Transformation of Human Leukocytes by Cocultivation with an Adult T Cell Leukemia Virus Producer Cell Line

Yamamoto, Naoki; Okada, Masuo; Koyanagi, Yoshio; Kannagi, Mari; Hinuma, Yorio

doi:10.1126/science.6980467

Cited by 380 publications

(178 citation statements)

References 8 publications

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“…The HTLV-1-uninfected T-cell leukemia cell lines Jurkat and MOLT-4, the HTLV-1-infected T-cell lines MT-2, 29 MT-4, 30 SLB-1 31 and HUT-102 12 were maintained in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 50 IU/mL penicillin and 50 lg/mL streptomycin (Sigma-Aldrich) at 37°C in 5% CO 2 . MT-2, MT-4 and SLB-1 are HTLV-1-transformed T-cell lines, established by an in vitro coculture protocol.…”

Section: Cell Linesmentioning

confidence: 99%

Retracted: Overexpression of Aurora A by loss of CHFR gene expression increases the growth and survival of HTLV‐1‐infected T cells through enhanced NF‐κB activity

Tomita

Toyota

Ishikawa

et al. 2009

Intl Journal of Cancer

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Retraction: The following article has been retracted through agreement between the first author and several coauthors, the journal Editor‐in‐Chief, Peter Lichter, and John Wiley & Sons, Ltd.: Tomita M, Toyota M, Ishikawa C, Nakazato T, Okudaira T, Matsuda T, Uchihara JN, Taira N, Ohshiro K, Senba M, Tanaka Y, Ohshima K, Saya H, Tokino T, Mori N. (2009). Overexpression of Aurora A by loss of CHFR gene expression increases the growth and survival of HTLV‐1‐infected T cells through enhanced NF‐kappaB activity. Int J Cancer; 124 (June (11)): 2607‐2615, published online on 12 JAN 2009 in Wiley Online Library (www.onlinelibrary.wiley.com). After an investigation the retraction has been agreed due to inappropriate duplication of images and overlap with other published work.

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Section: Cell Linesmentioning

confidence: 99%

Retracted: Overexpression of Aurora A by loss of CHFR gene expression increases the growth and survival of HTLV‐1‐infected T cells through enhanced NF‐κB activity

Tomita

Toyota

Ishikawa

et al. 2009

Intl Journal of Cancer

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“…Efficient spread of HTLV-1 requires cell contact [45,46]. Lymphocytes naturally infected with HTLV-1 produce very few cell-free HTLV-1 virions, and one in 10 5 to 10 6 virions is estimated to be infectious [38,39].…”

Section: Fusion Ability Of Htlv-1-infected Rat Cellsmentioning

confidence: 99%

CRM1, an RNA transporter, is a major species-specific restriction factor of human T cell leukemia virus type 1 (HTLV-1) in rat cells

Zhang

Hakata

Tanaka

et al. 2006

Microbes and Infection

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Rat ortholog of human CRM1 has been found to be responsible for the poor activity of viral Rex protein, which is essential for RNA export of human T cell leukemia virus type 1 (HTLV-1). Here, we examined species-specific barrier of HTLV-1 by establishing rat cell lines, including both adherent and CD4 + T cells, which express human CRM1 at physiological levels. We demonstrated that expression of human CRM1 in rat cells is not harmful to cell growth and is sufficient to restore the synthesis of the viral structural proteins, Gag and Env, at levels similar to those in human cells. Gag precursor proteins were efficiently processed to the mature forms in rat cells and released into the culture medium as sedimentable viralparticles. An HTLV-1 pseudovirus infection system suggested that the released virus particles are fully infectious. Our newly developed reporter cell system revealed that Env proteins produced in rat cells are fully fusogenic, which is the basis for cell-cell HTLV-1 infection.Moreover, we show that the early steps in infection, from post-entry uncoating to integration into the host chromosomes, occur efficiently in rat cells. These results, in conjunction with reports describing efficient entry of HTLV-1 into rat cells, may indicate that HTLV-1 is unique in that its major species-specific barrier is determined by CRM1 at a viral RNA export step.These observations will enable us to construct a transgenic rat model expressing human CRM1 that is sensitive to HTLV-1 infection.

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“…These cells were treated with mitomycin C as described previously. 22 They were cocultured with the IL-2 dependent human T-cell line, TY8-3, 27 established from a thymoma specimen from a patient with myasthenia gravis, in RPMI-1640 medium containing 10% FBS and 10 ng/ml IL-2. After growing cells were expanded, the cells were maintained in the absence of IL-2.…”

Section: Cocultivation Of Interleukin 2 (Il-2) Dependent Ty8-3 and Htmentioning

confidence: 99%

Constitutive expression of the cyclooxygenase-2 gene in T-cell lines infected with human T cell leukemia virus type I

Mori

Inoue

Yoshida³

et al. 2001

Int. J. Cancer

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Cyclooxygenase-2 (COX-2), an inducible enzyme that catalyzes the formation of prostaglandins and other eicosanoids from arachidonic acid, is constitutively expressed in several human carcinomas. COX-2 expression, however, has not been extensively studied in leukemia. Human T cell leukemia virus type I (HTLV-I) is the etiologic agent of adult T cell leukemia, an aggressive form of human T cell malignancy. We studied COX-2 mRNA expression in various human Tcell lines. Northern blot analysis revealed that COX-2 mRNA steady-state levels were high in 4 of 7 T-cell lines infected with HTLV-I. COX-2 mRNA, however, was not expressed in any of 3 HTLV-I-negative T-cell lines. We also confirmed COX-2 expression in 6 of 7 HTLV-I-positive T-cell lines by reverse transcription-PCR. HTLV-I Tax is known to increase the expression of cellular genes and thus we assayed Tax for its ability to increase transcription from the COX-2 promoter. Although Tax increased transcription of the COX-2 promoter in a T-cell line, Tax expression did not induce COX-2 mRNA expression, indicating that Tax alone is not sufficient for significant accumulation of COX-2 mRNA, which probably requires an additional viral protein. To evaluate the potential role of COX-2 in T cells, the HTLV-Iinfected T-cell lines were treated with NS398, a selective COX-2 inhibitor. NS398 treatment inhibited proliferation and induced apoptosis of HTLV-I-infected T-cell lines and downregulated Bcl-2 and Bcl-x L mRNA expression, followed by chromosomal DNA fragmentation. Our data suggest that COX-2 is expressed selectively in T-cell lines infected with HTLV-I and that this gene may play a role in cell survival.

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Transformation of Human Leukocytes by Cocultivation with an Adult T Cell Leukemia Virus Producer Cell Line

Cited by 380 publications

References 8 publications

Retracted: Overexpression of Aurora A by loss of CHFR gene expression increases the growth and survival of HTLV‐1‐infected T cells through enhanced NF‐κB activity

Retracted: Overexpression of Aurora A by loss of CHFR gene expression increases the growth and survival of HTLV‐1‐infected T cells through enhanced NF‐κB activity

CRM1, an RNA transporter, is a major species-specific restriction factor of human T cell leukemia virus type 1 (HTLV-1) in rat cells

Constitutive expression of the cyclooxygenase-2 gene in T-cell lines infected with human T cell leukemia virus type I

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