Transformation of Human Blood Lymphocytes under the Influence of Smallpox Antigen

Gurvich, E B; Svet-Moldavskaya, I. A.

doi:10.1038/2201050a0

Cited by 8 publications

(3 citation statements)

References 5 publications

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“…The lack of correlation between the titer of serum antibodies to tularemia antigen and the lymphocyte response to the Francisella preparation is not unexpected, as stimulation of lymphocytes from immunized individuals is believed to estimate cell-mediated immunity predominantly (19,28). It may be worthwhile eval- 10 Lymphocytes from some of the vaccinated individuals responded only slightly or not at all to the Francisella preparation. Obviously, this may have been due to an individual variability in the immune response to the tularemia vaccine.…”

Section: Discussionmentioning

confidence: 98%

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Stimulation of human lymphocytes by a vaccine strain of Francisella tularensis

Tärnvik¹,

Löfgren²

1975

Infect Immun

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An immune response to Francisella tularensis was demonstrated in man by the lymphocyte stimulation test. Peripheral blood lymphocytes were obtained from 26 individuals vaccinated with a viable tularemia vaccine, from 29 unvaccinated individuals, and from two patients who had recently undergone tularemia. The lymphocytes were incubated in the presence of various dilutions of heat-killed bacteria of the vaccine strain. The bacteria induced a deoxyribonucleic acid synthesis in the lymphocytes from 18 of the vaccinated individuals and from the two patients which was higher than that in the lymphocytes from any of the unvaccinated individuals. The deoxyribonucleic acid synthesis was maximal after about 6 days of incubation irrespective of concentration of bacteria. Lymphocytes from vaccinated and unvaccinated individuals were stimulated by two unrelated agents, tuberculin purified protein derivative and pokeweed mitogen. Lymphocytes from the vaccinated individuals did not show a higher response to these agents than did those of the unvaccinated. This suggests that the lymphocyte response to the Francisella bacteria was not due to a nonspecific activation. The vaccine-induced lymphocyte stimulation did not correlate with serum antibodies agglutinating F. tularensis antigen.

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Section: Discussionmentioning

confidence: 98%

“…However, in vitro reactions of cellmediated immunity such as the lymphocyte stimulation or leukocyte migration tests have not been evaluated. These reactions have been useful in demonstrating an immune response in man to other intracellular parasites (10,17,23,24).…”

mentioning

confidence: 99%

Stimulation of human lymphocytes by a vaccine strain of Francisella tularensis

Tärnvik¹,

Löfgren²

1975

Infect Immun

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“…Recent evidence suggests that the inflammatory reaction occurring in viral infections is an expression of the cell-mediated immune response to viral antigens (2,12). Moreover, the involvement of cell-mediated immunity in recovery from poxvirus infections has been well documented (5,11,18). Therefore, the aim of this study was to determine the effect of the cellmediated immune system on the evolution of vaccinia meningitis after i.c.…”

mentioning

confidence: 99%

Effect of cell-mediated immune factors on the replication of an attenuated temperature-sensitive mutant of vaccinia virus

Keller¹,

Drillien²,

Kirn³

1979

Infect Immun

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Studies of the in vivo multiplication of an attenuated temperature-sensitive strain of vaccinia virus (ts2) indicated that temperature sensitivity alone could not account for the attenuation. Immunodepressive treatment of intracerebrally inoculated mice had a dramatic stimulatory effect on the multiplication of the attenuated strain and suggested that establishment of ts2 infection in the mice was hindered by host defense mechanisms mediated by cellular elements. Experiments carried out in vitro with the 51Cr release assay showed that cells infected with the ts2 mutant represented more susceptible targets to the cytotoxic action of immune spleen cells than cells infected with the parent vaccinia strain. Moreover, it appeared that ts2 replication, compared with wild-type replication in vitro, was more inhibited by the immune spleen cells. Although this work did not evaluate the role of specifically sensitized lymphocytes within the lesions, indirect evidence suggests that lymphocytes could cause a more effective halt in ts2 virus multiplication than in wild-type multiplication.

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Pocken(Pox)- und verwandte Viren

Haagen

1974

Viruskrankheiten Des Menschen

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Transformation of Human Blood Lymphocytes under the Influence of Smallpox Antigen

Cited by 8 publications

References 5 publications

Stimulation of human lymphocytes by a vaccine strain of Francisella tularensis

Stimulation of human lymphocytes by a vaccine strain of Francisella tularensis

Effect of cell-mediated immune factors on the replication of an attenuated temperature-sensitive mutant of vaccinia virus

Pocken(Pox)- und verwandte Viren

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