: Couderc 3309, Vitis riparia 'Gloire de Montpellier', Teleki 5C, Millardet et De Grasset 101-14, and 110 Richter via somatic embryogenesis. Embryo culture and grape regeneration were accomplished with four media. Embryogenic calluses from anthers were induced in the initiation medium [MS basic medium containing 20 g sucrose per L, 1.1 mg 2,4-dichlorophenoxyacetic acid (2,4-D) per L, 0.2 mg N6-benzyladenine (BA) per L, and 0.8% Noble agar]. The percentage of anthers that developed into embryogenic calli ranged from 2 to 16.3% depending on the rootstock. Calluses with early globular stage embryos were cocuhivated with Agrobacterium tumefaciens strain C58Z707 containing the gene constructs of interest. The genes were sense-oriented translatable and antisense coat protein genes from GFLV and GLRaV-3, a truncated HSP90-related gene of , and a virE2 del B gene from A. tumefaciens strain C58. Twenty independent transformation experiments were performed on five rootstocks. After 3-4 mo. under kanamycin selection, secondary embryos were recovered on differentiation medium (1/2 MS salts with 10 g sucrose per L, 4.6 g glycerol per L, and 0.8% Noble agar). Embryos that were transformed were regenerated on a medium containing MS salts with 20 g sucrose per L, 4.6 g glycerol per L, 1 g casein hydrolysate per L, and 0.8% Noble agar. Elongated embryos were then transferred to a rooting medium supplemented with 0.1 mg BA per L, 3 g activated charcoal per L, 1.5% sucrose, and 0.65% Bacto agar. A total of 928 independent putative transgenic plants were propagated in the greenhouse. All plants were tested for neomycin phosphotransferase II expression by enzyme-linked immunosorbent assay (ELISA). The presence of transgenes was assessed by polymerase chain reaction and Southern analysis. ELISA revealed various levels of expression of GFLV coat protein in transgenic plants of Couderc 3309. The transgenic rootstocks that have been generated are being screened to determine whether transgenes have conferred resistance to the virus and crown gall diseases.

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“…Um exemplo de transferência de grupos fosfato é o gene neo, que é amplamente usado como marcador de seleção na transformação de plantas (Fraley et al, 1983). O neo é responsável pela produção da enzima neomicina fosfotransferase II (NPTII (Scorza et al, 1995).…”

Section: Avaliação Da Eficiência De Transformaçãounclassified

“…Pode-se considerar que a presença de canamicina favorece a multiplicação de células transformadas na competição com células não transformadas, mesmo se permitir a regeneração de um grande número de falsos transformantes (Scorza et al, 1995). Em amendoim considerou-se que a inibição de crescimento pela canamicina era tão severa nos explantes inoculados com Agrobacterium como nos não inoculados, optando-se então pela não utilização do agente seletivo.…”

Section: Avaliação Da Eficiência De Transformaçãounclassified

Regeneração de plantas de Phaseolus vulgaris L. a partir de calos e transformação genética via Agrobacterium.

Guidolin¹

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Transformation of grape (Vitis vinifera L.) zygotic-derived somatic embryos and regeneration of transgenic plants

Cited by 55 publications

References 16 publications

DkMyb4 Is a Myb Transcription Factor Involved in Proanthocyanidin Biosynthesis in Persimmon Fruit

DkMyb4 Is a Myb Transcription Factor Involved in Proanthocyanidin Biosynthesis in Persimmon Fruit

Transformation of five grape rootstocks with plant virus genes and a virE2 gene from Agrobacterium tumefaciens

Regeneração de plantas de Phaseolus vulgaris L. a partir de calos e transformação genética via Agrobacterium.

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